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Aquatic plant compound, extraction method of polysaccharide in aquatic plant compound, and novel use of polysaccharide in aquatic plant compound

A technology of aquatic plants and compounds, which is applied in the fields of aquatic plant compounds, preparation of anti-tumor drugs and compounds, can solve the problems of little research on synergistic effects, achieve immune enhancement and anti-tumor effects, high curative effect, and improve extraction efficiency effect

Inactive Publication Date: 2015-02-25
SUZHOU BOLI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Most of the previous studies focused on the single component of a certain polysaccharide, while the compound polysaccharide composed of two or more polysaccharides was used in animals to observe the synergistic effect of its immune function and anti-tumor effect.

Method used

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  • Aquatic plant compound, extraction method of polysaccharide in aquatic plant compound, and novel use of polysaccharide in aquatic plant compound
  • Aquatic plant compound, extraction method of polysaccharide in aquatic plant compound, and novel use of polysaccharide in aquatic plant compound
  • Aquatic plant compound, extraction method of polysaccharide in aquatic plant compound, and novel use of polysaccharide in aquatic plant compound

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Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1: a kind of extraction method of aquatic plant complex polysaccharide comprises the following steps:

[0033] (1) Take 100g each of lotus root, water celery, Gorgon fruit, shiitake mushroom, water chestnut, water shield and water chestnut, and use a medicinal mechanical grinder for high-speed crushing. After crushing the raw materials to 20 mesh, add the crushed powder to ultrapure water 4500mL was subjected to high-temperature ultrasonic treatment for 30 minutes, and the filtrate was collected.

[0034] (2) After adding 4500 mL of ultrapure water to the filter residue and continuing to decoct for 1 hour, it was combined with the filtrate collected in step (1).

[0035] (3) Evaporate the combined solution at 80°C to the remaining 10% of the solution, transfer to a beaker, and cool to room temperature.

[0036] (4) Add 95% ethanol and stir while adding until the final ethanol content in the solution is about 75%.

[0037] (5) After the solution in step (4) ...

Embodiment 2

[0038]Embodiment 2: a kind of extraction method of aquatic plant complex polysaccharide, comprises the following steps:

[0039] (1) Take 100g each of lotus root, water celery, Gorgon fruit, shiitake mushroom, water chestnut, water shield and water chestnut, and use a medicinal mechanical grinder to grind at high speed. After grinding the raw materials to 25 mesh, add the crushed powder to ultrapure water 4900 mL was subjected to high-temperature ultrasonic treatment for 35 minutes, and the filtrate was collected.

[0040] (2) Add 4900 mL of ultrapure water to the filter residue and continue decocting for 1.5 hours, then combine with the filtrate collected in step (1).

[0041] (3) Evaporate the combined solution at 85°C to the remaining 14% of the solution, transfer to a beaker, and cool to room temperature.

[0042] (4) Add 95% ethanol and stir while adding until the final ethanol content in the solution is about 78.5%.

[0043] (5) After the solution in step (4) was left ...

Embodiment 3

[0044] Embodiment 3: a kind of extraction method of aquatic plant complex polysaccharide comprises the following steps:

[0045] (1) Take 100g each of lotus root, water celery, Gorgon fruit, shiitake mushroom, water chestnut, water shield and water chestnut, and use a medicinal mechanical grinder for high-speed crushing. After crushing the raw materials to 30 mesh, add the crushed powder to ultrapure water 5300 mL was subjected to high-temperature ultrasonic treatment for 40 minutes, and the filtrate was collected.

[0046] (2) After adding 5300 mL of ultrapure water to the filter residue and continuing to decoct for 2 hours, it was combined with the filtrate collected in step (1).

[0047] (3) Evaporate the combined solution at 90°C to the remaining 18% of the solution, transfer to a beaker, and cool to room temperature.

[0048] (4) Add 95% ethanol and stir while adding until the final ethanol content in the solution is about 75% to 82%.

[0049] (5) After the solution in ...

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Abstract

The invention discloses an aquatic plant compound, which comprises eight aquatic plants including zizania aquatica, lotus root, cress, gorgon fruit, arrowhead, chufa, brasenia schreberi and water chestnut. The weight ratio of zizania aquatica, lotus root, cress, gorgon fruit, arrowhead, chufa, brasenia schreberi to water chestnut is 2:2:1:2:2:2:1:2. The extraction method disclosed by the invention comprises the following steps: (1), crushing various raw materials at a high speed, and adding ultrapure water to collect a filtrate; (2), adding ultrapure water into filter residues to decoct continuously, and mixing filtrate with the filtrate collected in the step (1); (3), evaporating the mixed liquid till the weight of remained liquid is 10-18% of that of the mixed filtrate, and cooling to room temperature; (4), adding ethanol while stirring; and (5), standing and precipitating the liquid obtained in the step (4), carrying out suction filtrate to remove filtrate, collecting filter residues, washing the filter residues by using ethanol and acetone respectively once, and drying remained filter residues. Polysaccharide in the aquatic plant compound has good anti-tumor activity and low toxic and side effects, and can be used for preparing anti-tumor drugs.

Description

technical field [0001] The present invention relates to a compound, its polysaccharide extraction method and its new application of polysaccharide, especially relates to an aquatic plant compound, its polysaccharide extraction method and its new application of polysaccharide for preparing antitumor drugs, belonging to the technical field of natural medicine . Background technique [0002] "Water Eight Immortals" refers to eight kinds of vegetables, including water bamboo, lotus root, cress, gorgon, arrowhead, water chestnut, water shield, and water chestnut. They are traditionally cultivated aquatic vegetable varieties in our city, mainly distributed in Wuzhong District, Wujiang, and Changshu in our city , Taicang, Zhangjiagang, Kunshan and other places. Due to the long history of planting, there are many varieties, high quality and fine cultivation. It once formed distinctive local characteristics and enjoyed a high reputation at home and abroad, so it was nicknamed the "...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/899A61K31/715A61P35/00
CPCA61K36/899A61K31/715A61K36/185A61K36/23A61K36/62A61K36/884A61K36/89A61K2236/15A61K2236/331A61K2236/51A61K2236/55C08B37/00A61K2300/00
Inventor 吴梦旦许颖朱玮
Owner SUZHOU BOLI BIOTECH
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