Construction method of HPLC (High Performance Liquid Chromatography) fingerprint spectrum of salvia miltiorrhiza and radix puerariae depression relieving drug

Abstract

The invention provides a construction method of an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of a salvia miltiorrhiza and radix puerariae depression relieving drug. The method comprises the steps of preparing a sample solution, preparing a reference substance solution, preparing efficient liquid chromatogram condition, and detecting to obtain the HPLC fingerprint spectrum of the salvia miltiorrhiza and radix puerariae depression relieving drug; 17 characteristic peaks are detected from the HPLC fingerprint spectrum of the salvia miltiorrhiza and radix puerariae depression relieving drug, including 14 characteristic peaks of salvia miltiorrhiza, and 3 characteristic peaks of radix puerariae. According to the construction method, the HPLC-DAD-TOF / MS serial connecting technology is carried out to point out each chromatographic peak of the HPLC fingerprint spectrum of the salvia miltiorrhiza and radix puerariae depression relieving drug; meanwhile, the one-testing and multi-evaluation method is carried out to detect the content of main effective ingredients of the salvia miltiorrhiza and radix puerariae depression relieving drug, as well as detecting the content limit standard; therefore, the construction method of the fingerprint spectrum of the salvia miltiorrhiza and radix puerariae depression relieving drug has the advantages that the qualitative perforamnce and quantification performance are combined to construct a multi-information fingerprint spectrum with clear spectrum, clear ingredients and controllable content, thus the quality of the salvia miltiorrhiza and radix puerariae depression relieving drug can be comprehensively reflected, and the quality control method of the salvia miltiorrhiza and radix puerariae depression relieving drug can be effectively improved.

Description

technical field [0001] The invention relates to the technical field of drug analysis, in particular to a method for constructing the HPLC (high performance liquid chromatography) fingerprint of Shenge Shuxin Prescription. Background technique [0002] Traditional Chinese medicine fingerprint is a method widely accepted at home and abroad to comprehensively evaluate the quality mode of traditional Chinese medicine. It is an effective control method for the overall evaluation of the quality of traditional Chinese medicine in complex samples with multidimensional components. The US FDA allows the declaration in the guidelines for botanical pharmaceutical products The author provides the chromatographic fingerprint information of the product, and the German Society of Medicinal Botany, the British Herbal Codex, the Indian Herbal Pharmacopoeia, and the Canadian Association of Medicinal Aromatic Plants also use fingerprints as one of the quality control standards. [0003] Shenge ...

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Problems solved by technology

[0004] The applicant has previously carried out patent protection for the preparation method and preparation of the compound and its effective chemical components (a preparation method and preparation of traditional Chinese medicine for the treatment of coronary heart disease and its effective chemical components, patent number 201110330186.3), due to the compound The quality control method is not perfect, it is difficult to comprehensively control the quality of the compound formula and the curative effect of the drug, and it is somewhat one-sided to use the activity of one of the drugs to explain the quality of Shenge Shuxin Prescription

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