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Use of trichothecenes in resisting tobacco pathogenic fungi

A technology of tobacco pathogenic fungi and trichothecenes, which is applied in the fields of application, fungicides, and chemicals for biological control, etc., can solve problems such as singleness, decline in resistance of tobacco varieties, and aggravation of tobacco hazards, so as to improve the quality of tobacco yield effect

Inactive Publication Date: 2016-03-23
INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In tobacco production, due to the single planting variety structure, the decline of tobacco variety resistance, and the emergence of the "three R" problem mainly caused by chemical pesticide control, the drug resistance of diseases and insect pests has increased, resulting in a gradual increase in the harm of diseases and insect pests to tobacco. Sustainable Development of Tobacco Agriculture

Method used

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  • Use of trichothecenes in resisting tobacco pathogenic fungi
  • Use of trichothecenes in resisting tobacco pathogenic fungi
  • Use of trichothecenes in resisting tobacco pathogenic fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] This example is used to illustrate the effect of using trichothecenes to inhibit the growth of tobacco pathogenic fungi.

[0027] The spores of Alternaria alternata and Alternaria alternata were prepared to form a spore suspension of appropriate concentration (under a 10×10 low power microscope, 30-40 spores per field of view). Trichothecene was prepared into a certain concentration of acetone aqueous solution, then mixed with the spore suspension in equal volume, and one drop was added dropwise on the concave glass slide. Each treatment was repeated 3 times. After moisturizing culture at 28°C for 18 hours, check the control spores Germination situation. When the germination rate of the control spore reaches 85%, check the spore germination rate of all treatments. The spore germ tube length is greater than half of the spore length as germination, and the trichothecene concentration when the spore germination inhibition rate is 100% is found. That is the minimum inhibitory...

Embodiment 2

[0029] This example is used to illustrate the effect of using trichothecenes to inhibit the growth of tobacco pathogenic fungi hyphae.

[0030] The plate growth rate method was used to determine the inhibitory effect of trichothecenes on the growth of the hyphae of Alternaria alternata and Alternaria alternata. The steps are as follows:

[0031] Prepare trichothecenes with sterile water at concentrations of 500μg / ml, 250μg / ml, 125μg / ml, 62.5μg / ml, 31.25μg / ml, 15.625μg / ml, 7.8125μg / ml, 3.90625μg / ml Solution, use sterile water as control (CK).

[0032] Pour 10 mL of each diluted solution into 8 bottles of sterilized culture medium (each bottle contains 90 mL of culture medium, and the drug solution is diluted 10 times). After mixing, pour the culture medium into a petri dish with a diameter of 9 cm. A series of concentration gradient drug-containing culture plates, 5 replicates for each concentration, and stand by after condensation. Use a sterile stainless steel puncher with a diame...

Embodiment 3

[0042] This example is used to illustrate the determination of the effect of trichothecenes on the prevention and control of tobacco pathogenic fungi infection in greenhouse pots.

[0043] Select healthy tobacco plants with the same growth at the 8-9 leaf stage to determine the protective and therapeutic effects.

[0044] Protective effect: spray trichothecene (sprayed at a concentration of 200μg / mL), 24 hours later, the spray method inoculation concentration is 3×10 5 A / mL spore suspension of tobacco pathogenic fungi (Alternaria alternata or Alternaria alternata), a 1% glucose solution was set as a blank control, and the spraying rates of the three were all 50 mL / plant. After inoculation, they were placed in a 28°C incubator and kept for 6 days.

[0045] Therapeutic effect: inoculation concentration of spray method is 3×10 5 A / mL spore suspension of tobacco pathogenic fungi (Alternaria alternata or Alternaria alternata), set a 1% glucose solution as a blank control, and then placed ...

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Abstract

The invention discloses a method for inhibiting tobacco pathogenic fungus growth. The method comprises contact between trichothecin and tobacco pathogenic fungi. The invention discloses a method for preventing and treating tobacco fungal diseases. The method comprises applying trichothecin to tobacco plants. The invention also discloses a use of trichothecin in resistance to tobacco fungal diseases. The trichothecin can effectively prevent and treat tobacco fungal diseases (especially such as tobacco brown spot and / or tobacco black shank) and has a great meaning for preventing and treating tobacco diseases and improving a tobacco yield.

Description

Technical field [0001] The present invention relates to the use of trichothecenes in anti-tobacco pathogenic fungi. Specifically, it relates to a method for using trichothecenes to inhibit the growth of tobacco pathogenic fungi, prevent and treat tobacco fungal diseases, and the use of trichothecenes in resistance Use in fungal diseases of tobacco. Background technique [0002] Tobacco pathogenic fungi are one of the main culprits hindering tobacco production. Among them, tobacco brown spot disease is one of the main diseases in tobacco production in my country. The disease has occurred in all tobacco-producing areas in China, with the most serious occurrence in Shandong, Henan, Anhui, Heilongjiang, Jilin and other places; followed by Sichuan, Yunnan, Guizhou, Liaoning, Shaanxi and other tobacco areas. In addition, the oriental tobacco producing areas in Guangdong and the sun-cured tobacco producing areas in Zhejiang are also more harmful. [0003] Tobacco black shank is one of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/90A01P3/00
Inventor 杨佩文肖志新曾莉胡志明尚慧刘芮李家瑞郭应成金桂梅姜吉飞孙加利
Owner INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
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