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Callicarpa nudiflora medicine, intermediate and fingerprint detection method and standard fingerprint of preparation

A technology of standard fingerprint spectrum and naked flower violet bead, applied in the field of fingerprint spectrum detection of naked flower violet bead, intermediates and their preparations, and can solve the problems of unavailability, effective control of product quality, and inability to better guarantee clinical efficacy.

Active Publication Date: 2014-12-17
JIUZHITANG +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no good quality control method in the prior art to accurately reflect the quality status of existing Chinese medicinal materials, intermediates and finished products, and it cannot be used in the production process and the effective control of product quality, so it cannot be better guaranteed Its clinical efficacy

Method used

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  • Callicarpa nudiflora medicine, intermediate and fingerprint detection method and standard fingerprint of preparation
  • Callicarpa nudiflora medicine, intermediate and fingerprint detection method and standard fingerprint of preparation
  • Callicarpa nudiflora medicine, intermediate and fingerprint detection method and standard fingerprint of preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 Establishment of fingerprints of the medicinal material of Auranthus nudiflora.

[0063] (1) Source of medicinal materials

[0064] In order to make the medicinal materials sufficiently representative, medicinal materials from different origins or different harvest seasons were collected as test samples. See Table 1.

[0065] Table 1 Sources of medicinal materials of Zizhu nudiflora

[0066]

[0067] (2) Instruments and reagents

[0068]Agilent 1200 HPLC, ChemStation chromatography workstation; column: ZORBAX SB C 18 Column (4.6mm×250mm, 5μm, Agilent Corporation, USA); KQ 3200 DE CNC ultrasonic cleaner (Kunshan Instrument Co., Ltd.); Milli-Q pure water system (Millipore Corporation, USA); BS110S electronic balance (Beijing Sartorius Balance Co., Ltd.).

[0069] (3) Selection of chromatographic conditions

[0070] ①Selection of mobile phase

[0071] Select acetonitrile-water, acetonitrile-0.1% phosphoric acid solution, acetonitrile-0.4% phosphoric aci...

Embodiment 2

[0104] Example 2 Establishment of the fingerprint of the intermediate of Auranthus nudiflora.

[0105] (1) Instruments and reagents

[0106] Agilent 1200 HPLC, ChemStation chromatography workstation; column: ZORBAX SB C 18 Column (4.6mm×250mm, 5μm, Agilent Corporation, USA); KQ 3200 DE CNC ultrasonic cleaner (Kunshan Instrument Co., Ltd.); Milli-Q pure water system (Millipore Corporation, USA); BS110S electronic balance (Beijing Sartorius Balance Co., Ltd.).

[0107] (2) Chromatographic conditions

[0108] The chromatographic column is a C18 reverse-phase chromatographic column; the column temperature is 30°C; the detection wavelength is 327nm; the mobile phase A is acetonitrile, the mobile phase B is 0.4% phosphoric acid solution, and the total flow rate is 1.0ml / min; the analysis time is 25 minutes;

[0109] Table 4 Gradient running table

[0110]

[0111] (3) Preparation of reference solution

[0112] Weigh the reference substance of isoverbacoside, place it in a vo...

Embodiment 3

[0129] Example 3 Establishment of fingerprints of the Nudehuazizhu preparation (taking Nudehuazizhu Tablets as an example).

[0130] (1) Instruments and reagents

[0131] Agilent 1200 high performance liquid chromatograph, ChemStation chromatographic workstation; Chromatographic column: ZORBAX SB C18 column (4.6mm × 250mm, 5μm, American Agilent Company); KQ 3200 DE type numerical control ultrasonic cleaner (Kunshan Instrument Co., Ltd.); Milli- Q pure water system (Millipore, USA); BS110S electronic balance (Beijing Sartorius Balance Co., Ltd.).

[0132] (2) Chromatographic conditions

[0133] The chromatographic column is a C18 reverse-phase chromatographic column; the column temperature is 30°C; the detection wavelength is 327nm; the mobile phase A is acetonitrile, the mobile phase B is 0.4% phosphoric acid solution, and the total flow rate is 1.0ml / min; the analysis time is 25 minutes;

[0134] Table 6 Gradient running table

[0135]

[0136] (3) Preparation of refere...

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Abstract

The invention discloses a callicarpa nudiflora medicine, an intermediate and a fingerprint detection method of a preparation. The method comprises the following steps: (a) preparation of a reference solution; (b) preparation of a test solution; (c) chromatographic condition; (d) formulation of a standard fingerprint of isoacteoside as a reference peak; and (e) quality control of the fingerprint. According to the above method, a pretreatment method of each tested object is simple, characteristic constituents are retained completely, stability is good, precision is high, and there is certain specificity. The separation effect of each characteristic peak in the fingerprint is good. The callicarpa nudiflora medicine, the intermediate and the fingerprint of the preparation have good correlation. The method can be used for identifying quality of the callicarpa nudiflora medicine and increasing production process controllability of the callicarpa nudiflora preparation, and is helpful for guaranteeing quality stability and clinical efficacy of the callicarpa nudiflora preparation. The invention also discloses the callicarpa nudiflora medicine obtained according to the above fingerprint detection method, the intermediate and the standard fingerprint of the preparation.

Description

technical field [0001] The invention belongs to the technical field of drug quality control, and in particular relates to a method for detecting fingerprints of Auranthus nudiflora, intermediates and preparations thereof, and obtains standard fingerprints thereof. Background technique [0002] Callicarpa nudiflora Hook.et Arn. is the dry leaf of Verbenaceae plant. Distributed in Guangdong, Guangxi, Hainan and other places, it grows at an altitude of 1200 meters. The main components of Nadihua Zizhu are flavonoid glycosides, condensed tannins, neutral resins, phenols, polysaccharides, hydroxyl compounds, magnesium, calcium, and iron salts. Modern studies have shown that its active ingredients have hemostatic, antibacterial, astringent, detoxifying, analgesic, and healing-promoting effects. Its antibacterial and antiviral effects, especially have different inhibitory effects on Staphylococcus aureus, typhoid bacillus, and pneumococcus, among which the antibacterial effect on...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 卢凤来张妮瑜谷陟欣辛秀李典鹏袁莉黄胜颜冬兰朱丽颜小捷陈诗圣
Owner JIUZHITANG
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