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Monoclonal antibody of brucella omp31 protein and application thereof

A technology of monoclonal antibody and Brucella, applied in the direction of anti-bacterial immunoglobulin, antibody, bacterial antigen components, etc., can solve the problem of affecting the immune effect of attenuated bacterial vaccine strains, affecting the detection of brucellosis and the prevention of vaccine immunity , diagnostic methods can not be identified and other problems, to achieve the effect of accurate identification, good stability, and good specific identification ability

Inactive Publication Date: 2014-10-08
SOUTHERN MEDICAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

If the omp31 gene carried by the M5-90 vaccine strain is completely knocked out, the immune effect of the attenuated vaccine strain will be affected
In addition, because the existing diagnostic methods cannot distinguish between animals immunized by M5-90 and animals naturally infected by wild virus strains, it has seriously affected the detection of brucellosis and the prevention of vaccine immunity.

Method used

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  • Monoclonal antibody of brucella omp31 protein and application thereof
  • Monoclonal antibody of brucella omp31 protein and application thereof
  • Monoclonal antibody of brucella omp31 protein and application thereof

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Embodiment Construction

[0028] Below in conjunction with embodiment, further illustrate the present invention.

[0029] The Brucella M5-90 vaccine strain used in the present invention is purchased from Lanzhou Veterinary Research Institute and is a well-known vaccine strain in the industry.

[0030] Preparation of Brucella Recombinant Membrane Protein omp31

[0031] The constructed recombinant plasmid pET-30a-omp31 (gifted by Professor Chen Chuangfu, Shihezi University, Xinjiang) was transformed into competent BL21. After the recombinant bacteria were shaken to the logarithmic growth phase, IPTG was added at a final concentration of 1mM to induce the expression of the target protein. After the induction, it was sonicated, and it was identified that the target protein was mainly in the precipitate. After the inclusion bodies were washed with 2M urea concentration inclusion body washing solution (1×PBS, 2M urea, 0.5% TritonX-100, 1mmol / L EDTA, pH8.0), the purity of the target protein was over 85%. ...

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Abstract

The invention discloses a brucella omp31 protein epitope and an antibody and application thereof. The sequence of the epitope is shown as GDDASALHTWSDKTKA. The invention further discloses a monoclonal antibody for identifying the epitope as well as the application of the monoclonal antibody. Both the brucella omp31 protein epitope and the monoclonal antibody contribute to diagnosis of brucellosis.

Description

technical field [0001] The invention relates to a brucella omp31 protein epitope and an anti-brucella omp31 protein monoclonal antibody stimulated by the epitope. The present invention also relates to the epitope antibody and the application of the epitope. Background technique [0002] Brucellosis is one of the most prevalent zoonotic diseases in the world. According to the statistics of the World Health Organization, there are 500,000 cases of human brucellosis every year in the world, causing economic losses every year. up to $3 billion. The prevalence of brucellosis in my country is mainly the mixed infection of Brucella melis, which accounts for 80% of the epidemic strains. [0003] The clinical symptoms of brucellosis are complicated, and the pathological damage caused by fever and miscarriage is easy to be confused with some common diseases; it is difficult for doctors to diagnose brucellosis infection based on the history of contact with sick animals, which brings ...

Claims

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Application Information

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IPC IPC(8): C07K7/08C07K16/12C12N5/20G01N33/577G01N33/569A61K39/40A61K39/10A61P31/04C12R1/91
Inventor 王文敬廖卿宇李婷婷黎诚耀
Owner SOUTHERN MEDICAL UNIVERSITY
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