A kind of plant tissue culture explant anti-browning disinfectant and using method

A technology of plant tissue culture and explants, which is applied in the field of plant tissue culture, can solve the problems of large damage, environmental pollution of plant cultures, and human poisoning, and achieve the effects of improving survival rate, environmental friendliness, and low cost

Inactive Publication Date: 2016-03-09
SUBTROPICAL CROPS INST OF FUJIAN PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention solves the technical problems that the explant disinfectant for plant tissue culture is poisonous to the human body, seriously pollutes the environment and causes great damage to the plant culture

Method used

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  • A kind of plant tissue culture explant anti-browning disinfectant and using method
  • A kind of plant tissue culture explant anti-browning disinfectant and using method

Examples

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Embodiment 1

[0015] Phalaenopsis (Phalaenopsis pp.) leaves were cut into pieces and rinsed with running water for more than 30 minutes, soaked in 75% ethanol for 30 seconds, washed twice with sterile water, and soaked and disinfected with the following methods respectively (1) soaked in 2% sodium hypochlorite for 30 minutes, rinsed with sterile water 3 times. (2) Soak in 0.1% mercuric chloride for 8 minutes, rinse with sterile water for 5 times. (3) Soak in 0.5% tetrakis hydroxymethyl phosphorus sulfate solution for 60 minutes, rinse with sterile water for 3 times, then soak in 0.5% 2-benzyl-4-chlorophenol solution for 30 minutes without rinsing with sterile water, and blot dry with filter paper Surface solution, direct inoculation. Medium composition 1 / 2MS+6-BA5mg / L+NAA0.2mg / L+coconut juice 20% (V / V). Each bottle was inoculated with 6 explants, 5 bottles each time, and repeated 3 times. After inoculation, culture at 25°C under 1500lux light, and light for 12h / d.

[0016] After Phalaen...

Embodiment 2

[0021] Milan (Aglaiaodorata) segmented stem, rinsed with running water for 2 hours, soaked in 75% ethanol for 30 seconds, washed twice with sterile water, soaked and disinfected with the following methods respectively (1) soaked in 2% sodium hypochlorite for 30 minutes, rinsed with sterile water for 3 minutes Second-rate. (2) Soak in 0.1% mercuric chloride for 12 minutes, rinse with sterile water 5 times. (3) Soak in 1% tetrakis hydroxymethyl phosphorus sulfate solution for 60 minutes, rinse 3 times with sterile water, soak in 0.5% 2-benzyl-4-chlorophenol solution for 60 minutes, rinse without sterile water, and dry the surface with filter paper solution, direct inoculation. (4) Soak in 0.5% tetrakis hydroxymethyl phosphorus sulfate solution for 60 minutes, rinse 3 times with sterile water, soak in 0.5% 2-benzyl-4-chlorophenol solution for 40 minutes, rinse without sterile water, and dry the surface with filter paper solution, direct inoculation.

[0022] The composition of...

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Abstract

The invention discloses an anti-browning sanitizer for a plant tissue culture explant, and belongs to plant tissue culture technologies in agriculture and forestry fields. A method in which the surface of the explant is sterilized with a reducible sterilant which replaces conventional mercuric chloride or sodium hypochlorite comprises steps of washing the tissue culture explant with flowing water, then sterilizing instantly with an ethanol solution, and soaking the explant respectively through tetrakis hydroxymethyl phosphonium sulfate and a 2-benzyl-4-chlorophenol solution in sequence. Compared with a conventional explant sanitizer, the reducible sterilant is efficient, has the broad spectrum, is mild to plant cells, inhibits browning while sterilizing the explant, improves the pretreatment efficiency of the explant, lowers the death rate of the tissue culture explant, is simple and convenient to operate, is safe to people, is convenient in popularization and application and has the environment-friendly effect.

Description

technical field [0001] The invention belongs to plant tissue culture technology in the field of agriculture and forestry, in particular to methods for disinfection and anti-browning of tissue culture explants. technical background [0002] Plant tissue culture technology has broad application prospects in seedling production, virus removal, and germplasm improvement. At present, there are still many problems in the technical aspects of plant tissue culture, and the prevention and control of explant contamination is the main difficulty in the success of tissue culture. The conventional explant disinfection method is to soak in 70% alcohol for 10-30s, rinse with sterile water 2-3 times, soak in 0.1% mercury chloride for 8-12min or use 2% sodium hypochlorite (saturated calcium hypochlorite solution) Soak for 15-20min. Although mercuric chloride has a good disinfection effect, it volatilizes in a small amount at room temperature and can penetrate the skin. It is extremely toxi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N3/00A01H4/00
Inventor 刘福平
Owner SUBTROPICAL CROPS INST OF FUJIAN PROVINCE
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