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Acremonium cucurbitacearum and application thereof in generating anthraquinone substances

A technology of Acremonium acremonium and anthraquinone, which is applied in the field of liquid fermentation medium formula of Acremonium acremonium and anthraquinone compounds produced by it

Inactive Publication Date: 2014-07-02
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After that, there is still no research on bamboo yellow anthraquinone abroad, and there are few domestic researches, mainly on its separation, purification and structure.

Method used

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  • Acremonium cucurbitacearum and application thereof in generating anthraquinone substances
  • Acremonium cucurbitacearum and application thereof in generating anthraquinone substances
  • Acremonium cucurbitacearum and application thereof in generating anthraquinone substances

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1 The separation of anthraquinone-producing substance strains

[0012] (1) Tissue separation method

[0013] The strains were separated by the tissue separation method, that is, to select a relatively fresh Bamboo chinensis, first sterilize its surface with 75% ethanol, and then use a sterilized blade to cut the fruiting body into about 0.4mm×0.4mm× Small pieces of 0.4mm~0.6mm×0.6mm×0.6mm are sterilized in 75% alcohol for 2 minutes, then transferred to 1% mercuric chloride solution for 2 minutes, rinsed with sterile water for 4 to 5 times, and placed Put 3 to 4 tissue pieces in each plate on the plate with strain isolation medium, cultivate in a constant temperature incubator at 27°C, and observe the growth of the colony.

[0014] The observed results are: after 1 day of culture, no hyphae can be observed on the surface of the medium and tissue blocks; after 2 days of culture, white hyphae can be seen on the medium and on the surface of most tissue blocks; It ...

Embodiment 2

[0017] The acquisition of embodiment 2AJ014 bacterial strain

[0018] (1) Intuitive screening

[0019]Naturally occurring quinones are yellow, orange, brownish red, or even purple in color due to the introduction of chromogenic groups such as phenolic hydroxyl groups on the core. In the initial stage of strain screening, the most intuitive way to judge is to observe whether the strain produces pigment in the medium. For the strains that have been isolated in Example 1, the strains that have the ability to produce pigment are screened in a targeted manner, inserted into a new potato dextrose agar medium plate, and cultured at 27°C. The pigment-producing strains are repeatedly purified to remove miscellaneous bacteria, and then inserted into Potato dextrose agar slant medium, cultured at 27°C, and preserved at 4°C after colonies covered the slant. One of the strains grows vigorously on potato dextrose agar. With the prolongation of culture time, the color of mycelium gradually...

Embodiment 3

[0031] Observation and identification of embodiment 3AJ014 bacterial strain

[0032] The AJ014 strain was cultured on a plate, and its growth status and colony morphological characteristics in different periods were observed and recorded. Generally, after 10-15 days of culture, use a microscope to observe whether the strain has formed a sporulation structure; if it does not have a sporulation structure, conduct a sporulation induction experiment on the strain, including prolonging the culture time, improving the medium formula, ultraviolet induction, and environment. Temperature control, environmental light control and other methods; study the characteristics of its sporulation structure and spore structure, according to these characteristics, according to the principle of morphological taxonomy of fungi, identify the classification status of the AJ014 strain as Fungi Ascomycetes Phylum Ascomycota Asexual Hyphomycetes Hyphomycetes Moniliales Moniliaceae Acremonium Acremonium c...

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Abstract

The invention belongs to the field of utilization of microbial resources and relates to acremonium cucurbitacearum and an application thereof in generating anthraquinone substances. The number of an acremonium cucurbitacearum strain is AJ014, and the preserving number is CGMCC (China General Microbiological Culture Collection Center) NO. 8729. The strain can produce the anthraquinone substances through liquid fermentation. The invention further discloses a fermentation formula and fermentation conditions. The acremonium cucurbitacearum strain and the fermentation formula and the fermentation conditions for generating the anthraquinone substances can be provided for broadened industrial production.

Description

technical field [0001] The invention relates to the field of microbial resource utilization, and relates to the cultivation and fermentation of microbial strains producing anthraquinone natural active products in the microbial pharmaceutical industry, specifically a liquid fermentation medium formula for acremonium cucurbita and the anthraquinone compounds it produces . Background technique [0002] Quinone compounds are a general term for compounds that exist in nature and have an unsaturated cyclic diketone structure (quinone structure) or are easily transformed into such structures. According to the number of aromatic rings and combination conditions, they are divided into four categories: benzoquinone, phenanthrenequinone, naphthoquinone and anthraquinone. Anthraquinone is the most important class of quinone compounds, which has various physiological activities such as antidiarrheal, antibacterial, diuretic, hemostatic, anticancer and antiviral. Nearly 200 kinds of ant...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P1/02C12R1/645
Inventor 陈双林黄珍闫淑珍
Owner NANJING NORMAL UNIVERSITY
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