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Preparation and quality detection method of compound prescription cortex phellodendri chinensis fluid

A quality inspection method, the technology of compound Phellodendron liquid, applied in the field of preparation and quality inspection of compound Phellodendron liquid, can solve the problems that are not conducive to the health of laboratory personnel, and there is no determination of forsythia content, and achieve high accuracy and linearity of recovery test. Good relationship, good reproducibility and stability of the effect

Inactive Publication Date: 2015-03-11
SHANDONG HANFANG PHARMA
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Thin-layer chromatography was used to determine the content of berberine hydrochloride and the qualitative identification of forsythia and Phellodendron cortex. The developing agent contained benzene and other substances that are highly toxic to the human body, which is not conducive to the health of laboratory personnel. At the same time, forsythia was not tested. As there is honeysuckle in the prescription, and there are differences between honeysuckle and mountain silver flower in terms of medicinal history, plant morphology, medicinal properties, chemical composition, etc., especially mountain silver flower contains a large amount of saponins, there may be hemolysis and other safety risks, laying hidden dangers for clinical use

Method used

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  • Preparation and quality detection method of compound prescription cortex phellodendri chinensis fluid
  • Preparation and quality detection method of compound prescription cortex phellodendri chinensis fluid
  • Preparation and quality detection method of compound prescription cortex phellodendri chinensis fluid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] A kind of compound cortex cortex liquid, its raw material consists of:

[0061] Forsythia 80 parts by weight Cortex Phellodendri 40 parts by weight

[0062] Honeysuckle 40 parts by weight Dandelion 40 parts by weight

[0063] Centipede 2.4 parts by weight.

[0064] A preparation method of compound Phellodendron Phellodendron comprises the following steps:

[0065] (1) Weigh the five traditional Chinese medicines in the prescription according to the weight components, add water to decoct and extract three times, the first time is 1 hour, the second time is 45 minutes, and the third time is 30 minutes;

[0066] (2) Combine the decoctions, filter, and concentrate the filtrate to a clear paste with a relative density of 1.10-1.15 (measured at a temperature of 50°C);

[0067] (3) Add ethanol to make the ethanol content reach 70%, let it stand for 24 hours, filter, and concentrate the filtrate under reduced pressure until there is no ethanol, and obtain the clear liquid fo...

experiment example 1

[0105] Experimental example 1: Research experiment on identification methods of various raw materials

[0106] Prepare compound Phellodendron phellodendron preparation according to the standard of compound phellodendron phellodendron preparation, test the identification method of various crude drugs wherein, experimental basis and result are as follows:

[0107] 1. Refer to the existing compound Phellodendron Phellodendron preparation standard to carry out identification test on forsythia. The results show that in the three batches of samples, spots of the same color appear on the positions corresponding to the chromatogram of the reference medicinal material, but the chromatographic spots of the reference medicinal material are not clear, and the chromatographic spots of the control medicinal material are not clear, and forsythia The glycoside reference substance was not developed. Therefore, it is not used as a part of the quality detection method of the compound Phellodendr...

experiment example 2

[0111] Experimental example 2: HPLC method to check whether the raw material of the compound Phellodendron phellodendron is made of mountain silver flower

[0112] 1. Instruments, reagents and test samples

[0113] Instruments, high performance liquid chromatography: Agilent 1200 high performance liquid chromatography (ELSD detector); Sartorius CP225D electronic balance.

[0114] Control medicinal materials: honeysuckle, mountain silver flower.

[0115] Reference substance: Lonicera saponin B reference substance, Dipsacus saponin B reference substance.

[0116] Sample: Compound Cortex Phellodendron Liquid.

[0117] 2. Selection of mobile phase

[0118] Referring to the inspection method of Yinhua Zhongshan in the 2015 edition of "Research on Specific Detection Methods of Multi-source Chinese Medicinal Materials in Chinese Proprietary Medicine" project "Research on Specific Detection Methods of Multi-source Chinese Medicinal Materials in Chinese Proprietary Medicine", it is ...

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Abstract

The invention discloses preparation and quality detection method of compound prescription cortex phellodendri chinensis fluid. The quality detection method comprises the identification of forsythia fruit, cortex phellodendri, honeysuckle and dandehon herb, the checking of lonicerae flos, and the content determination of forsythin and berberine hydrochloride. According to the determination method, spots with the same colors are formed in a qualified test article thin-layer chromatogram and in corresponding positions of a reference substance thin-layer chromatogram, and negative control is interference-free, and the method is good in selectivity, simple and accurate. The checking method of lonicerae flos adopts a high performance liquid chromatography, is good in specificity, and can distinguish the situation that lonicerae flos is adopted in the compound prescription cortex phellodendri chinensis fluid to pretend honeysuckle flower to charge material. The content determination of forsythin and berberine hydrochloride is good in linear relation and high in recovery test accurancy, has good repeatability and stability, can accurately and strictly detect the quality of the compound prescription cortex phellodendri chinensis fluid, and guarantees a high quality standard level of the compound prescription cortex phellodendri chinensis fluid.

Description

Technical field [0001] The present invention involves the preparation and quality testing method of a pharmaceutical composition, and specially involves the preparation and quality testing method of a compound yellow cypress liquid. Background technique [0002] Compound Huangbai fluid, Chinese medicine quasi -word Z10950097, the main components are: forsythia, cork, honeysuckle, dandelion.It has the functions of clearing heat and detoxifying, swelling and pain, removing corruption, and anti -Gram -positive bacteria, Gram -negative bacteria, anti -inflammatory and promoting wound healing.effect.It is suitable for common diseases such as osteomyelitis, bone tuberculosis trail, vasculitis and other common diseases such as trauma infections after ulcer ulcers. It is a special effect of clinical purulent infection and promoting healing. [0003] The quality testing standards of the current compound corkyl fluid include the qualitative identification of forsythia, cork and cork, and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/756A61P17/02A61P31/04A61P31/06A61P19/08A61P9/14G01N30/90G01N31/02A61K35/648
CPCA61K36/634A61K35/648A61K36/288A61K36/355A61K36/756A61K2236/331A61K2236/39G01N30/90G01N2030/027
Inventor 秦文基
Owner SHANDONG HANFANG PHARMA
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