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A method for comprehensively extracting multiple biologically active components from Trichosanthes pollen

A bioactive component and comprehensive extraction technology, which is applied in the field of comprehensive extraction of various bioactive components, can solve problems such as the extraction and function of alkaline polysaccharides that have not yet been retrieved, the high price of pure trichosanthin protein, and the waste of trichosanthin resources, so as to improve production Efficiency and Utilization, Ease of Implementation, Effect of Improving Utilization

Inactive Publication Date: 2016-03-23
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the trichosanthin content in Trichosanthes trichosanthes is relatively low, making the price of pure trichosanthin very expensive
When extracting and preparing single components such as trichosanthin and polysaccharides, other bioactive components in trichosanthin are lost in vain. This extraction method not only leads to higher extraction costs, but also wastes trichosanthum resources. Related reports on comprehensive extraction of bioactive components
In addition, the currently extracted trichosanthes polysaccharides are mainly concentrated in water-soluble polysaccharides, and no research on the extraction and function of alkaline polysaccharides has been retrieved.

Method used

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  • A method for comprehensively extracting multiple biologically active components from Trichosanthes pollen
  • A method for comprehensively extracting multiple biologically active components from Trichosanthes pollen
  • A method for comprehensively extracting multiple biologically active components from Trichosanthes pollen

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Effect test

Embodiment 1

[0040] The present invention comprehensively extracts the method for multiple bioactive components from Trichosanthes pollen, and its detailed steps are:

[0041] a. Extraction of alcohol-soluble substances:

[0042] Firstly, weigh 1000g of fresh trichosanthemi powder and grind it, then add ethanol 10 times the weight of trichosanthes pollen (volume percentage concentration of ethanol is 95%), then ultrasonically shake at 25°C for 10 minutes, heat to 90°C and reflux after ultrasonic treatment to extract 4 hour, remove the extract; the extracted extract is suction-filtered, and the obtained filter residue is extracted once again according to the same method above, after extraction, the extract is removed, and after the extract is removed, suction filtration is performed again, and the obtained filter residue is used for further extraction in step b; combined The obtained filtrate was suction filtered twice, and the obtained filtrate was concentrated under reduced pressure (the ...

Embodiment 2

[0083] The present invention comprehensively extracts the method for multiple bioactive components from Trichosanthes pollen, and its detailed steps are:

[0084] a. Extraction of alcohol-soluble substances:

[0085] Firstly, weigh 1000g of fresh trichosanthemi powder and grind it, add ethanol (volume percentage concentration of ethanol is 90%) after crushing, then ultrasonically shake at 25°C for 7 minutes, heat to 90°C after ultrasonic treatment and reflux extraction 3 hour, remove the extract; the extracted extract is suction-filtered, and the obtained filter residue is extracted once again according to the same method above, after extraction, the extract is removed, and after the extract is removed, suction filtration is performed again, and the obtained filter residue is used for further extraction in step b; combined The obtained filtrate was suction filtered twice, and the obtained filtrate was concentrated under reduced pressure (the vacuum degree during the reduced pr...

Embodiment 3

[0097] The present invention comprehensively extracts the method for multiple bioactive components from Trichosanthes pollen, and its detailed steps are:

[0098] a. Extraction of alcohol-soluble substances:

[0099] Firstly, weigh 1000g of fresh trichosanthemi powder and grind it, then add ethanol 5 times the weight of trichosanthes pollen (volume percentage concentration of ethanol is 80%), then ultrasonically shake at 20°C for 5 minutes, heat to 80°C after ultrasonic treatment and reflux to extract 2 hour, remove the extract; the extracted extract is suction-filtered, and the obtained filter residue is extracted once again according to the same method above, after extraction, the extract is removed, and after the extract is removed, suction filtration is performed again, and the obtained filter residue is used for further extraction in step b; combined The obtained filtrate was filtered twice, and the obtained filtrate was concentrated under reduced pressure (the vacuum deg...

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Abstract

The invention discloses a method for comprehensively extracting multiple bioactive components from trichosanthemi pollen. Firstly, ethanol is added to the trichosanthes and ultrasonically oscillated, heated and extracted, the extract is filtered with suction, the filtrate is concentrated and dried to obtain an alcohol soluble product; Polysaccharide; the remaining precipitate is heated and extracted by adding sodium hydroxide solution, the extract is centrifuged, and the supernatant is obtained after treatment to obtain a precipitated alkali-soluble polysaccharide, which is washed and dried to obtain an alkali-soluble crude polysaccharide; the remaining precipitate is then extracted from trichosanthin, extracted Finally, the remaining precipitate is further extracted for trichosanthin agglutinin; finally, the remaining precipitate is washed and dried to obtain trichosanthin starch. The present invention can comprehensively extract alcohol solubles, water-soluble polysaccharides, alkali-soluble polysaccharides, proteins, lectins and starches from the trichosanthum pollen, which utilizes trichosanthum resources to a greater extent and conforms to the current sustainable economic development.

Description

technical field [0001] The invention relates to a method for extracting active ingredients from trichosanthemi, in particular to a method for comprehensively extracting various bioactive ingredients from trichosanthemi. Background technique [0002] Trichosanthes contains a variety of biologically active components, such as trichosanthin, polysaccharides, saponins, lectins, etc. Because the trichosanthin protein has the effects of inducing labor, anti-tumor, anti-virus and anti-HIV; the trichosanthin polysaccharide has activities such as lowering blood sugar and improving immunity. Therefore, at present, there are many studies on the two, and the development and utilization are relatively in-depth, while there are few studies on other bioactive components in trichosanthemi. Existing extraction methods of trichosanthin bioactive substances are mainly based on a single component, such as trichosanthin protein, trichosanthin polysaccharide and trichosanthin lectin. For exampl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C08B30/00C07K14/415C07K14/42C07K1/30
Inventor 陈颖孙涌栋刘明久李勇超
Owner HENAN INST OF SCI & TECH
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