Method of enhancing cadmium absorption of black nightshade from soil by utilization of arbuscular mycorrhizal fungi
A technology of arbuscular mycorrhizal fungi and Solanum nigrum, applied in the field of soil pollution remediation, can solve the problems of no obvious increase in Zn absorption and complicated influence of strengthening effect, achieve good treatment effect, low operating cost, and no secondary pollution effect
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Embodiment 1
[0041] Example 1 Preparation of arbuscular mycorrhizal fungus inoculum
[0042] Arbuscular mycorrhizal fungi Glomus versiforme Provided by the Institute of Plant Nutrition and Resources, Beijing Academy of Agriculture and Forestry Sciences. Using corn ( Zea mays ) as a host plant for potted propagation, the mixture containing host plant root segments, fungal hyphae, mycorrhizal fungal spores and potting soil is used as an inoculant.
[0043] Concrete preparation process is as follows:
[0044] S1. Mix the arbuscular mycorrhizal fungus inoculant with the sterilized sand evenly according to the inoculation amount of 3% according to the mass ratio, and then fill the mixed matrix into 2 / 3 of the aseptic basin;
[0045] The sand:soil ratio of the sandy soil is 1:1; the arbuscular mycorrhizal fungus inoculant is provided by the Institute of Plant Nutrition and Resources, Beijing Academy of Agriculture and Forestry Sciences.
[0046] S2. Sow 10-15 corn seeds / pot, cover with a...
Embodiment 2
[0052] Example 2 Study on Enhanced Solanum Solanum Uptake of Cadmium in Soil by Arbuscular Mycorrhizal Fungi
[0053] 1. Experimental materials
[0054] (1) Test plant Solanum nigrum
[0055] The test plant Solanum nigrum ( Solanum nigrum ), sterilize the surface of the seeds with 10% NaClO for 10 minutes before sowing, wash them with deionized water, soak the washed Solanum nigrum seeds in ultra-pure water for 4-6 hours, wait until the seeds are fully swollen, and put them in a pad with filter paper Put them in a sterile petri dish at room temperature of 28°C. After the seeds germinate, select seedlings with consistent growth for later use.
[0056] (2) The soil to be tested was collected from the Biological Garden of South China Normal University. The soil was air-dried naturally, ground, passed through a 1mm sieve, and sterilized by continuous high-temperature and high-pressure steam for 2 hours (121°C), and different concentrations of CdCl were added 2 , so that the C...
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