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A detection method and a technology for an area to be detected, applied in the field of detecting ACF
Inactive Publication Date: 2014-09-03
OLYMPUS CORP
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[0013] That is, by analyzing molecules whose expression levels fluctuate at the microlesion stage (i.e., early stage) represented by ACF composed of 50 or less crypts and micro ACFs with a diameter of 1 mm or less, it is possible to capture the future in molecular biology. Colorectal cancer risk and easy assessment not yet achieved
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Embodiment 1
[0087] For all 11 candidate molecules, Met, Cdh1, Ctnnb1, GSTp1, EGFR, iNOS (NOS2), CD44, Fzd1, Ctsb (cathepsin B), PCNA (proliferating cell nuclear antigen), and COX2, the peripheral normal Gene expression levels in tissues and regions suspected of ACF, identifying molecules among these candidate molecule panels whose gene expression levels are specifically upregulated in ACF.
[0088] Specifically, a sample of only an area confirmed as an ACF site under a microscope was collected from a large intestine biopsy sample collected from a patient undergoing lower endoscopy, and a normal tissue collected under a lower endoscope was prepared from the patient. samples, and measure and compare the expression levels of each molecule in each sample. It is shown in more detail below.
[0089] The collected large intestine mucosal tissue of the rectum was observed under a microscope, and only the site confirmed to be the ACF site was excised as an ACF sample. At this time, a commerciall...
Embodiment 2
[0100] According to the results of Example 1, high expression of GSTp1 was observed at both the mRNA level and the protein level in human large intestine ACF. Therefore, using the GSTp1 fluorescent probe, the probe response in surgical resection specimens of human large intestine was studied. The GSTp1 fluorescent probe used in this study is an enzyme activity detection fluorescent probe that has the substrate structure of GSTp1 and produces changes in fluorescence properties through the enzyme reaction with GSTp1, and has fluorescence properties of excitation wavelength 490nm / absorption wavelength 520nm ( Non-Patent Document 9).
[0101] Specifically, after spraying the GSTp1 fluorescent probe solution on the surgical resection specimen of human large intestine, the fluorescence of the ACF lesion was observed under a microscope and an endoscope. More specifically, first, for patients diagnosed with colorectal cancer or ulcerative colitis who underwent extirpation surgery, lo...
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Abstract
Provided is a method for detecting aberrant crypt foci (ACF) by analyzing a region of interest in large intestinal tissue at a molecular level. That is, provided are: a method for detecting ACF using at least one molecule, the expression level of which is elevated specifically to ACF, as an ACF detection marker, and detecting the ACF detection marker in a region of interest in large intestinal tissue, wherein the at least one molecule is selected from the group consisting of Met, Cdh1, Ctnnb1 and GSTp; an ACF detection marker which is a marker for detecting ACF in human-originated large intestinal tissue and is Met, Cdh1, Ctnnb1 or GSTp; and a method for evaluating the risk of colorectal cancer and colorectal adenoma in a human body, which comprises detecting ACF in a region of interest in large intestinal tissue from a subject using the aforementioned ACF detection method and evaluating the risk of colorectal cancer and colorectal adenoma in the subject on the basis of the results of the detection.
Description
technical field [0001] The invention relates to a method for detecting ACF by using the highly expressed molecule of ACF (Aberrant Crypt Focus) as an indicator. [0002] This application claims priority based on Japanese Patent Application No. 2011-285214 for which it applied in Japan on December 27, 2011, and uses the content here. Background technique [0003] Colorectal cancer is the leading cause of death in Japan and the second leading cause of death from cancer in the United States. In the United States, about 150,000 people are newly diagnosed with colorectal cancer every year, and more than 50,000 people die every year (assessed by the American Cancer Society). On the other hand, since it often takes several decades for colorectal cancer to progress from a benign tumor to a malignant tumor, early risk assessment and discovery are expected to contribute to good prognosis and prevention. [0004] As the screening examination methods for colorectal adenomas and tumors...
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