Method for preparing beta-amylase and soybean oligosaccharides

Abstract

The invention discloses a method for preparing beta-amylase and high-purity soybean oligosaccharides at the same time. The method comprises the following steps: (1) adjusting the pH value of soybean protein wastewater, and adding a filter aid and a composite precipitator; (2) adding desalting water and calcium salt; (3) performing ultrafiltration membrane treatment; (4) performing ultrafiltration membrane treatment to prepare beta-amylase; (5) reducing the electric conductance by an electrodialysis method; (6) fermenting by yeasts and ultra-filtering; (7) performing chromatographic separation by a simulated moving bed to obtain soybean oligosaccharides and fructose. The method has the benefits as follows: 1, the soybean protein wastewater is taken as a raw material, so that the resources are fully utilized and the wastewater treatment cost is saved; 2, obtained beta-amylase is high in activity and good in acid resistance and temperature resistance; 3, the process is simple, the cost is low, and obtained soybean oligosaccharides are high in purity.

Description

technical field [0001] The invention relates to a method for simultaneously preparing beta-amylase and high-purity soybean oligosaccharides, belonging to the comprehensive application field of soybean protein wastewater. Background technique [0002] β-amylase is an exo-amylase. When it acts on starch, it cuts off the non-reducing end of the starch molecule to generate maltose. β-amylase is widely found in plants such as barley, wheat, potatoes and beans, as well as Many microorganisms can produce β-amylase. β-amylase is mainly used in the food industry to produce maltose syrup. Compared with β-amylase derived from barley and potato, soybean β-amylase has the characteristics of strong acid resistance, high temperature resistance and good stability. During the production process of maltose syrup Using its acid resistance and temperature resistance, appropriately lowering the pH during saccharification and increasing the saccharification temperature can effectively prevent ba...

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Problems solved by technology

These soluble substances are the main pollution sources of wastewater from soybean deep-processing enterprises. Due to their high BOD and COD, the treatment is not only costly, but also ineffective, and causes double waste.

[0005] Chinese patent 201310177110.0 mentions a method for producing β-amylase from soybean protein wastewater, but the oligosaccharides in it are not effectively extracted, the comprehensive utilization rate of soybean protein wastewater is low, and the patent does not extract the large molecular weight of soybean protein wastewater The protein is separated from the β-amylase, so the resulting β-amylase has low activity

[0006] Chinese patents CN100439382C and CN101538291B all disclose the purification method of high-purity soybean oligosaccharides, but the former treatment method is intermittent operation, the amount of stationary phase and mobile phase is large, the concentration of the separated product is low, and the separation cost is high, so it is not suitable for industrial production; The former used a simulated moving bed to purify soybean oligosaccharides, but because the raw soybean oligosaccharides contained not only functional components stachyose and raffinose, but also non-functional components sucrose, glucose and fructose, and the types of non-functional components were relatively large. Many, when using a simulated moving bed for chromatographic separation, it is difficult to separate a variety of non-functional components from a variety of functional components at the same time. Therefore, although the purity of soybean oligosaccharides has been greatly improved compared with before, but Still can not meet the consumer demand for the quality of soybean oligosaccharide products

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