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A slow-release hydrogen sulfide donor and its preparation method and application

A hydrogen sulfide donor and hydrogen sulfide technology, applied in the field of molecular biology, can solve the problems of fast release rate, unadjustable, and limited application of hydrogen sulfide donors.

Inactive Publication Date: 2017-03-15
ZHONGSHAN HOSPITAL FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Diallyl trisulfide (DATS), an allicin extract, can effectively release hydrogen sulfide in the presence of glutathione (reduced glutathione, GSH), but its release rate is fast, its concentration is high, and it cannot be regulated
Moreover, DATS is insoluble in water, which limits its application as a hydrogen sulfide donor.

Method used

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  • A slow-release hydrogen sulfide donor and its preparation method and application
  • A slow-release hydrogen sulfide donor and its preparation method and application
  • A slow-release hydrogen sulfide donor and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Preparation of Ordered Mesoporous Silica Nanoparticle Material (MSN)

[0028] Add 300 mL of deionized water, 2.1 mL of 2 mol / L sodium hydroxide solution, and 0.6 g of template agent cetyltrimethylammonium bromide (CTAB) into a 500 mL three-necked flask, in a constant temperature water bath at 80°C, 200 rpm / minute mechanical stirring. After 1 h, 3 mL of tetraethyl orthosilicate (TEOS) was added. React for two hours, turn off heating and stirring, and the product is separated by centrifugation, and washed twice with deionized water and absolute ethanol. Then the product was dispersed in 100 mL of ethanol, 1 g of ammonium nitrate was added, and stirred at 80° C. for 2 hours to remove the template agent CTAB. Wash twice with ethanol and several times with water.

Embodiment 2

[0029] The synthesis of embodiment 2 DATS-MSN

[0030] Take 10 mg MSN, ultrasonically disperse it in 2 mL water, add 5 μL DATS, and stir magnetically. After 12 hours, the product is centrifuged, and the supernatant is discarded to obtain drug-loaded particles, namely DATS-MSN.

Embodiment 3

[0031] Embodiment 3 DATS-MSN material characterization

[0032] The size and surface structure of DATS-MSN can be determined by high-resolution transmission electron microscopy (HRTEM, JEM-2100F). The dispersion index (PDI) was obtained by analyzing the particle size distribution with an Autosizer 4700 (Malvern) instrument (25°C, 5 μg / mL). BET (Multipoint Brunauer, Emmett, and Teller) analysis was used to calculate the surface area-to-mass ratio of MSN.

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Abstract

The invention relates to a controlled-release hydrogen sulfide donor. The donor is obtained by reacting diallyl trisulfide with an ordered mesoporous silicon dioxide nanoparticle materials. The invention also provides a preparation method and application of the hydrogen sulfide donor. The controlled-release hydrogen sulfide donor is capable of slowly releasing medium-concentration hydrogen sulfide, can be regulated by use of glutathione and has no obvious cytotoxicity; as a result, the controlled-release hydrogen sulfide donor is an ideal hydrogen sulfide donor for in-vivo and in-vitro experiments and clinical application.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a slow-release hydrogen sulfide donor and its preparation method and application. Background technique [0002] With the discovery of hydrogen sulfide as an endogenous metabolite, domestic and foreign scholars have conducted a lot of research on the physiological significance of hydrogen sulfide and its important role in various diseases in the past 10 years. A large number of studies have shown that the reduction of hydrogen sulfide level is involved in the pathophysiological process of myocardial ischemia-reperfusion injury. It can also cause coronary artery dilation through the synergistic effect of nitric oxide, thereby increasing coronary blood flow, improving coronary microcirculation after reperfusion, further reducing myocardial ischemia / reperfusion injury, promoting the recovery of cardiac function, and reducing myocardial Apoptosis of cells. However, the cur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K33/04A61K33/00A61P9/08A61P9/10A61P9/00A61K31/095
CPCA61K31/095A61K33/04A61K38/063A61K47/6923C01B17/16A61K2300/00
Inventor 过常发王春生孙笑天张绘莉杨武利朱铠王文硕
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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