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Modified PAMAM (polyamide-amine) dendrimer based double-crosslinked in-situ gel and preparation method thereof

An in-situ gel and double cross-linking technology, applied in the field of biomedical tissue engineering materials, achieves the effects of convenient preparation, mild reaction conditions and good biocompatibility

Inactive Publication Date: 2014-12-24
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The in situ gel and its preparation method have not been reported yet, and it has good application prospects in the fields of bioadhesion, tissue engineering, etc.

Method used

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  • Modified PAMAM (polyamide-amine) dendrimer based double-crosslinked in-situ gel and preparation method thereof
  • Modified PAMAM (polyamide-amine) dendrimer based double-crosslinked in-situ gel and preparation method thereof
  • Modified PAMAM (polyamide-amine) dendrimer based double-crosslinked in-situ gel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Weigh 600mg of the fourth-generation polyamidoamine (G4-PAMAM), dissolve it in 25ml of DMF, add 0.4g of HBTU, 125mg of HOBT, 150mg of NAC and 0.3ml of DIPEA, keep away from light at room temperature, keep stirring at a stirring rate of 200rpm for 24h, and add enough The amount of cold ether precipitation. The product was dissolved in deionized water, dialyzed in saturated sodium bicarbonate solution and deionized water for 48 hours respectively to remove unreacted amino acids and coupling reagents, and freeze-dried at -40°C to obtain G4-PAMAM- sh. Dissolve 20 mg of the sample in 100 μl of phosphate buffer solution with pH=7.2~7.4, and stir until completely dissolved.

[0026] Weigh 2g of dextran powder with a number-average molecular weight of 70,000 and dissolve it in 80ml of deionized water, stir at room temperature until completely dissolved; weigh 1.6g of NaIO 4 The powder was dissolved in 25ml of deionized water, stirred until completely dissolved; the NaIO 4 Th...

Embodiment 2

[0029] Weigh 600mg of third-generation polyamidoamine (G3-PAMAM), dissolve it in 20ml of DMF, add 1g of HBTU, 0.25g of HOBT, 0.3g of NAC and 0.6ml of DIPEA, keep away from light at room temperature, and keep stirring at a stirring rate of 200rpm for 24h. After the reaction, add enough Cold ether precipitation. The product was dissolved in deionized water, dialyzed in saturated sodium bicarbonate solution and deionized water for 48 hours respectively to remove unreacted amino acids and coupling reagents, and freeze-dried at -40°C to obtain G3-PAMAM- sh. Dissolve 20 mg of the sample in 100 μl of phosphate buffer solution with pH=7.2~7.4, and stir until completely dissolved.

[0030] Weigh 1g of dextran powder with a number average molecular weight of 70,000 and dissolve it in 80ml of deionized water, stir at room temperature until completely dissolved; weigh 0.8g of NaIO 4 The powder was dissolved in 25ml of deionized water, stirred until completely dissolved; the NaIO 4 The so...

Embodiment 3

[0033] Weigh 600mg of the fourth-generation polyamidoamine (G4-PAMAM), dissolve it in 25ml of DMF, add 0.6g of HBTU, 200mg of HOBT, 300mg of NAC and 0.4ml of DIPEA. The amount of cold ether precipitation. The product was dissolved in deionized water, dialyzed in saturated sodium bicarbonate solution and deionized water for 48 hours to remove unreacted amino acids and coupling reagents, and freeze-dried at -40°C to obtain G4-PAMAM- sh. Dissolve 20 mg of the sample in 100 μl of phosphate buffer solution with pH=7.2~7.4, and stir until completely dissolved.

[0034] Weigh 3g of dextran powder with a number average molecular weight of 70,000 and dissolve it in 80ml of deionized water, stir at room temperature until completely dissolved; weigh 2.4g of NaIO 4 The powder was dissolved in 25ml of deionized water, stirred until completely dissolved; the NaIO 4 The solution was added into the dextran solution, protected from light, and continued to stir for 24h with a stirring rate o...

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Abstract

The invention discloses a modified PAMAM (polyamide-amine) dendrimer based double-crosslinked in-situ gel and a preparation method thereof. The gel consists of the following components in percentage by weight: 2-10% of glucan aldehyde, 5-15% of thiol PAMAM and the balance of water. A preparation process comprises the following steps: preparing a thiol modified PAMAM dendrimer; preparing a thiol modified PAMAM dendrimer solution; preparing a glucan aldehyde solution; and mixing the thiol modified PAMAM dendrimer solution and the glucan aldehyde solution to prepare the double-crosslinked in-situ gel. The dendrimer in the gel disclosed by the invention has the advantages of highly-intensive active crosslinking groups and controllable surface functional group density, and is used for constructing the gel by virtue of in-situ crosslinking of strong and weak chemical bonds to ensure that the gel shows relatively high storage modulus and strength. Moreover, the preparation method disclosed by the invention does not need any small-molecular additives and illumination operations, is simple, convenient and feasible, is good in biocompatibility, and can be applied to the fields of biological adhesion, tissue engineering and the like.

Description

technical field [0001] The invention relates to a double-crosslinked in-situ gel based on modified PAMAM dendrons and a preparation method thereof, belonging to the technical field of biomedical tissue engineering materials. Background technique [0002] In situ hydrogels, especially natural materials such as polysaccharides, have low storage modulus and poor mechanical properties, which severely limit their wide application. Dendrimers are introduced into natural material in situ gels as component raw materials. It is expected to enhance its mechanical strength. Dendritic macromolecules often lead to a series of branched molecular chains from one molecule, and have a regular hyperbranched structure. Polyamide-amine (PAMAM) is the most common dendritic material, and the molecule contains a large number of amide bonds. commoditization. The PAMAM structure is highly symmetrical and has the characteristics of highly intensive surface amino groups. When cross-linked into a gel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08J3/075C08L87/00C08L5/02C08G83/00C08B37/02
Inventor 赵瑾宋利锋袁晓燕
Owner TIANJIN UNIV
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