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Tyrosinase inhibitormicro-emulsion and preparation method and application thereof

A tyrosinase and inhibitor technology, which is applied in the field of tyrosinase inhibitor microemulsion and its preparation and application, can solve the problems of natural tyrosinase inhibitor insolubility, etc. Active, inexpensive effect

Inactive Publication Date: 2014-08-06
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The first technical problem to be solved in the present invention is to provide a kind of tyrosinase inhibitor microemulsion, especially for the high activity of tyrosinase inhibitor steppogenin (grass eugenin) or artocarpanone (cinnamon dihydroflavin) Active, non-toxic, nano-sized natural tyrosinase inhibitor microemulsion to solve the problem of insolubility and low solubility of natural tyrosinase inhibitors in water

Method used

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  • Tyrosinase inhibitormicro-emulsion and preparation method and application thereof
  • Tyrosinase inhibitormicro-emulsion and preparation method and application thereof
  • Tyrosinase inhibitormicro-emulsion and preparation method and application thereof

Examples

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Embodiment 1

[0029] The preparation method of embodiment 1 Steppogenin sample

[0030] Steppogenin is extracted from jackfruit. 2kg of jackfruit wood (the wood is collected from Wenchang County, Hainan Province) is ultrasonically extracted twice with 6 times the amount of 95% ethanol for 1 hour each time, concentrated to dryness under reduced pressure, and weighed. Then dissolve with 95% ethanol, filter with qualitative filter paper, add to Amberlite XAD16, dry naturally or evaporate to dryness under reduced pressure, put on Amberlite XAD16 chromatography column, use 20%, 30%, 40%, 50% and 95% ethanol respectively Eluted with 6 column volumes, collected 40% ethanol eluted part, concentrated to obtain a dry sample, dissolved in methanol and separated by glucose gel column chromatography, eluted with methanol-water (v / v, 1:1), namely The compound steppogenin was obtained.

[0031] The NMR spectral data of Steppogenin are as follows:

[0032] 1 H NMR (Acetone-d 6 ,400MHz)δ:12.21(1H,s,OH-5...

Embodiment 2

[0033] The preparation method one of embodiment 2 Steppogenin microemulsion

[0034]Steppogenin is a natural flavonoid tyrosinase inhibitor that exists in Moraceae plants. It has a strong tyrosinase inhibitory activity, but it is difficult to dissolve in water. The experimental measurement shows that its solubility in water is 8.19% μg / mL.

[0035] Steppogenin microemulsion preparation process: Tween 80 is selected as the surfactant, polyethylene glycol is selected as the co-surfactant, and ethyl butyrate is used as the oil phase. At 20°C, select the mass ratio of the oil phase, surfactant and co-surfactant to be 2:5.3:2.7, mix Tween 80 and polyethylene glycol at a mass ratio of 5.3:2.7 for 3 hours, and then mix the active agent Mix with ethyl butyrate at a mass ratio of 8:2, stir evenly, take 2g of the mixture, add 10mg of steppogenin, and ultrasonically dissolve it completely at 20°C for 30min. Finally, 8 g of water was added by dripping method to make the water content re...

Embodiment 3

[0040] The preparation method two of embodiment 3 Steppogenin microemulsion

[0041] With reference to the preparation method of Example 2, with Tween 80 as surfactant, with ethanol as co-surfactant, with ethyl butyrate as oil phase, the mixture of oil phase, surfactant and co-surfactant three The ratio is 20%:53%:27%, the concentration of steppogenin in the obtained microemulsion system is 0.93mg / mL, the water content is 80%, the system is clear, transparent and stable, and the solubility of steppogenin is increased by 114 times.

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Abstract

The invention discloses a tyrosinase inhibitormicro-emulsion and a preparation method and an application of the tyrosinase inhibitor micro-emulsion, and belongs to the technical fields of foods, medicines and cosmetics. The micro-emulsion comprises a ground substance, tyrosinase inhibitors including steppogenin and artocarpanone, and water, wherein the ground substance comprises 45%-60% of surfactant, 20%-30% of cosurfactant and 10%-30% of oil phase. The micro-emulsionis prepared from various materials according to certain ratios. The defect of extremely low solubility of the tyrosinase inhibitors including steppogenin and artocarpanone in the water is overcome, the method which is simple in operation and can spontaneously form the micro-emulsion is provided, and the water or an aqueous solution is used for dilution so as to prepare the micro-emulsion with nanoscale grain size. The prepared micro-emulsion can be effectively used for preventing enzymatic browning of fruits and vegetables, preparing skin whitening cosmetics, preventing and treating chromatosis diseases and melanoma of a human body that are caused by excessive melanin, and preparing other medicines requiring inhibition of the tyrosinaseactivity.

Description

technical field [0001] The invention relates to a tyrosinase inhibitor microemulsion and its preparation method and application, belonging to the technical fields of food, medicine and cosmetics. Background technique [0002] Tyrosinase (EC1.14.18.1), a kind of polyphenol oxidase, widely exists in organisms. It catalyzes the hydroxylation of monophenols to o-diphenols, then further oxidizes the o-diphenols to o-diquinones, and then undergoes a series of complex non-enzymatic reactions to finally generate melanin. In this process, tyrosinase plays a key role. The melanin produced in the human body can protect the human skin from the sun's ultraviolet rays. At the same time, the content and type of different melanin in the skin determine the skin color and hair color of human beings, but excessive melanin secretion will cause serious cosmetic problems, such as spots, Dark spots, age spots, etc. During food processing, tyrosinase catalyzes browning of fresh fruits, vegetable...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/154A61K8/97A61K8/49A61Q19/02A61K31/352A61K9/107A61P17/00
Inventor 郑宗平陈洁袁佳丽
Owner JIANGNAN UNIV
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