Mutagenesis method for tetraploid petunia
A petunia and tetraploid technology, which is applied in the field of tetraploid petunia mutagenesis, can solve the problems of expensive instruments, great influence on plant growth and high mortality of seedlings, and achieves the advantages of reducing the use concentration and improving the induction rate. Effect
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[0027] Embodiment 1: a kind of mutagenesis method of tetraploid petunia,
[0028] 1 Materials and methods
[0029] 1.1 Materials Petunia 'Meilin' seeds are from Zhejiang Hongyue Flower Co., Ltd.
[0030] 1.2 Mutagenesis treatment
[0031] (1) Petunia 'Meilin' seeds are sown in plugs, and the matrix in the plugs is peat soil: perlite: pastoral soil mixed in a mass ratio of 6:1:1. When the two cotyledons of the seedlings are fully expanded, the The absorbent cotton is placed between the two cotyledons and is in close contact with the growth point.
[0032](2) Place the tray in the light incubator, set the light intensity to 4000lx, and the temperature is 30°C, and treat it at 8:00 am and 3:00 pm every day, and prepare it with dimethyl sulfoxide (DMSO) with a mass concentration of 1%. Pendimethalin (Pendimethalin) 6-60mg / L and colchicine mass concentration are 0.1%-1% mixed medicinal solution dripped on the absorbent cotton attached between the 2 cotyledons to ensure that the ...
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