Extraction method for galanthamine from lycoris radiate

Abstract

The invention provides an extraction method for galanthamine from lycoris radiate. The method includes following steps: (1) determination of chromatographic conditions: employing a Kromasil100-5C18 reversed-phase column (250mm*4.6mm, 5[mu]m) as an chromatographic column and an acetonitrile-phosphoric acid buffer solution (17:83) mixture as an mobile phase, and setting a column temperature to be 35 DEG C, a sample volume to be 20[mu]L and a detection wavelength to be 290nm, wherein the phosphoric acid buffer solution, which is 7.02 in pH value, is prepared by following steps: dissolving 2.72g of potassium dihydrogen phosphate in 100ml of water, adding 1.4ml of triethylamine and performing a diluting process to 1L; (2) establishment of a standard curve: establishing a regression equation according to the chromatographic conditions in the step (1); and (3) extraction of the galanthamine: weighing 2.0g of lycoris radiate. Herb powder, adding cellulase and pectinase, adding an aqueous solution with a pH value being 4.5 at a certain temperature for carrying out an extraction process for a certain time, performing a 5000r / min centrifuge process for 20 min, taking a supernatant, performing chromatographic measurement and calculating a yield of the galanthamine. The invention is low in extraction cost, is short in extraction time, is simple in technology, is free from polluting environment and is high in extraction yield.

Description

Technical field [0001] The invention relates to a method for extracting galantamine, in particular to a method for extracting galantamine from Lycoris radiata. Background technique [0002] Lycoris radiate. Herb is a perennial herb of the Lycoris genus (Amaryllidaceae). It is mainly distributed in Asia. There are 17 species in my country. They are found in Hunan, Hubei, Jiangsu, Zhejiang, Fujian and other provinces. There is distribution. Lycoris plants have high ornamental value and are known as "Chinese Tulips." Lycoris bulbs are rich in starch. If they are ground, washed with plenty of water, and eaten after detoxification, they taste like glutinous rice flour. It is recorded in Materia Medica that it can be fried and immersed in water. Lycoris radiata is pungent, sweet and warm in nature, and has the effect of reducing swelling and killing insects. Its rich alkaloids have also been widely used, especially galantamine. With the serious aging of the population, the Alzheimer's...

AI Technical Summary

Problems solved by technology

Lycoris is one of the plant sources of galantamine raw material medicine, but its content is low, and its extraction usually adopts the solvent extraction method, which is time-consuming, wastes resources, and seriously pollutes the environment. Therefore, a new galantamine is provided. The extraction method of tamin is particularly important

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