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Isaria cosmopaltriae yasuda polysaccharide and applications thereof in preparing nerve-protective and anti-aging drug

A neuroprotective and anti-aging technology, which is applied in the field of medicine and can solve the problems that there is no refining of Cicada flower extract, and the active functional ingredients are not very clear.

Active Publication Date: 2014-08-20
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the fact that cicadae and the anamorph of the famous and precious Chinese medicine Cordyceps sinensis belong to fungi (Cordyceps genus), and Paecilomyces cicadae has various pharmacological effects such as immunity, and has the advantages of low toxicity and easy cultivation, it is hopeful that it can be used as a substitute for Cordyceps sinensis. Its corresponding active functional components are not very clear, and most of the pharmacological experiments only stay on the basis of the original medicinal materials or crude extracts, and there is no report that the extract of Cicada japonica is further refined to the active site and used to prepare neuroprotective and anti-aging drugs

Method used

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  • Isaria cosmopaltriae yasuda polysaccharide and applications thereof in preparing nerve-protective and anti-aging drug
  • Isaria cosmopaltriae yasuda polysaccharide and applications thereof in preparing nerve-protective and anti-aging drug
  • Isaria cosmopaltriae yasuda polysaccharide and applications thereof in preparing nerve-protective and anti-aging drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0018] Example 2JCH CPS Protective effect on glutamate-induced senescence of PC12 cells

[0019] (1) Determination of cell viability by MTT method and LDH method:

[0020] Take PC12 cells in the logarithmic growth phase (provided by Jiangsu University School of Medicine), and use 2 × 10 4 Inoculate in a 96-well culture plate at 200 μl / L per well. At 37°C, 5% CO 2 After culturing overnight under the condition, the cells were divided into control group, model group and drug treatment group. That is, the control group (complete medium without Glu); model group (complete medium + Glu); drug treatment group (complete medium + JCH CPS +Glu, the concentrations were 200μg / mL, 100μg / mL, and 50μg / mL, diluted with culture medium). JCH CPS After pretreatment for 1 h, add Glu and incubate for 24 h. Set up 4 parallel wells in each group. After culturing for 24 hours, add 20 μl of 5g / L MTT to each well, continue culturing for 4 hours, then terminate the culture, carefully suck the sup...

Embodiment 3

[0039] Example 3JCH CPS In vitro antioxidant activity assay:

[0040] (1) Determination of DPPH free radical scavenging ability:

[0041] Add 1.0mL of 200μmol / DPPH ethanol solution to 1mL of different concentrations of Cicada flower extract solution, then add 2.0mL of 80% ethanol and mix evenly. After placing in a dark place for 30min, measure the absorbance value A at 517nm with a UV spectrophotometer 样品 , and simultaneously measure the absorbance value A of the mixture of 1.0mL DPPH ethanol solution and 3.omL ethanol solution 空白 and the absorbance value A of the mixture of 3mL ethanol and 1.0mL sample 对照 , clearance rate formula: DPPH clearance rate=[A 空白 -(A 样品 -A 对照 ) / A 空白 ] × 100%. The data show that JCH CPS Has the ability to remove DPPH·. The results are shown in Table 5 (2) superoxide anion (O 2·- ) Determination of free radical scavenging ability:

[0042] Add 3ml Tris-HCl (PH8.2) to 1mL different concentrations of Cicada flower extract solution, after 20mi...

Embodiment 4

[0047] The preparation of embodiment 4 dropping pills

[0048] Weigh 400g polyethylene glycol 4000 respectively, melt on a water bath, and then add JCH CPS 450g freeze-dried powder, stir evenly, pour into the insulation tube, adjust the constant temperature device, make the liquid medicine drop into the cooled liquid paraffin at 80-90°C (temperature ± 4°C), after dropping, pour the pills into Drain the paraffin oil on the filter paper, then add a small amount of talcum powder, and mix well to obtain JCH CPS 1000 drop pills.

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Abstract

The invention relates to an isaria cosmopaltriae yasuda polysaccharide and applications thereof in preparing a nerve-protective and anti-aging drug. The isaria cosmopaltriae Yasuda polysaccharide is obtained by adopting the following method: drying isaria cosmopaltriae yasuda materials, grinding and sieving with a pharmacopoeia sieve NO.2, and refluxing and extracting powder twice with water at 90 DEG C, wherein each time lasts for two hours; performing decompression concentration to an extracting liquid, then adding anhydrous ethanol, and settling overnight at 4DEG C-25DEG C; centrifuging for 10-30min at 3000r / min; and drying the settling part to obtain isaria cosmopaltriae yasuda polysaccharide (JCHCPS). The isaria cosmopaltriae yasuda polysaccharide can obviously inhibit aging injury of glutamic acid-induced PC12 cells, stop the release of cells LDH, improve the survival rate of the cells, reduce the level of oxygen radicals in the cells, and improve the activity of glutathione reductase (GSH-Px) and superoxide dismutase (SOD), and shows better antioxidant activity in experiments of clearing DPPH.superoxide anion (O2.-).

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the preparation of parts with neuroprotective and anti-aging activities from Cicada japonica, a Chinese medicinal material, and its application in the preparation of neuroprotective and anti-aging drugs. Background technique [0002] With the aging population in our country, the incidence of neurodegenerative diseases is increasing day by day, and the research on aging and neurodegenerative diseases has become a neuroscience hotspot. The aging of the human body often leads to the accelerated reduction of the number of nerve cells, resulting in some neurodegenerative diseases represented by Alzheimer's Disease (AD). At present, the etiology of neurodegenerative diseases is unknown, and there is no effective treatment. Only a few drugs can be used to treat neurodegenerative diseases. [0003] Cicada is a dry complex formed by the fungus Cordyceps cicadae Shing of the ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P25/00A61P39/06
Inventor 欧阳臻张魏琬麒赵明王吉标尚磊王璠汪愿
Owner JIANGSU UNIV
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