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Method for extracting total flavones of hippophae rhamnoides from hippophae rhamnoides leaves

The technology of total flavonoids of sea buckthorn and sea buckthorn leaves is applied in the directions of pharmaceutical formulations, medical preparations containing active ingredients, plant raw materials, etc. The effect of high reusability

Inactive Publication Date: 2014-12-10
NANJING BIAOKE BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many deficiencies in these methods; although the steam distillation method is simple in equipment, low in cost and non-toxic to the environment and human beings, it is suitable for industrial production, but the extraction rate is low and the impurity content is high, so it is rarely used by people now; organic solvent extraction The cost of the method is relatively high, and there is a solvent residual effect, which has a certain impact on humans and the environment; supercritical carbon dioxide extraction technology is a new separation and extraction technology, which has the characteristics of low-temperature extraction, no toxic residual solvents, and selective separation. It is used for the extraction of seabuckthorn flavonoids. The raw materials have to undergo pre-treatments such as degreasing and depigmentation, and the detection process is relatively complicated. Molecular imprinting technology is a new technology developed recently. It can prepare MIP with quercetin as a template. Extraction of flavonoids from the primary extract of seabuckthorn, the experimental results show that MIP using quercetin as a template can effectively extract quercetin and isorhamnetin from the primary extract of seabuckthorn, but the cost of this method is too high and the process is cumbersome , is still in the stage of laboratory exploration
[0004] After searching the literature, there is no literature report on the extraction of total flavonoids from seabuckthorn by combining enzyme fermentation and continuous countercurrent ultrasound

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Dry seabuckthorn leaves at 60°C, crush them, pass through a 40-mesh sieve, take 100g of powder, add 1% of the raw material weight of cellulase and pectinase (the weight ratio is 0.7:0.3, and the enzyme activity unit is 5000U / g). Mix enzymes, stir evenly, and then spray the buffer solution of citric acid / sodium citrate with pH 4.0, so that the raw materials can be held into a ball, and fermented at 40°C for 5 days; the fermented raw materials are placed in a frequency of 40kHz Add 5 times of 60% ethanol solution to a continuous countercurrent ultrasonic extractor, control the temperature at 45°C, extract for 90 minutes, filter to obtain the filtrate; The polyamide resin column with a diameter of 80 meshes was first eluted with distilled water to remove impurities, and then eluted with 4BV of 60% ethanol, the eluate was collected, concentrated under reduced pressure, and dried to constant weight to obtain the total flavonoids extract of seabuckthorn 1.85 g, detected by UV...

Embodiment 2

[0020] Seabuckthorn leaves are dried and crushed at 60°C, passed through a 40-mesh sieve, and 100g of powder is taken, and 1.5% of the raw material weight is added with cellulase and pectinase (the weight ratio is 0.7:0.3, and the enzyme activity unit is 5000U / g) Mix enzymes, stir evenly, and then spray the buffer solution of citric acid / sodium citrate with pH 4.5, so that the raw materials can be held into a ball, and fermented at 45°C for 7 days; put the fermented raw materials at a frequency of 40kHz Add 6 times of 70% ethanol solution to the continuous countercurrent ultrasonic extractor, control the temperature at 50°C, extract for 100 minutes, filter to obtain the filtrate; concentrate the filtrate until it has no alcohol smell, and transfer the diameter to height ratio to 1:6, A polyamide resin column with a particle size of 100 meshes, first eluted with distilled water to remove impurities, then eluted with 6BV of 70% ethanol, collected the eluate, concentrated under re...

Embodiment 3

[0022] Seabuckthorn leaves are dried and crushed at 60°C, passed through a 40-mesh sieve, 100g of powder is taken, and 2% of the raw material weight is added with cellulase and pectinase (the weight ratio is 0.7:0.3, and the enzyme activity unit is 5000U / g) Mix enzymes, stir evenly, and then spray the buffer solution of citric acid / sodium citrate with pH 5.0, so that the raw materials can be held into a ball, and fermented at 50°C for 6 days; put the fermented raw materials at a frequency of 40kHz Add 7 times of 80% ethanol solution to the continuous countercurrent ultrasonic extractor, control the temperature at 60°C, extract for 100 minutes, filter to obtain the filtrate; concentrate the filtrate until it has no alcohol smell, and transfer the diameter to height ratio to 1:8, A polyamide resin column with a particle size of 90 mesh, first eluted with distilled water to remove impurities, then eluted with 80% ethanol of 8BV, collected the eluate, concentrated under reduced pre...

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PUM

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Abstract

The invention relates to a method for extracting total flavones of hippophae rhamnoides from hippophae rhamnoides leaves, and particularly relates to a method for extracting total flavones of hippophae rhamnoides by means of synergy of enzyme fermentation and continuous counterflow ultrasound. The method comprises the following steps: a, baking and grinding the hippophae rhamnoides leaves at 60 DEG C, and screening by a 40-mesh screen; b, fermenting by biological enzyme; c, performing continuous counterflow ultrasound assisted extraction; d, passing through a polyamide resin column, eluting by ethanol, collecting eluent, concentrating and drying to obtain the total flavones of hippophae rhamnoides. According to the method, the hippophae rhamnoides leaves are used as raw materials, and the overlaid effect of biological enzyme fermentation and ultrasound is sufficiently used, so that the solubility of total flavones of hippophae rhamnoides can be improved. The method has the advantages of simple process, short extracting time, mild reaction condition and the like, is environmentally friendly, is capable of protecting bioactivity of the total flavones of hippophae rhamnoides from damage, and is easy to realize industrial production.

Description

technical field [0001] The invention relates to a method for extracting total flavonoids of seabuckthorn from seabuckthorn leaves, in particular to a method for extracting total flavonoids of seabuckthorn in cooperation with continuous countercurrent ultrasonic waves through enzyme fermentation. Background technique [0002] Seabuckthorn is a wild berry plant belonging to the genus Hippophaeaceae, also known as vinegar willow, sandy date, and blackthorn. It is recognized as a precious edible and medicinal plant in the world. Studies have shown that the content of flavonoids in seabuckthorn in high altitude areas is relatively high, and the flavonoids in leaves mainly exist in the form of glycosides, mainly including four aglycones such as isorhamnetin, quercetin, kaempferol and myricetin. Its glycoside compounds have a wide range of pharmacological effects such as anti-myocardial ischemia and hypoxia, anti-arrhythmia, lowering serum cholesterol and enhancing immunity. [00...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A61K127/00
Inventor 张金芳杨存
Owner NANJING BIAOKE BIO TECH
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