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Method for detecting pharmaceutical preparation for treating xiaoke disease

A technology of pharmaceutical preparations and detection methods, applied in the field of detection of pharmaceutical preparations, can solve the problems of single detection index, unfavorable drug quality, effective control, etc.

Active Publication Date: 2014-08-27
ZHANGZHOU PIEN TZE HUANG PHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For this reason, what the present invention aims to solve is that the existing detection method of the pharmaceutical composition for treating diabetes has the problem that the detection index is single, which is unfavorable for effective control of the quality of the medicine. Targeted method for detecting the pharmaceutical composition for treating diabetes

Method used

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  • Method for detecting pharmaceutical preparation for treating xiaoke disease
  • Method for detecting pharmaceutical preparation for treating xiaoke disease
  • Method for detecting pharmaceutical preparation for treating xiaoke disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] The preparation of embodiment 1 capsule

[0064] [Prescription] Silkworm excrement 7.15kg and licorice 137.5g.

[0065] [Preparation method] For the above two flavors, take silkworm excrement and add 4 times the amount of 60% ethanol, soak overnight, slowly heat to boiling, reflux twice, each time for 1 hour, filter, combine the filtrates, recover ethanol, concentrate to a relative density of 1.01-1.06 (55°C), add 1 times the amount of water and continue to heat until boiling, cool, let stand overnight, take the supernatant, concentrate to a thick paste with a relative density of 1.06-1.15 (measured at 55°C), and set aside. Another licorice was decocted twice with 10 times the amount of water, each time for 2 hours, the filtrate was combined, left overnight to allow precipitation, the supernatant was concentrated to a thick paste with a relative density of 1.04-1.15 (measured at 55°C), and set aside. Combine the two thick pastes, add conventional auxiliary materials ac...

Embodiment 2

[0066] Embodiment 2HPLC method is measured the content of 1-deoxynojirimycin

[0067] 1 Instruments and reagents

[0068] Agilent-1200 high performance liquid chromatography includes: vacuum degasser, quaternary gradient pump, autosampler, diode array detector (DAD), Agilent-Chemistation data processing system (Agilent company); ultrapure water device (MilliQ-Gradient type); ultrasonic generator (Kunshan KQ-300E type).

[0069] 1-deoxynojirimycin reference substance (purchased from SIGMA Company), fluorenylmethoxycarbonyl chloride (purchased from SIGMA Company), acetonitrile was chromatographically pure, water was ultrapure water, and the rest of the reagents were analytically pure.

[0070] 2 Methods and results

[0071] 2.1 Preparation of reference substance solution, test solution and 1-deoxynojirimycin negative sample solution

[0072] 2.1.1 Preparation of reference solution

[0073] Take about 10 mg of 1-deoxynojirimycin reference substance, weigh it accurately, put i...

Embodiment 3

[0141] Embodiment 3HPLC method measures the content of 1-deoxynojirimycin

[0142] Accurately weigh 5 mg of 1-deoxynojirimycin reference substance, dilute with water to make a reference substance stock solution containing 0.1 mg per mL; accurately measure 1 mL of the reference substance stock solution, and accurately add NaHCO with a mass concentration of 1%. 3 Solution 1mL and 1mg / mL fluorenylmethoxycarbonyl chloride acetonitrile solution 1mL, mix well and carry out water bath reaction at 30°C for 20min, then dilute to 20mL with 30% acetonitrile as the reference solution.

[0143] Get the contents of the capsule prepared in Example 1 and grind finely, and accurately weigh 0.5g, add 30mL of water adjusted to pH 3 with concentrated hydrochloric acid, stir evenly, ultrasonically treat for 20min, and centrifuge to get the supernatant; Add an appropriate amount of water with a pH value of 3 to wash, centrifuge to combine the supernatant, and concentrate to 10mL; put the concentrat...

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Abstract

The invention belongs to the field of detection of quality of traditional Chinese preparations, and particularly relates to a method for detecting a pharmaceutical preparation for treating the xiaoke disease. The pharmaceutical preparation comprises the active pharmaceutical ingredients, by weight, 6000-8000 parts of silkworm feces and 100-200 parts of radix glycyrrhizae. According to the method for detecting the pharmaceutical preparation, the pharmaceutical preparation is detected by measuring the content of 1-Deoxynojirimycin through an HPLC method. The method for detecting the pharmaceutical preparation is rapid, comprehensive and highly targeted, the quality of the preparation can be effectively controlled, and use safety and stability of the preparation can be improved.

Description

technical field [0001] The invention belongs to the field of detection of traditional Chinese medicine preparations, and in particular relates to a detection method of a medicinal preparation for treating diabetes. Background technique [0002] At present, there are more than 200 million people with diabetes (diabetes) in the world, and 85% of them are elderly diabetic patients, and the incidence rate is increasing; my country is the country with the largest number of diabetic patients in the world One, second only to the United States. Diabetes is a serious threat to human health and even life. [0003] Chinese patent CN101496829A discloses a pharmaceutical composition for treating diabetes. The raw material of the pharmaceutical composition is mainly composed of silkworm excrement and licorice. The silkworm excrement in the prescription has the effects of expelling wind and dampness, promoting blood circulation and relieving pain. The compound has a good hypoglycemic effe...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/90
Inventor 潘杰于娟吴丽璇陈啟兰
Owner ZHANGZHOU PIEN TZE HUANG PHARM
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