Paper chip detection method for rapidly determining content of amylose in paddy rice and application system adopted in paper chip detection system for rapidly determining content of amylose in paddy rice
A technology for rapid determination of amylose content, which is applied in the direction of analysis by chemical reaction of materials, and material analysis by observing the influence of chemical indicators. And popular, not easy to preserve, not easy to volatilize effect
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Embodiment 1
[0074] Embodiment 1, prepare the paper chip detection system of rapid determination of amylose content in rice:
[0075] The paper chip detection system comprises a potassium iodide paper chip (that is, a paper chip regioselectively adsorbed with potassium iodide) and a chromogenic solution;
[0076] 1. The manufacturing method of the potassium iodide paper chip substrate is as follows:
[0077] ①, using test paper (conventional test paper) as the paper base, using conventional methods (such as hexadecyl trichlorosilane chemical modification combined with vacuum plasma treatment technology, wax printing technology, hexadecyl trichlorosilane chemical modification combined Ultraviolet light degradation technology, hexadecyl trichlorosilane chemical modification combined with corona treatment technology, etc.) set up a hydrophilic channel network on the test paper --- a rice-shaped hydrophilic channel network.
[0078] The rice-shaped hydrophilic channel network includes a centr...
Embodiment 2
[0084] Embodiment 2, adopt the paper chip detection method of the rapid determination of amylose content in rice carried out by the detection system obtained in Example 1:
[0085] 1) According to the standard NY / T 55-1987 of the Ministry of Agriculture, prepare the rice sample solution to be tested, the rice amylose standard sample solution and the blank sample solution, and double the amount of the sample weighed during the preparation process.
[0086] Carry out three parallel determinations of the same rice sample to be tested or three unknown samples at the same time; set up 4 different rice amylose standard sample solutions with known amylose content: these 4 standard samples with known amylose content : 1.5%, 10.4%, 16.2%, 26.4%.
[0087] That is, specifically:
[0088] Weigh 100mg±0.2mg of the rice sample / rice amylose standard sample to be tested, add 0.5mL ethanol with a volume concentration of 95% to make the sample wet and disperse; then add 4.5mL 1mol / L NaOH solut...
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