Process for preparing viable bacillus subtilis preparation

Abstract

The invention relates to a process for preparing a viable bacillus subtilis preparation. According to the process, after bacillus subtilis undergoes first-stage germ amplification culture and second-stage germ amplification culture, the bacillus subtilis is inoculated to a fermentation culture medium for culture, and the number and conversion ratio of bacilli is increased through feeding a supplement carbon source during fermentation, so that the unit yield is increased remarkably, and the unit cost is reduced; at the end of fermentation, fermentation liquor is concentrated with a vacuum film concentrator, so that soluble synergistic substances, growth promoting substances and bacteriostatic active substances can be effectively recovered from the fermentation liquor; the concentrated fermentation liquor is subjected to drying and granulating by multi-level low-temperature spray-fluidized drying equipment, granules are subjected to sorting, fine powder gathered by a cyclone separator returns and is granulated again, and then, a bacillus subtilis product, of which the CFU of powder per gram is not less than 2.0*10<11>, is obtained; exhaust gas is treated and is discharged after reaching standard. The process has the advantages that effective substances in the fermentation liquor can be reserved to the maximum, the quality of the product is high, the equipment utilization ratio is high, the retention of biological activity is relatively good, waste gas and waste water are discharged after reaching standard, and large-scale industrial production can be achieved.

Description

technical field [0001] The invention relates to a preparation process of a live bacillus subtilis preparation. Background technique [0002] Bacillus subtilis (Bacillus subtilis), is a kind of Bacillus genus. Single cell 0.7~0.8×2~3 microns, uniform coloring. No capsule, perinatal flagella, able to move. Gram-positive bacteria, spore 0.6 ~0.9×1.0~1.5 microns, oval to columnar, located in the center of the cell or slightly biased, the cell does not expand after spore formation. The surface of the colony is rough and opaque, stained white or slightly yellow, and often forms wrinkles when growing in liquid medium It is an aerobic bacterium. It can use protein, various sugars and starch to decompose tryptophan to form indole. It is widely used in genetic research, and the synthesis pathway and regulation mechanism of purine nucleotides in this bacterium are relatively clear. Widely distributed in soil and decaying organic matter, it is easy to reproduce in withered grass juice,...

AI Technical Summary

Problems solved by technology

[0005] 1. The filling coefficient of the fermentation tank is 60%-70%, and the utilization rate of the equipment is not high;

[0006] 2. Due to the batch fermentation adopted, the initial concentration of the fermentation medium is too high, and the lag period after inoculation is longer.

[0007] During the fermentation process, more by-products are formed, and the concentration of fermentation products cannot increase, which eventually leads to higher fermentation costs;

[0008] 3. Flocculation / plate-and-frame filtration or centrifugation technology is generally used for the concentration of fermentation broth. These two technologies generally generate a large amount of wastewater, and the subsequent treatment costs are relatively high;

[0009] 4. The use of flocculation / plate-and-frame filtration or centrifugation technology will also cause the loss of active ingredients in the fermentation supernatant, resulting in a decline in the product effect;

[0010] 5. The inlet temperature of spray drying is generally set at 180°C to 200°C, and the outlet temperature is 80°C to 100°C. Under such a high working temperature, the number of bacteria in some varieties will decrease to a certain extent, which directly affects product quality

[0013] Fermentation and drying and granulation will produce a large amount of waste gas, which often contains volatile organic compounds (VOC), hydrogen sulfide, ammonia, mercaptans and other pollutants, accompanied by unpleasant stench. In the past, fermentation enterprises generally Discharge it directly or simply spray it with water, the two treatment methods will cause serious pollution to the environment