HPLC content determination method for adenosine in semen plantaginis

A technology of adenine nucleoside and determination method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., and can solve problems such as unclear chemical composition

Inactive Publication Date: 2015-01-21
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Although scholars at home and abroad have extensively studied the chemical components and pharmacological effects of Plantago chin...

Method used

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  • HPLC content determination method for adenosine in semen plantaginis
  • HPLC content determination method for adenosine in semen plantaginis
  • HPLC content determination method for adenosine in semen plantaginis

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Experimental program
Comparison scheme
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Embodiment 1

[0144] HPLC method measures the content of adenosine in Semen Plantaginis, and the steps are as follows:

[0145] 1. Experimental equipment and chromatographic conditions

[0146] Chromatograph: Agilent1200 high performance liquid chromatograph

[0147] Detector: UV detector

[0148] Chromatographic column: Prevail C18 (250mm×4.6mm, 5mm)

[0149] Chromatographic conditions: mobile phase: acetonitrile-water volume ratio is 5:95

[0150] Flow rate: 0.1mL / min

[0151] Column temperature: 25°C

[0152] Detection wavelength: 255nm

[0153] Injection volume: 20 μL.

[0154] 2. Methodological investigation

[0155] 2.1. Inspection of linear relationship

[0156]Accurately weigh 0.00604g of the CQZ-1 reference substance, dissolve it in chromatographic acetonitrile-water (5:95) and dilute it to a 50mL volumetric flask, shake well to obtain a standard stock solution. Accurately pipette 12.50, 6.00, 2.50, 1.25, 0.75mL into a 25mL volumetric flask, dissolve with chromatographic a...

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Abstract

Adenosine, cannabiside B and ethyl glucoside are firstly separated and obtained from semen plantaginis, and on the above basis, the invention provides a method of determining the content of adenosine in semen plantaginis by utilizing a HPLC process. The chromatographic conditions of the method comprise that the mobile phase is acetonitrile and water according to the volume ratio of 5:95, the low velocity is 0.1 mL/min, the column temperature is 25 DEG C, the detection wavelength is 255 nm, and the sample size is 20 mu L. The method provides content determination index for completing quality standard of semen plantaginis, and also provides new scientific basis for exploitation and utilization of semen plantaginis.

Description

technical field [0001] The invention relates to the determination of the content of adenosine in psyllium seed by using an HPLC method, and belongs to the field of biomedicine. Background technique [0002] Semen Plantaginis (Semen Plantaginis) is the dry mature seed of Plantago asiatic L. or Plantago depressa Willd. Seed Plantago is first recorded in "Shen Nong's Materia Medica" as a medicinal product. Its flavor glycosides, cold in nature, non-toxic, enter the liver, kidney, lung, and small intestine meridians, and have the functions of clearing away heat, diuresis and treating stranguria, oozing dampness and stopping diarrhea, improving eyesight, and dispelling diarrhea. The effect of phlegm. It is used for hot stranguria and astringent pain, edema and fullness, diarrhea due to summer-heat dampness, conjunctival congestion and distending pain, cough due to phlegm-heat. Psyllium seed has the same origin of medicine and food, and is listed as a list of items that can be u...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 李绪文金永日盖艳喆
Owner JILIN UNIV
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