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Application of procyanidin B2 in medicaments

A proanthocyanidin and drug technology, applied in the field of proanthocyanidin B2 in medicine, to achieve the effects of avoiding damage, enhancing curative effect, and inhibiting and protecting bone marrow DNA

Inactive Publication Date: 2014-05-14
CHENGDU KEER PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] At present, there are no relevant reports on the application of proanthocyanidin B2 in bone marrow protection and treatment of leukopenia

Method used

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  • Application of procyanidin B2 in medicaments
  • Application of procyanidin B2 in medicaments
  • Application of procyanidin B2 in medicaments

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0074] Preparation of reference substance solution: Accurately weigh 50 mg of gallic acid reference substance, put it in a 100ml brown measuring bottle, add water to dissolve and dilute to the mark, accurately measure 5ml, put it in a 50ml brown measuring bottle, dilute with water to the mark, shake well, that is Obtained (every 1ml contains gallic acid 0.05mg).

[0075] Preparation of the standard curve: Accurately measure 0.5ml, 1.0ml, 2.0ml, 3.0ml, 4.0ml, 5.0ml of the reference solution, put them in 25ml brown measuring bottles, add 1ml of phosphomolybdotungstic acid test solution, and then add water respectively 11. 50ml, 11ml, 10ml, 9ml, 8ml, 7ml, dilute to the mark with 29% sodium carbonate solution, shake well, let stand for 30 minutes and use the corresponding reagent as a blank, according to ultraviolet-visible light photometry (Appendix V A), at 760nm Measure the absorbance at the wavelength, draw the standard curve with the absorbance as the ordinate and the concent...

Embodiment 1

[0124] Embodiment 1: the preparation of Burnet tannin extract of the present invention

[0125] Grind Burnet medicinal material into coarse powder, flash extract 2 times with 70% acetone, 2 minutes each time, 10 times the amount of solvent for the first time, 8 times the amount for the second time, combine the filtrate, concentrate under reduced pressure, diethyl ether and concentrated solution 1 : 1 volume ratio extraction and degreasing until the ether layer is colorless, then extract and enrich the total phenols with ethyl acetate and mother liquor 1:1 volume ratio 6 times, combine ethyl acetate, concentrate under reduced pressure at 45°C to an appropriate amount, and reduce pressure at 45°C Dry and serve.

[0126] The results of content determination are: the extract contains 62% tannin, 1.32% burnet, 2.61% catechin, 20.08% proanthocyanidin B, 6.2% burnet saponin, 3.7% burnet Ⅰ: 3.7%, all by weight percentage content.

Embodiment 2

[0127] Embodiment 2: the preparation of Burnet tannin extract of the present invention

[0128] Grind Burnet medicinal material into coarse powder, reflux and extract with 70% ethanol for 3 times, each time for 2 hours, the amount of solvent is 10 times for the first time, 8 times for the second time, 6 times for the third time, combine the filtrates, and concentrate under reduced pressure , diethyl ether and concentrated solution at a volume ratio of 1:1 to extract and degrease until the ether layer is colorless, then extract and enrich total phenols with ethyl acetate and mother liquor at a volume ratio of 1:1 for 6 times, combine ethyl acetate, and concentrate under reduced pressure at 45°C to Appropriate amount, dried under reduced pressure at 45°C.

[0129] The results of content determination are: the extract contains 65% tannin, 1.41% burnet, 2.65% catechin, 20.09% proanthocyanidin B, 5.4% burnet saponin, 3.1% burnet Ⅰ: 3.1%, all by weight percentage content.

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Abstract

The invention provides an application of procyanidin B2 in preparing medicaments for protecting bone marrow cells. In one embodiment, the procyanidin B2 is a compound with content of 100%. In another embodiment, the procyanidin B2 is a 0.05-1.5 wt% mixture, preferably 0.05-0.4 wt% mixture. According to the application provided by the invention, preferably, the mixture also contains at least one of catechin and sanguiin. The procyanidin B2 can protect bone marrow hematopoietic stem cells, relieve mouse bone marrow DNA (deoxyribonucleic acid) inhibition caused by radiotherapy or chemotherapy, and increase white blood cell count.

Description

[0001] This application is a divisional application with application number 201180038931.5. The filing date of the parent application is September 14, 2011. The invention name of the parent application is a Burnet tannin extract and its preparation method and application. technical field [0002] The invention relates to the use of proanthocyanidin B2 in medicine. Background technique [0003] Myelosuppression is a decrease in the activity of blood cell precursors in the bone marrow. Both red and white blood cells in the blood originate from hematopoietic stem cells in the bone marrow. The blood cells in the blood are short-lived and often need to be replenished constantly. Stem cells, the precursors of blood cells, must divide rapidly for timely replenishment. Chemotherapy, radiation therapy, and many other anti-tumor treatments are aimed at rapidly dividing cells, which often lead to the suppression of normal bone marrow cells, causing bone marrow hematopoietic dysfuncti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/353A61K36/739A61P7/06A61P7/00
CPCA61K31/352A61K36/739A61P19/08A61P35/00A61P7/00A61P7/06A61K31/7034C07D311/62C07H13/04C07H15/256
Inventor 王达宾苏柘僮杨明杨胜邹文铨
Owner CHENGDU KEER PHARMA
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