Artificial culturing method of lepista sordida mycelium and culturing medium thereof
A technology of A. variegata and culture medium, which is applied in the field of pharmacognosy. It can solve the problems that restrict the artificial domestication and cultivation of variegated variegated mushroom, reduce wild resources, and have many branched hyphae, and achieve the effects of shortening the cultivation period, low yield, and fresh taste.
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Embodiment 1
[0025] (1) Pretreatment of pine needles:
[0026] Wash the collected 30g pine needles, put them into 1000ml water, boil for 20-30 minutes, filter to get the juice;
[0027] (2) Culture medium preparation:
[0028] Dilute the pine juice to 1000ml, then add 5g of peptone, 6g of yeast extract powder, 2.5g of glucose, and 15g of agar in turn, boil and mix well, and then pack;
[0029] (3) Subpackage and sterilize the medium:
[0030] Divide the prepared medium into test tubes, cover with rubber stoppers, and sterilize at 120-130°C for 20-30 minutes;
[0031] (4) cooling, inoculation:
[0032] After sterilization, take out the culture medium, cool it down to 20°C-30°C naturally, make a slant medium, and insert the purified mushroom strains under aseptic conditions;
[0033] (5) Cultivate:
[0034] Put the inoculated culture medium in a constant temperature incubator, and cultivate it at 20-30°C for 5-10 days until the mycelium is full of the test tube;
[0035] (6) Preserv...
Embodiment 2
[0037] (1) Pretreatment of pine needles:
[0038] Wash the collected 36g pine needles, put them into 1000ml water, boil for 20-30 minutes, filter to get the juice;
[0039] (2) Culture medium preparation:
[0040] Dilute the pine juice to 1000ml, then add 8g peptone, 6g yeast extract powder, 4g glucose, 18g agar in turn, boil and mix well, and then pack;
[0041] (3) Subpackage and sterilize the medium:
[0042] Divide the prepared medium into test tubes, cover with rubber stoppers, and sterilize at 120-130°C for 20-30 minutes;
[0043] (4) cooling, inoculation:
[0044] After sterilization, take out the culture medium, cool it down to 20°C-30°C naturally, make a slant medium, and insert the purified mushroom strains under aseptic conditions;
[0045] (5) Cultivate:
[0046] Put the inoculated culture medium in a constant temperature incubator, and cultivate it at 20-30°C for 5-10 days until the mycelium is full of the test tube;
[0047] (6) Preservation of strains: ...
Embodiment 3
[0049] (1) Pretreatment of pine needles:
[0050] Wash the collected 50g pine needles, put them into 1000ml water, boil for 20-30 minutes, filter to get the juice;
[0051] (2) Culture medium preparation:
[0052] Dilute the pine juice to 1000ml, then add 12g peptone, 12g yeast extract powder, 10g glucose, 20g agar in turn, boil and mix well, and then pack;
[0053] (3) Subpackage and sterilize the medium:
[0054] Divide the prepared medium into test tubes, cover with rubber stoppers, and sterilize at 120-130°C for 20-30 minutes;
[0055] (4) cooling, inoculation:
[0056] After sterilization, take out the culture medium, cool it down to 20°C-30°C naturally, make a slant medium, and insert the purified mushroom strains under aseptic conditions;
[0057] (5) Cultivate:
[0058] Put the inoculated culture medium in a constant temperature incubator, and cultivate it at 20-30°C for 5-10 days until the mycelium is full of the test tube;
[0059] (6) Preservation of strain...
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