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Chromatographic separation purification method of paeonol

A technique for separation and purification of paeonol, which is applied in the field of chromatographic separation and purification of paeonol to achieve the effects of less consumption, reduced decomposition and inhibition of allergic reactions

Inactive Publication Date: 2014-12-17
洛阳莱珂麝薇化妆品有限公司
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  • Abstract
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Problems solved by technology

[0004] The main purpose of the present invention is to overcome the defects of existing paeonol purification methods and provide a new chromatographic separation and purification method for paeonol. The technical problem to be solved is to use the least paeonol consumption , to separate and purify Paeonol, which has the most analgesic, anti-inflammatory, antipyretic and anti-allergic effects, which is very suitable for practical use

Method used

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Embodiment Construction

[0031] In order to further elaborate the technical means and effects that the present invention adopts to achieve the intended purpose of the invention, the specific implementation, method steps, Features and their functions are described in detail below.

[0032] A kind of chromatographic separation and purification method of paeonol of the present invention, the reagent that it uses comprises:

[0033] Reagents for column chromatography: PBS and K 2 HPO 4 Mixtures of different concentrations were made, and homemade hydroxyapatite (O.4M Na 2 HPO 4 +0.4M CaCl 2 ); DEAE-Sepharose Fast Flow, CM-Sepharose Fast Flow, Sephadex G-25 and Sephadex G-200.

[0034] Denaturing electrophoresis reagents:

[0035] 30% Acr-Bis; 30% Acr, 1% Bis;

[0036] 4×Separating gel buffer: 1.5M Tris-Hcl (pH8.9), 0.4% SDS, pH8.9;

[0037] 4x stacking gel buffer: 0.5M Tris-HCl (pH6.7), 0.4% SDS, pH6.7;

[0038] 1% TEMED;

[0039] 10% AP: 0.19 AP, 1ml H 2 O (now equipped):

[0040] Electrophore...

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Abstract

The invention is a chromatographic separation purification method of paeonol. The method comprises the following steps: cell disruption, salting out, dialysis, selection column chromatography, column packing, sample loading on the column and elution drying. The technical scheme of the invention reduces the part of disappeared paeonol due to dissolving in hot steam in a traditional distillation method, also reaches temperature control of paeonol in the separation process, and effectively reduces decomposition of the organic matter paeonol in high temperature, so as to achieve separation and purification of the maximum amount of paeonol from the minimum amount of bark of peony. The paeonol has analgesic, anti-inflammatory, antipyretic and anti allergic reaction functions, obvious analgesic effect on pains, obvious inhibitory effect on inflammation, obvious antipyretic effect on body temperature raising, and inhibition effect on allergic reaction of II, III, IV types; and paeonol can inhibit the generation of oxygen free radicals in cells, and whiten the skin.

Description

technical field [0001] The invention relates to a chromatography separation and purification method for paeonol, in particular to a technique for separating paeonol, a component with medicinal value, from the cortex of tree peony by chromatography. Background technique [0002] Peony Paeonia suffruticosa, Paeoniaceae, Paeonia genus, is native to the mountainous areas of Qinling and Daba Mountains in western China. Peony is a unique woody and precious flower in my country. It has large and colorful flowers, elegant and luxurious, rich and dignified, rich fragrance and various varieties. It is known as "national beauty and heavenly fragrance" and "king of flowers". It has long been regarded as A symbol of wealth, auspiciousness, and prosperity. Luoyang peony is the most famous. In addition to its ornamental value, the processed peony root "Paeonol" is an important medicinal material, which enters the heart, liver, and kidney meridians, and has the functions of clearing away h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C49/84C07C45/79C07C45/78A61P29/00A61Q19/02A61Q19/00
CPCC07C45/786A61Q19/02C07C45/79C07C49/84
Inventor 石泽宇
Owner 洛阳莱珂麝薇化妆品有限公司
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