Preparation method of magnetic response composite lipase and application in biodiesel synthesis

Abstract

The invention relates to a preparation method of magnetic response composite lipase, and preparation of biodiesel by using the magnetic response composite lipase, and belongs to the technical field of preparation of biodiesel by using immobilized enzyme. According to the preparation method, magnetic nano Fe3O4 is adopted to modify lipase A with 1,3-position specificity, an immobilization technique is adopted to prepare the lipase A with the 1,3-position specificity and lipase B without position specificity into a composite enzyme double-enzyme system, as a method of composite lipase concerted catalysis is adopted, the substrate specificity of single lipase is overcome, the transesterification efficiency in preparing biodiesel by using an enzyme method is improved, recycling and repeated use of enzyme are achieved, and the enzyme activity loss in the reaction process is reduced.

Description

technical field [0001] The invention relates to the technical field of biodiesel preparation by enzymatic method, in particular to the preparation of an immobilized composite double enzyme system with magnetic responsiveness by using two lipases with different catalytic properties and its application in biodiesel synthesis. Background technique [0002] Compared with traditional petrochemical diesel, biodiesel has attracted extensive attention because of its renewable, biodegradable, and environmentally friendly properties. Biodiesel is mainly a long-chain fatty acid ester (fatty acid methyl or ethyl ester) prepared from animal and vegetable oils through transesterification. At present, the preparation methods of biodiesel mainly include chemical method and biological enzymatic method. Chemical methods, mainly alkali-catalyzed methods, have relatively mature production processes and are commonly used methods for preparing biodiesel; however, chemical methods have disadvanta...

AI Technical Summary

Problems solved by technology

[0005]However, the compound enzyme technology currently used in biodiesel production is based on the existing immobilized enzyme, and the price is relatively high. For example, the most widely used one is Novi Shin's Novozym 435, priced at $2,270 per kilogram

On the other hand, since the lipid substrate is hydrophobic, lipase must be combined with the substrate to catalyze the reaction, so the catalytic reaction of lipase on esters can only occur at the oil-water interface.

Therefore, during the reaction process, constant stirring is necessary to ensure sufficient contact between the enzyme and the substrate, but mechanical stirring will have a certain impact on the carrier or the enzyme, resulting in the loss of the enzyme or the reduction of the enzyme activity.

Third, existing natural lipases still have problems such as poor stability during use, and low-carbon alcohols such as methanol are likely to cause enzyme inactivation.

If the nature of natural lipase itself is not changed but only immobilized, it still cannot solve the needs of its industrial production

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