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A kind of Agrobacterium with autogenous nitrogen fixation, phosphorus and potassium decomposing ability and its application

A technology of Agrobacterium and nitrogen fixation, applied in the field of agricultural microorganisms, can solve problems such as short validity period, low nitrogen fixation amount, unstable application effect, etc.

Inactive Publication Date: 2016-08-17
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the nitrogen-fixing bacteria strains used in production in my country have low nitrogen-fixing capacity and unstable application effects, and most of the wild nitrogen-fixing bacteria isolated from nature have slow growth, low survival rate, short validity period, nitrogen fixation and growth promotion ability. Low and harsh requirements on the soil environment, etc., cannot meet the needs as a growth-promoting microbial fertilizer

Method used

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  • A kind of Agrobacterium with autogenous nitrogen fixation, phosphorus and potassium decomposing ability and its application
  • A kind of Agrobacterium with autogenous nitrogen fixation, phosphorus and potassium decomposing ability and its application
  • A kind of Agrobacterium with autogenous nitrogen fixation, phosphorus and potassium decomposing ability and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The nitrogen-fixing bacterial strain of the present invention is obtained by the method of ultraviolet-plasma compound mutagenesis, and the specific steps are as follows:

[0049] 1) Use the Agrobacterium SGD06 screened from the plant rhizosphere in the laboratory as the starting strain;

[0050] 2) Mutation breeding

[0051] (1) Prepare the single cell suspension of the starting strain SGD06

[0052] Inoculate the starting strain SGD06 in liquid medium A, culture at 28-32°C, 120-150rpm for 15-20 hours, centrifuge, wash with sterile saline, place in a Erlenmeyer flask with glass beads, shake to make it Disperse into a single-cell bacterial suspension;

[0053] The composition of the medium A is: yeast powder 5-10g, NaCl 2-5g, peptone 5-10g, FeSO 4 ·7H 2 O 0.005-0.01g, Na 2 MoO 4 2H 2 O 0.0025-0.005g, distilled water 1000ml, pH 7.0~7.2.

[0054] (2) Ultraviolet mutagenesis

[0055] Adjust the concentration of the bacterial suspension obtained in step (1) to 10 ...

Embodiment 2

[0077] Example 2 Determination of the ability of SGD06-8-33 to produce indole acetic acid (IAA)

[0078] Add a concentration of 200 μg·ml to a 250 ml Erlenmeyer flask containing 100 ml of liquid medium A -1 Aseptic L-tryptophan, inoculate SGD06-8-33 in 50ml liquid medium A, culture at 28°C, 150rpm for 20 hours, take 100 μl (dilute the bacterial solution with medium A to make it OD 600 0.7) The bacterial solution was inoculated in the above-mentioned liquid medium A containing L-tryptophan, cultured at 28°C, 150 rpm for 72 hours, centrifuged at 4000 g for 10 min, and the concentration of IAA in the supernatant was measured by spectrophotometry. The experimental results showed that: SGD06-8-33 produced IAA concentration of 43.72μg / ml.

Embodiment 3

[0079] Example 3 Determination of the ability of SGD06-8-33 to produce siderophore

[0080] Dispense the liquid medium A into 250ml Erlenmeyer flasks according to 50ml per bottle, and sterilize it for later use. Inoculate SGD06-8-33 in 50ml of liquid medium A, culture at 28°C and 150rpm for 20 hours, take 100 μl (dilute the bacterial solution with medium A to make it OD 600 0.7) The bacteria solution was inoculated in the above-mentioned liquid medium A, and cultured at 28°C and 150 rpm for 48 hours. The bacteria solution cultured on the above-mentioned shaker was taken and centrifuged at 3500 rpm for 15 minutes. The content of siderophore in serum, A / A r=0.406, indicated that SGD06-8-33 had a strong ability to produce siderophore.

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Abstract

The present invention relates to a strain of Agrobacterium with the ability to self-generate nitrogen fixation, phosphorus and potassium solubilization, which is classified as Agrobacterium r sp. Microbiology Center, the deposit number is CGMCC No.5457. The strain has high nitrogenase activity, high ability to decompose phosphorus and potassium, can produce a certain amount of indole acetic acid and siderophore, and has no ACC deaminase activity. Inoculating crops such as corn, rapeseed and mustard with the liquid or solid bacterial agent containing the strain can obviously promote their growth and increase the yield of the crops. The method has simple process, abundant sources of raw materials, and is suitable for industrial production.

Description

technical field [0001] The invention relates to an Agrobacterium strain capable of autogenous nitrogen fixation, phosphorus and potassium dissolution, and application thereof. The strain is obtained by ultraviolet-plasma compound mutagenesis, can promote the growth of crops, and belongs to the technical field of agricultural microorganisms. technical background [0002] Nitrogen is one of the macroelements necessary for crop growth and development, and contributes 40%-50% to the final yield of crops. Crops take away a large amount of nitrogen from the soil every year. In order to supplement the loss of soil nitrogen, industrial chemical nitrogen fertilizers are still an important source of human nitrogen supply to the soil. Although chemical fertilizers have played a huge role in promoting crop growth and increasing food production, long-term excessive use of chemical fertilizers will cause a series of problems, such as acid deposition, soil compaction, water eutrophication,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N15/01C05G3/00C12R1/01
Inventor 朱晓丽朱燕云董立平
Owner NORTHWEST UNIV
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