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Semen ziziphi spinosae alkaloid monomer composition zizyphusine, and preparation method and application thereof

A technology of jujube seed and alkaloids, applied in the directions of pharmaceutical formulation, drug combination, drug delivery, etc., can solve the problems of large dosage of traditional Chinese medicine and many side effects of antidepressants, achieve no toxic side effects, prevent and treat insomnia, Preparation method for stable and reproducible results

Active Publication Date: 2014-08-13
汉方萃取生物科技(海南)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention provides a sour jujube seed alkaloid monomer component acid plum base and its preparation method and application, which solves the problems of many side effects of antidepressants and large dosage of traditional Chinese medicine in the prior art

Method used

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  • Semen ziziphi spinosae alkaloid monomer composition zizyphusine, and preparation method and application thereof
  • Semen ziziphi spinosae alkaloid monomer composition zizyphusine, and preparation method and application thereof
  • Semen ziziphi spinosae alkaloid monomer composition zizyphusine, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for extracting and separating plum alkali from Suanzaoren, the method comprising the following steps:

[0035] Impregnate 120g of jujube seed with 7 times and 4 times (w / v) 0.5% hydrochloric acid for 2 times, each time for 24 hours, combine the filtrate, centrifuge to get the supernatant, and absorb it with X-5 macroporous adsorption resin column, distilled water After elution, elute with 70% ethanol solution, and recover ethanol by distillation under reduced pressure to obtain the total alkaloids of Suanzaoren (3.07g).

[0036] The alkaloids of Jujube Seed are dissolved in distilled water (w / v=5:1), and the pH is adjusted to 12 with ammonia water. Extract with n-butanol solution, distill the organic layer under reduced pressure, and recover the solvent to obtain the n-butanol layer extract. The n-butanol layer extract was further separated by neutral alumina column chromatography, and the elution gradient was: 100% methanol, methanol-water (1:1), and 100% wat...

Embodiment 2

[0039] Serum Medicinal Chemistry Method Plumbine is the active ingredient of jujube seed alkaloids entering the blood.

[0040] 1. Experimental animals

[0041] Healthy male ICR mice (SPF grade, body weight 20±2g); purchased from the Experimental Animal Center of the Institute of Radiological Medicine, Chinese Academy of Medical Sciences, certificate number: SCXK (Beijing) 2009-0004; raised in the laboratory for 3 days before the experiment, and adapted to environment. The room temperature is 22±2°C, and the relative humidity is 65% to 70%. Sunlight for 12 hours, free diet and water intake.

[0042] 2. Methods and results

[0043] 2.1 Preparation and administration of medicinal liquid

[0044]Precisely weigh the extract of the alkaloid part of Suanzaoren, dissolve it in distilled water, and make a 20mg / mL liquid medicine. 10 mice were randomly numbered, among which Nos. 1 to 5 were the model group, and Nos. 6 to 10 were the administration group; the model group was given ...

Embodiment 3

[0050] Pharmacodynamic test of alkaloid monomer Libine in jujube seed

[0051] 1. Materials

[0052] 1.1 Experimental animals: Experimental animals: 40 male ICR mice (SPF grade, body weight 20±2g) were purchased from Beijing Huafukang Biotechnology Co., Ltd., Chinese Academy of Medical Sciences, Chinese Union Medical University Institute of Experimental Animals, certificate number: SCXK (Beijing) 2009-0008. Raised in the laboratory for 3 days before the experiment to adapt to the environment. The room temperature is 22±2°C, and the relative humidity is 65% to 70%. Light for 12 hours, free to eat and drink.

[0053] 1.2 Drugs and reagents: Suanzaoren (Anguo Qirui Traditional Chinese Medicine Co., Ltd., batch number: 100607, identified by the Department of Medicine, Tianjin Medical University); Jieyu Anshen Granules (Jilin Minmintang Pharmaceutical Co., Ltd., batch number: 20100103075), hydrochloric acid Venlafaxine extended-release capsules (Effexor, Wyeth, Ireland, batch n...

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Abstract

The invention discloses a semen ziziphi spinosae alkaloid monomer composition zizyphusine, and a preparation method and application thereof. Zizyphusine is prepared by performing adsorption on semen ziziphi spinosae medicinal material by macroporous adsorption resin column to obtain semen ziziphi spinosae total alkaloids, performing further separation on extractive by alumina column chromatography, and finally separating eluates by HPLC. The preparation method is stable, repeatable, and high in yield, and the obtained high-purity monomer composition can be used as a contrast sample. Pharmacodynamic experiments show that the semen ziziphi spinosae alkaloid monomer zizyphusine is the active composition of semen ziziphi spinosae for giving play to resolve stagnation for tranquilization, has the effect of preventing and treating insomnia and depression, is applicable to research and development of antidepressants, is free of toxic and side effects, and has no disadvantages such as exciting uneasiness, anuria, constipation, vertigo, tremble and the like possessed by conventional antidepressants.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract and a preparation method thereof, in particular to the jujube seed alkaloid monomer component and its preparation method and application. Background technique [0002] With the intensification of social competition and the acceleration of the pace of life, the incidence of emotional diseases such as depression and insomnia is rising sharply. Patients are unable to study and work normally, and their quality of life has dropped sharply. For the treatment of depression, many antidepressants have been available since the 1950s. According to their pharmacological effects, they can be divided into: serotonin reuptake inhibitors, monoamine oxidase inhibitors, phenylpiperazine derivatives, serotonin-norepinephrine reuptake inhibitors, aminoketone antidepressants, tricyclic antidepressants, etc. However, western medicine has the disadvantages of limited curative effect, easy drug resistance and o...

Claims

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Application Information

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IPC IPC(8): C07D221/18A61K9/20A61K9/72A61P25/20A61P25/24
CPCC07D221/18
Inventor 乔卫段宏泉周晶秦雪曹相兰符敬伟
Owner 汉方萃取生物科技(海南)股份有限公司
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