Application of adopting Mojave Bacillus KJS-3 as feed additive

A technology of Bacillus virulina and Bacillus, applied in application, animal feed, animal feed, etc., can solve the problems of complex fermentation production process and difficult separation of active substances, and achieve the effects of short fermentation cycle, low production cost and high concentration

Inactive Publication Date: 2014-08-06
武汉虹睿生物科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are not many studies on Mojavebacillus at present, and there are still many problems such as complex fermentation production process and difficult separation of active substances, but with the rapid development of biotechnology, Mojavebacillus has great potential and development value will be confirmed one by one

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  • Application of adopting Mojave Bacillus KJS-3 as feed additive
  • Application of adopting Mojave Bacillus KJS-3 as feed additive
  • Application of adopting Mojave Bacillus KJS-3 as feed additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1. Attachment rate experiment of Bacillus mojave KJS-3

[0017] experiment method:

[0018] 1. First set the concentration to 1X10 5 Cells / 4ml Caco-2 cells are cultured in tissue culture dishes.

[0019] 2. Prepare the strain and the control strain (L.rhamnosus GG) respectively into 4.1X10 5 cfu / ml, 4.9X10 5 The solution with a concentration of cfu / ml is added to the pre-prepared Caco-2 cell culture medium.

[0020] 3. At 37℃, 5% CO 2 In the incubator, culture the two groups of petri dishes for 2 hours.

[0021] 4. After the culture is completed, treat it with sterile phosphate buffer (pH=7.4) for 3 times, then recover the attached cells with trypsin, wash with PBS, and then cover with TSA medium.

[0022] Experimental results: The control strain (L.rhamnosus GG) is recognized as the strain with the highest attachment rate in the world, and its attachment rate reaches 83.6±6.8%, but the commonly used strain with high attachment rate is Lactobacillus caseiTMC0409, and its a...

Embodiment 2

[0023] Example 2, Antibacterial Experiment of Bacillus Mojave KJS-3

[0024] In order to test the antibacterial characteristics of bacillus mojavensis KJS-3, bacillus mojavensis KJS-3 was tested against two subtypes of super virus resistant vancomycin-resistant enterococcus (VRE) Experiment with 4 subtypes of drug-resistant Staphylococcus aureus (MRSA):

[0025] Test method—take an appropriate amount of strains from a petri dish containing Bacillus mojave KJS-3 and place them in a test tube containing TSB culture medium to make 4 ml of bacterial culture medium. After overnight culture, inoculate it in 100 ml on the next day Continue to culture in TSB culture broth for more than 3 hours. After the culture is completed, put 100 ml of bacterial broth into the fermentor to prepare 4 liters of fermentation broth. After another 2 days, centrifuge, take the supernatant and add 3 moles of hydrochloric acid for pH. After adjusting the pH to 2, put it in a 4°C freezer for storage, and then ...

Embodiment 3

[0030] Example 3 Experiment of primary metabolite of Bacillus mojave KJS-3

[0031] The primary metabolite of bacillus mojavensis KJS-3 produces various enzymes, vitamins and other nutrients. The present invention carries out API50CHB enzyme activity test and vitamin test experiment.

[0032] API50CHB enzyme activity test: This test is a common typical experiment to identify bacterial enzyme activity. It is a simple medium composed of API50CH test strips of 49 fermentable carbohydrates. First inoculate the strain of Bacillus mojave KJS-3 into 100 ml of TSB culture medium and cultivate for more than 8 hours, centrifuge to obtain the supernatant, and inoculate an appropriate amount of the suspension of this strain into each tube of the test strip. , Due to the acid production of fermented carbohydrates and the decrease of the pH value, the indicator changes color. The darker the color, the stronger the enzyme activity, so as to identify the type and strength of enzymes produced by B...

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Abstract

The invention discloses application of adopting Mojave Bacillus KJS-3 as a feed additive. The preservation number of the Mojave Bacillus KJS-3 is KCCM10961P; the endospore conversion rate is higher than 99%; industrialized production can be realized; the strain (namely the Mojave Bacillus KJS-3) provided by the invention has broad-spectrum anti-microbial characteristic on various pathogenic fungi and pathogenic bacteria particularly harmful microorganism such as vancomycin-resistant enterococcus and resistant staphylococcus aureus; the strain can generate various digestive enzyme, vitamin, lactic acid, and other substances, is probiotics which are safe and nontoxic for animals, can be used as the feed additive, and is proved to be capable of promoting animal growth.

Description

Technical field [0001] The invention relates to the application of Bacillus mojave KJS-3 as a feed additive and belongs to the field of microorganisms. Background technique [0002] Bacillus mojave is a new type of Bacillus isolated from the Mojave Desert in 1994. It is closely related to Bacillus amyloliquefaciens, Bacillus atrophicus and Bacillus subtilis in the taxonomy of species. relationship. Initially in 1996, Bacillus mojave was confirmed to be a plant endophyte, which can reduce the accumulation of mycotoxins produced by endophytic fungi in plants. After further research, it was confirmed that all strains of the genus Mojave Bacillus have endophytic antagonistic fungus effects. And it was confirmed in 2002 that this kind of bacteria can survive in the intercellular spaces of plants to promote plant growth. In 2007, experiments confirmed that it has a strong control effect on wheat head blight caused by Fusarium graminearum. The banana "Panama disease" in Hainan Provin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K10/18
Inventor 姜在璿欧黎虹刘杰
Owner 武汉虹睿生物科技开发有限公司
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