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A kind of Pseudomonas aeruginosa and application thereof

A technology of Pseudomonas aeruginosa and bacterial strains, applied in Pseudomonas aeruginosa and its application fields, can solve the problems of difficult growth and reproduction, lack of adaptability, poor tolerance of formation water salinity in reservoirs, etc., and achieve improved flooding Oil efficiency, social production friendliness, effect of increasing effective permeability

Active Publication Date: 2017-09-01
PETROCHINA CO LTD
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, the Pseudomonas aeruginosa screened from the common environment is not adaptable to the reservoir environment, especially the tolerance to the salinity of reservoir formation water is very poor, and it is difficult to grow and reproduce when the salinity exceeds 10g / l. Unable to carry out microbial oil recovery engineering in high salinity reservoirs

Method used

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  • A kind of Pseudomonas aeruginosa and application thereof
  • A kind of Pseudomonas aeruginosa and application thereof
  • A kind of Pseudomonas aeruginosa and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Acquisition of Pseudomonas aeruginosa QHH S1-27-2

[0018] Collect oil and water samples from production wells in Qinghai Oilfield, fill them in sterile polystyrene bottles, and bring them back to the laboratory.

[0019] (1) Enrichment: Take 10g of oil and water sample and add it to the enrichment medium, put 100mL of fermentation medium into a 250mL conical flask, seal the bottle mouth, 50℃, 120r / min, constant temperature shaking culture for 3d.

[0020] (2) Blood Plate Separation

[0021] After enrichment culture, observe the fermentation broth morphology, draw 0.1 mL from the shake flask culture that can disperse oil into oil droplets for 10 -5 -10 -7 Gradient dilution, and then use a sterilized spreader to spread the blood plate evenly, and cultivate for 2 days in a 37°C constant temperature incubator. Using biosurfactants to lyse erythrocytes, it appears as a hemolytic circle on the blood plate. Therefore, a single colony that produces a large-diameter hemolyt...

Embodiment 2

[0031] Determination of Crude Oil Deoiling by Pseudomonas aeruginosa QHH S1-27-2

[0032] The crude oil and quartz sand in the Huatugou working area of ​​Qinghai Oilfield were mixed at a volume ratio of 1:1 and aged at 37°C for 1 week. Take 20 g of dried quartz sand with oil, add 50 ml of 3% strain fermentation broth, add Huatugou formation water to the blank control, and cultivate at a constant temperature of 35 °C for 48 hours at a simulated formation temperature. After 0.5, 1, 3, 6, 12, 24, and 48 hours, the crude oil floating on the liquid surface was taken out and measured. The deoiling efficiency of the fermentation broth of the oil-displacing strain was detected. With the prolongation of the action time of the strain fermentation liquid, the oil layer in the beaker added with the oil-displacing microbial fermentation liquid gradually thickened, and the bottom quartz sand gradually revealed its true color, and the deoiling effect was very significant. The deoiling effi...

Embodiment 3

[0036] Determination of the adaptability of Pseudomonas aeruginosa QHH S1-27-2 to the environment

[0037] (1) Determination of strain reproduction ability

[0038]After being inoculated into the medium at 3%, the cells were shaken and cultured at 60 r / min at 35 °C, and the OD value was measured every 4 h. According to the linear relationship between bacterial concentration and OD value, the bacterial concentration corresponding to different OD values ​​was calculated. Pseudomonas aeruginosa QHH S1-27-2 entered the logarithmic growth phase after 8-12h, and entered the stable phase after about 24-32h, and the number of bacteria corresponding to the OD value was greater than 10 7 CFU / mL.

[0039] (2) Determination of the adaptability of strains to temperature

[0040] Three temperature gradients of 30, 35, and 40 °C were designed, inoculated into the medium according to 3% of the inoculum, and the OD value was measured after shaking for 28 hours (the number of bacteria in the...

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Abstract

The present invention relates to a kind of Pseudomonas aeruginosa and its application; The Pseudomonas aeruginosa QHH S1-27-2, its microbial preservation number is CCTCC NO: M2012468; it is applied to high salinity oil reservoir microbial oil recovery Middle; Pseudomonas aeruginosa of the present invention can grow with crude oil as the only carbon source, and the biosurfactant component in its metabolites can reduce the oil-water interfacial tension, increase the effective permeability of the reservoir to a certain extent, and improve the injection water Oil displacement efficiency, enhanced oil recovery.

Description

technical field [0001] The invention relates to Pseudomonas aeruginosa in the field of biotechnology and applications thereof, in particular to a strain of Pseudomonas aeruginosa that produces biosurfactants and its application in microbial oil recovery from high-salinity oil reservoirs. Background technique [0002] Oil is a non-renewable resource, but 60-70% of the remaining oil and residual oil remain in the formation after secondary oil recovery, which is a huge potential for enhancing oil recovery and increasing recoverable reserves. It is estimated that a 1% increase in oil recovery can meet the world's oil consumption for 2 to 3 years. Therefore, how to improve the technical level of tertiary oil recovery has important economic value and strategic significance. [0003] Tertiary oil recovery mainly includes physical oil recovery, chemical oil recovery and microbial oil recovery. Physical oil recovery technology mainly uses high-temperature and high-pressure steam int...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K8/582E21B43/22C12R1/385
CPCC09K8/582C12N1/205C12R2001/385E21B43/16
Inventor 扈福堂张立会周平薛姝雯
Owner PETROCHINA CO LTD
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