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Trichoderma longibrachiatum and application thereof in preventing and treating vegetable diseases

A technology for Trichoderma longifolia and vegetable diseases, applied in the direction of microorganisms, microorganisms, fungi, etc., can solve the problems of destroying agricultural ecosystems, environmental pollution, etc., and achieve the effects of environmental friendliness, environmental improvement, and vegetable crop safety

Inactive Publication Date: 2012-12-19
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, chemical agents are mostly used for prevention and control in production, but long-term and large-scale use of pesticides causes pathogenic bacteria to develop resistance, and seriously damages agricultural ecosystems, causing environmental pollution

Method used

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  • Trichoderma longibrachiatum and application thereof in preventing and treating vegetable diseases
  • Trichoderma longibrachiatum and application thereof in preventing and treating vegetable diseases
  • Trichoderma longibrachiatum and application thereof in preventing and treating vegetable diseases

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Effect test

Embodiment 1

[0018] Embodiment 1, acquisition and preservation of Trichoderma longibrachiatum (Trichoderma longibrachiatum) Gu 4-5

[0019] 1. Sampling

[0020] Soil samples were collected from Beijing, Hebei, Xinjiang, Shandong, Hubei, etc.

[0021] 2. Isolation of strains

[0022]Strains were isolated by the coating method. Weigh 10g of each soil sample, pour it into a sterile 500ml Erlenmeyer flask with a little Tween 80, glass beads and 100ml of distilled water, shake well, label, and shake for 30 minutes with a shaker. Then use a micro-syringe to suck 5ml of the soil suspension into a 250ml Erlenmeyer flask containing 45ml of sterilized distilled water, shake well, then suck 1ml from the 250ml Erlenmeyer flask and add it to a test tube containing 9ml of sterilized distilled water for doubling dilution , the sample was serially diluted to 10 -4 and 10 -5 Take 0.1ml of each sample and drop it on a PDA plate, spread it, and incubate it at 28°C for 48-72 hours, pick out the colony th...

Embodiment 2

[0046] Embodiment 2, the application of Trichoderma longifolia Gu 4-5 in the prevention and treatment of vegetable diseases

[0047] By artificial inoculation, the effects of Trichoderma longifolia Gu 4-5 on scab pathogens, fusarium wilt pathogens, brown spot pathogens, blight pathogens, gray mold pathogens, sheath blight Inhibition of pathogenic bacteria and sclerotinia pathogenic bacteria.

[0048] 3% Jinggangmycin aqueous solution: Tonglu Huifeng Biochemical Co., Ltd., Zhejiang Province. 50% dimethomorph: Hebei Shangrui Chemical Co., Ltd. 75% chlorothalonil: Xinyi Fengle Chemical Co., Ltd. 40% Fuxing EC500: DuPont China Group Co., Ltd. 40% pyrimethanil suspension concentrate: Yantai Keda Chemical Co., Ltd. 70% thiophanate-methyl: Shandong Huayang Pesticide Chemical Group Co., Ltd. Jinggangmycin solution: 0.01 g of 3% Jinggangmycin aqueous solution was dissolved in 20 ml of water to obtain Jinggangmycin solution. Dimethomorph solution: 0.01 g of 50% dimethomorph was di...

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Abstract

The invention discloses trichoderma longibrachiatum and application thereof to preventing and treating vegetable diseases. The collection number of the trichoderma longibrachiatum is CGMCC No.4708. The trichoderma longibrachiatum can be applied to preventing and treating vegetable diseases. The trichoderma longibrachiatum Gu 4-5 (or a preparation using the trichoderma longibrachiatum Gu 4-5 as anactive ingredient) is environment-friendly, can inhibit medicament resistance development of disease pathogeny, contributes to popularization of green food and organic agriculture, has low cost and no pollution, and is safe for vegetable crops. Antagonistic microorganisms are introduced into soil, so fungal diseases can be effectively prevented and treated through biological prevention and control, the environment can be effectively improved, an agroecological system can be maintained to be balanced, and strong support is provided for sustainable development.

Description

technical field [0001] The invention relates to a long handle trichoderma and its application in preventing and treating vegetable diseases. Background technique [0002] At present, my country's vegetable planting area has exceeded 150 million mu, of which greenhouse vegetables and protected horticultural plants have exceeded 20.1 million mu. Due to the expansion of vegetable greenhouse cultivation area and continuous cropping, favorable ecological conditions are provided for the occurrence of diseases and insect pests. Vegetable wilt, gray mold, blight and other fungal diseases commonly occur in vegetable planting. When serious, they will cause a substantial reduction in yield, which is a thorny problem that farmers often encounter in the process of seedling cultivation and planting. [0003] Fusarium wilt is a kind of whole-plant disease that infects from the root and parasitizes in the vascular bundle at the seedling stage, but it is generally less symptomatic at the se...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/885
Inventor 顾金刚李世贵阮志勇张晓霞马晓彤张瑞颍左学梅王爱民
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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