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Photoresponsive nanostructure film with high visible light transmittance and application thereof

A nano-structure, high-transmittance technology, applied in nanotechnology, methods of supporting/immobilizing microorganisms, tissue cell/virus culture devices, etc., can solve problems such as complex operation process, affecting cell activity, and long operation time. Achieve high efficiency, less cell damage, and easy operation

Inactive Publication Date: 2014-10-08
周婧
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, changing the temperature will affect the activity of the cells, and the operation process is complicated and the operation time required is relatively long

Method used

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  • Photoresponsive nanostructure film with high visible light transmittance and application thereof
  • Photoresponsive nanostructure film with high visible light transmittance and application thereof
  • Photoresponsive nanostructure film with high visible light transmittance and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Uniformly disperse nano-titanium oxide powder with a size of 10nm in water to form a nanoparticle dispersion with a mass fraction of 10%; mix the nanoparticle dispersion with ethanol and organic solvent tetrahydrofuran at a ratio of 0.02:6:1 to form a precursor body solution; the precursor solution was 20μL / cm 2 Evenly drop the concentration on the polystyrene culture vessel, and then dry it at 40°C. After drying, a light-responsive nanostructure film with a grain size of 10nm and a thickness of 20nm can be obtained, and the visible light transmittance is 98%. . After the cells were cultured on the surface of the culture dish for 24 hours, they were irradiated with 360nm light from the bottom, and the cell detachment rate reached 90.5% after 10 minutes.

[0030] Use an inverted biological microscope to observe the cultured cells before and after UV light irradiation, the inverted micrograph is shown in figure 1 and figure 2 The present embodiment that cell counting ...

Embodiment 2

[0033] The nano-gallium nitride powder with a size of 30nm is uniformly dispersed in water to form a nanoparticle dispersion with a mass fraction of 20%; the nanoparticle dispersion is mixed with propanol and organic solvent chloroform in a ratio of 0.06: 9: 3 Make precursor solution; 2 Evenly drop the concentration on the polystyrene culture vessel, and then dry at 90°C. After drying, a light-responsive nanostructure film with a grain size of 30nm and a thickness of 100nm can be obtained, and the visible light transmittance is 89%. . . After the cells were cultured on the surface of the petri dish for 24 hours, they were irradiated with 450nm light from the bottom, and the cell detachment rate reached 92.6% after 30 minutes.

[0034]

Embodiment 3

[0036] The nano-zinc oxide powder with a size of 20nm is uniformly dispersed in water to form a nanoparticle dispersion with a mass fraction of 15%; the nanoparticle dispersion is mixed with methanol and organic solvent methylene chloride in a ratio of 0.04:8:2 into a precursor solution; the precursor solution by 40μL / cm 2 Evenly drop the concentration on the polystyrene culture vessel, and then dry at 70°C. After drying, a light-responsive nanostructure film with a grain size of 20nm and a thickness of 60nm can be obtained, and the visible light transmittance is 93%. . After culturing the cells on the surface of the culture dish for 24 hours, the cells were irradiated with 400nm light from the bottom, and the cell detachment rate reached 89.6% after 20 minutes.

[0037]

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Abstract

Belonging to the field of tissue engineering, the invention in particular relates to a photoresponsive nanostructure film with high visible light transmittance and application thereof. The preparation method of the photoresponsive nanostructure film comprises the steps of: (1) dispersing photoresponsive nano-particles in water uniformly to form a nano-particle dispersion solution with a mass fraction of 10-20%; preparing the nano-particle dispersion solution, alcohol and an organic solvent into a precursor solution in a proportion of 0.02-0.06:6-9:1-3; and (2) adding the precursor solution onto a polystyrene culture vessel dropwise and evenly according to a concentration of 20-52 microL / cm<2>, and then conducting drying at 40-90DEG C, thus obtaining the photoresponsive nanostructure film with a grain size of 10-30nm, a thickness of 20nm-100nm and visible light transmittance of 89%-98%. Compared with the prior art, the method provided by the invention has the characteristics of high cell detachment efficiency, small cell damage, simple operation and wide applicable cell range, etc., thus having very strong practicability.

Description

technical field [0001] The invention belongs to the field of tissue engineering, and in particular relates to a photoresponsive nanostructure thin film with high transmittance of visible light and its application. Background technique [0002] Cell culture (in vitro cell culture) is an essential technology for research in biological related fields. In the process of in vitro cell culture, how to reduce the adverse factors affecting cell behavior during operation, so that cells can adhere better, and reflect the response to drugs or materials from the cell level, which directly affects new drugs and new medical materials. research and development. [0003] Most cells cultured in vitro are adherent cells. Currently, the commonly used material for cell culture vessels is polystyrene, and adherent cells adhere to the surface of polystyrene cell culture vessels through the interaction between extracellular matrix (ECM) proteins and substrates. Relevant studies have found that ...

Claims

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Application Information

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IPC IPC(8): C23C24/00C12M3/04C12N5/00B82Y40/00
CPCC12M23/20C12M25/02
Inventor 周婧王耀捐
Owner 周婧
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