Constant-temperature control device and method for reaction liquid in cuvette of full-automatic biochemical analyzer

Abstract

The invention discloses a temperature control device and method for reaction liquid in a cuvette of a full-automatic biochemical analyzer. In the device, sensors for directly measuring the reaction liquid temperature Treal, the incubation tray temperature Tcontrol and the ambient temperature Tenviron in the cuvette are arranged respectively, a single chip microcomputer is used for analyzing, computing and processing temperature data acquired by these sensors, and the output power of a heater is controlled through a voltage-controlled driving circuit to heat an incubation tray. According to the method, the reaction liquid temperature Treal in the cuvette reaches a target temperature and is kept in a constant-temperature heat balance state through a first dynamic balance control process and a second dynamic heat balance control process. Through adoption of the method, the influences of reaction tray rotation, cuvette cleaning and environmental changes (comprising air flow, temperature-humidity and the like) on the constant temperature of the reaction liquid are avoided. The method has the characteristics of high control accuracy, stability, reliability, little interference by the external environment and the like.

Description

technical field [0001] The invention relates to a device and method for constant temperature control of a reaction liquid in a fully automatic photoelectric colorimetric analysis instrument, in particular to a constant temperature control method for a reaction liquid in a cuvette of a fully automatic biochemical analyzer. Background technique [0002] When the automatic biochemical analyzer performs serological detection, it is necessary to precisely control the reaction solution mixed with serum and reagents. The change of temperature has a great impact on the accuracy of the measurement results. For enzymes involved in the reaction For the testing items, the impact of temperature changes on the results is particularly obvious. [0003] The cuvettes of the automatic biochemical analyzer are arranged in a ring and fixed on the reaction plate, the reaction liquid is contained in the bottom of the cuvette, the cuvette is located in the annular groove cavity of the incubation p...

AI Technical Summary

Problems solved by technology

Since the cavity formed by the cuvette, the reaction plate and the incubation plate is not completely airtight, the rotation of the reaction plate during work, the heat loss when the cuvette is cleaned, the heat dissipation of the incubation plate, and the change of the ambient temperature will affect the heat balance in the cavity. This effect has the characteristics of nonlinearity, time lag, and uncertainty due to changes in environmental temperature and humidity. The use of conventional temperature regulation controllers cannot achieve the purpose of accurately controlling the temperature of the reaction solution.

[0005] The existing constant temperature control methods for fully automatic biochemical analyzers mostly use the constant temperature control of the temperature of the incubation plate (such as using the temperature sensor of the incubation plate combined with the proportional integral regulator to control the output of the heating element, etc.). However, the temperature of the reaction solution is basically set At 37 degrees Celsius, generally higher than room temperature, there is heat transfer loss between the temperature of the incubation plate and the reaction solution. In order to compensate for this heat loss, it is necessary to adjust the constant control temperature of the incubation plate to slightly higher than 37 degrees. During the production of the instrument During the commissioning process, a precision thermometer is generally used to immerse the probe in the reaction solution. According to the measured temperature of the reaction solution, the constant temperature of the incubation plate is appropriately increased until the temperature of the reaction solution is constant at 37 degrees to measure the heat loss. compensate

[0006] However, since this temperature difference compensation value is only determined for a specific environment during debugging, when the environment in which the instrument is used changes, the temperature difference compensation value will deviate, resulting in errors in temperature control accuracy.

Even if some automatic biochemical analyzers are equipped with an ambient temperature sensor, which can correct the temperature difference compensation value according to the change of the ambient temperature, since the ambient temperature is only one of the factors affecting the thermal balance, the air flow, humidity, and light conditions in the environment , and even the amount of reagents in the reagent tray and the different detection items will affect the thermal balance of the cavity. Therefore, due to the lack of feedback on the direct measurement data of the temperature of the reaction solution, even adding an ambient temperature sensor can only slightly improve the constant temperature control of the reaction solution. Accuracy error, but can not meet the real precise control requirements

Images