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Corbicula fluminea anti-oxidative peptide and preparation method thereof

A technology of antioxidant peptides and clams, applied in the biological field, can solve the problems of low antioxidant peptide activity and insufficient enzymatic hydrolysis of clams, and achieve the effects of simple and controllable enzymatic hydrolysis conditions, convenient operation, and reduced production costs

Inactive Publication Date: 2014-11-05
CHANGSHU INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned technical scheme has insufficient enzymatic hydrolysis, and the activity of the obtained clam antioxidant peptide is not high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033]1. After the quick-frozen river clam meat is thawed and drained under running water, add water at a ratio of 1:4 (w / w) to liquid, and use a low-temperature and ultra-high pressure continuous flow cell crusher to homogenate, adjust the pH to 6.8, and add river clam meat The mass of 1.0% compound protease (neutral protease: flavor protease = 2:1), enzymolysis at 800W microwave power, 55°C for 30min, the enzymolysis is complete, inactivate the enzyme at 100°C for 15min, cool to room temperature, 4000r / min Centrifuge for 20min, remove the supernatant.

[0034] 2. The supernatant obtained in step 1 is filtered through a cup filter with an ultrafiltration membrane cutting molecular weight of 3KD, and the filtrate is taken.

[0035] 3. Pass the filtrate obtained in step 2 through a glass chromatography column equipped with Sephadex G-50, and use distilled water as the eluent at a flow rate of 0.5mL / min. Collect one tube in 5 minutes, each tube is 3mL, collect 400 tubes in tot...

Embodiment 2

[0039] 1. After the quick-frozen river clam meat is thawed and drained under running water, add water at a ratio of 1:4 (w / w) to liquid, and use a low-temperature and ultra-high pressure continuous flow cell crusher to homogenate, adjust the pH to 7.0, and add river clam meat The mass of 1.1% compound protease (neutral protease: flavor protease = 2:1), enzymolysis at 800W microwave power, 58°C for 28min, complete enzymolysis, 18min at 100°C, cooling to room temperature, 4000r / min Centrifuge for 22min and remove the supernatant.

[0040] 2. The supernatant obtained in step 1 is filtered through a cup filter with an ultrafiltration membrane cutting molecular weight of 3KD, and the filtrate is taken.

[0041] 3. Pass the filtrate obtained in step 2 through a glass chromatography column equipped with Sephadex G-50, use distilled water as the eluent, and the flow rate is 0.8mL / min, and collect it in separate tubes through an automatic partial collector. Collect one tube in 5 minut...

Embodiment 3

[0044] 1. After the quick-frozen river clam meat is thawed and drained under running water, add water at a material-to-liquid ratio of 1:4 (w / w), use a low-temperature ultra-high pressure continuous flow cell crusher to homogenate, adjust the pH to 7.2, and add river clam meat The mass of 1.2% compound protease (neutral protease: flavor protease = 2:1), enzymolysis at 800W microwave power, 60°C for 25min, complete enzymolysis, 20min at 100°C, cooling to room temperature, 4000r / min Centrifuge for 25min and remove the supernatant.

[0045] 2. The supernatant obtained in step 1 is filtered through a cup filter with an ultrafiltration membrane cutting molecular weight of 3KD, and the filtrate is taken.

[0046] 3. Pass the filtrate obtained in step 2 through a glass chromatography column equipped with Sephadex G-50, use distilled water as the eluent, and the flow rate is 1mL / min, and collect it in separate tubes through an automatic partial collector, every 5min Collect one tube,...

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PUM

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Abstract

The invention discloses a corbicula fluminea anti-oxidative peptide and a preparation method thereof. The corbicula fluminea anti-oxidative peptide is prepared by deeply developing corbicula fluminea meat with a compound enzyme microwave synergistic controllable enzymolysis technology, is novel and has an anti-oxidative effect. The utilization rate of corbicula fluminea resources is improved, a new path is provided for deep processing of corbicula fluminea, the additional value of the corbicula fluminea is promoted, the income of peasants is increased, and meanwhile, better social and economic benefits can be gained on the aspect of promoting the development of the corbicula fluminea aquaculture industry.

Description

technical field [0001] The invention relates to a river clam antioxidant peptide and a preparation method thereof, belonging to the field of biotechnology. Background technique [0002] River clam, scientific name (corbicula fluminea), also known as yellow clam, golden clam, flat snail, etc., is a bivalve mollusc and one of the important economic shellfish in my country. River clams are rich in protein (crude protein content in dry samples is over 60%), glycogen, essential amino acids, taurine, multivitamins, and minerals with special physiological functions such as calcium, phosphorus, iron, and selenium. It also contains physiologically active substances such as active peptides. [0003] In recent years, the research on extracting antioxidant active peptides from aquatic proteins has also been gradually carried out. For example, peptides with significant antioxidant activity have been extracted from scale fish, mackerel, giant squid skin, white shrimp head and yellowfin t...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/16
Inventor 刘晶晶韩曜平王雪锋戴阳军金林烽
Owner CHANGSHU INSTITUTE OF TECHNOLOGY
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