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Paper chip detection method for rapidly determining content of amylose in paddy rice and application system adopted in paper chip detection system for rapidly determining content of amylose in paddy rice

A technology for rapid determination of amylose content, which is applied in the direction of analysis by chemical reaction of materials, and material analysis by observing the influence of chemical indicators. And popular, not easy to preserve, not easy to volatilize effect

Inactive Publication Date: 2014-12-17
CHINA NAT RICE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these analytical methods require specific expensive instruments and equipment, and need to be carried out in a specific laboratory, which is not easy to promote in ordinary laboratories.

Method used

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  • Paper chip detection method for rapidly determining content of amylose in paddy rice and application system adopted in paper chip detection system for rapidly determining content of amylose in paddy rice
  • Paper chip detection method for rapidly determining content of amylose in paddy rice and application system adopted in paper chip detection system for rapidly determining content of amylose in paddy rice
  • Paper chip detection method for rapidly determining content of amylose in paddy rice and application system adopted in paper chip detection system for rapidly determining content of amylose in paddy rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1, prepare the paper chip detection system of rapid determination of amylose content in rice:

[0075] The paper chip detection system comprises a potassium iodide paper chip (that is, a paper chip regioselectively adsorbed with potassium iodide) and a chromogenic solution;

[0076] 1. The manufacturing method of the potassium iodide paper chip substrate is as follows:

[0077] ①, using test paper (conventional test paper) as the paper base, using conventional methods (such as hexadecyl trichlorosilane chemical modification combined with vacuum plasma treatment technology, wax printing technology, hexadecyl trichlorosilane chemical modification combined Ultraviolet light degradation technology, hexadecyl trichlorosilane chemical modification combined with corona treatment technology, etc.) set up a hydrophilic channel network on the test paper --- a rice-shaped hydrophilic channel network.

[0078] The rice-shaped hydrophilic channel network includes a centr...

Embodiment 2

[0084] Embodiment 2, adopt the paper chip detection method of the rapid determination of amylose content in rice carried out by the detection system obtained in Example 1:

[0085] 1) According to the standard NY / T 55-1987 of the Ministry of Agriculture, prepare the rice sample solution to be tested, the rice amylose standard sample solution and the blank sample solution, and double the amount of the sample weighed during the preparation process.

[0086] Carry out three parallel determinations of the same rice sample to be tested or three unknown samples at the same time; set up 4 different rice amylose standard sample solutions with known amylose content: these 4 standard samples with known amylose content : 1.5%, 10.4%, 16.2%, 26.4%.

[0087] That is, specifically:

[0088] Weigh 100mg±0.2mg of the rice sample / rice amylose standard sample to be tested, add 0.5mL ethanol with a volume concentration of 95% to make the sample wet and disperse; then add 4.5mL 1mol / L NaOH solut...

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Abstract

The invention discloses a paper chip detection system for rapidly determining the content of amylose in paddy rice. The paper chip detection system comprises a potassium iodide paper chip and a developing solution; a production method of the potassium iodide paper chip comprises the steps: arranging a hydrophilic channel network on a paper substrate, wherein the hydrophilic channel network comprises a central area and a channel surrounding the central area, and the part of the paper substrate, except for the hydrophilic channel network, is hydrophobic; dropwise adding a KI-Na2CO3 aqueous solution in the central area, and naturally permeating the KI-Na2CO3 aqueous solution in the hydrophilic channel network; and naturally drying the paper substrate to obtain the potassium iodide paper chip. The developing solution is an acid-strong oxidant aqueous solution. The invention also provides a detection method adopting the paper chip detection system. The detection method comprises the following steps of preparing a to-be-detected paddy rice sample solution, a paddy rice amylose standard sample solution, a blank sample solution and the like. By adopting the paper chip detection method and the paper chip detection system, the content of the amylose in the paddy rice can be rapidly and conveniently determined.

Description

technical field [0001] The invention relates to the field of food detection, in particular to a paper chip detection method for rapidly detecting amylose content in rice and a detection system used therein. Background technique [0002] Starch is a natural polysaccharide, the main component of grains, and has been widely used in various food and non-food industries. The properties of starch are critical to its end use. The content of amylose in starch is closely related to the gelatinization, gelatinization, viscosity, solubility, swelling ability, digestibility and resistance of starch. Therefore, amylose content is an important index to evaluate the sensory quality and processing quality of grains. In addition, amylopectin and amylopectin ratio are important factors affecting the formation of resistant starch. The amylose content in rice is an important index in the routine detection of rice quality. Therefore, the determination of amylose content in rice has very impo...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 胡贤巧朱智伟方长云卢林段彬伍张婷婷
Owner CHINA NAT RICE RES INST
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