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Method for extracting agar, fucoidin and protein from gracilaria as raw material by using enzyme process

A fucoidan and enzymatic extraction technology, which is applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., can solve the problems of environmental pollution, energy consumption, little comprehensive utilization, and underutilization of seaweed raw material resources. Achieve the effects of reducing consumption and sewage discharge, good strength and whiteness, and reducing energy consumption

Active Publication Date: 2014-04-02
青岛福创环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are related research and industrialization implementation cases of extracting single products such as agar and fucoidan from Gracilaria in China, for example, patent 200810029075.7 method for ultrasonic acid extraction of Gracilaria polysaccharide sulfate, patent 200910001265.2 Preparation method of Gracilaria polysaccharide, However, there are few relevant reports on the comprehensive utilization of most of the active ingredients in Gracilaria; in addition, the existing extraction process of agar and fucoidan still follows the traditional process, which not only does not make full use of seaweed raw material resources, but also pollutes the environment. , Energy consumption brings huge pressure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Weigh 50 g of Gracilaria, cut it into pieces, grind it into powder with a universal grinder, add 600 mL of water, soak it at room temperature to make it fully swell, then place it in a water bath at 55°C, adjust the pH value of the soaking solution to 6, and add 50 mg Cellulase, react for 8 h; then at the above temperature, adjust the pH value of the system to 9.5, add 20 mg of complex protease, and react for 4 h. Centrifuge the feed liquid after enzymolysis to obtain enzymolysis solution and enzymolysis residue, wash the Gracilaria residue with water twice, and combine the cleaning solution for soaking in the next batch.

[0029] Gracilaria enzymolysis solution was heated up to 90°C and kept for 10 minutes, then inactivated. The enzymolysis solution was concentrated under reduced pressure to 1 / 3 of its original volume, then adjusted to protein isoelectric point pH=5.0 with dilute hydrochloric acid, and left to stand After 4 h, the precipitate was collected by centrifug...

Embodiment 2

[0032] Weigh 100 g of Gracilaria, cut it into pieces, grind it into powder with a universal grinder, add 1000 mL of water, soak it at room temperature to make it fully swell, then place it in a water bath at 55°C, adjust the pH value of the soaking solution to 6, and add 110 mg Cellulase, react for 8 h; then at the above temperature, adjust the pH value of the system to 8, add 50 mg of complex protease, and react for 2 h. Centrifuge the feed liquid after enzymolysis to obtain enzymolysis solution and enzymolysis residue, wash the Gracilaria residue with water twice, and combine the cleaning solution for soaking in the next batch.

[0033] Gracilaria enzymatic hydrolysis solution was heated to 90°C and kept for 15 minutes, and the enzymatic hydrolysis solution was decompressed and concentrated to 1 / 3 of the original volume, then adjusted to protein isoelectric point pH = 5.0 with dilute hydrochloric acid, and allowed to stand After 4 h, the precipitate was collected by centrifu...

Embodiment 3

[0036] Weigh 100 g of Gracilaria, cut it into pieces, grind it into powder with a universal grinder, add 800 mL of water, soak it at room temperature to make it fully swell, then place it in a 55°C water bath, adjust the pH value of the soaking solution to 6, add 150 mg of cellulase, reacted for 6 h; then at the same temperature as above, adjusted the pH value of the system to 9, added 60 mg of complex protease, reacted for 3 h, after the end of enzymolysis, centrifuged to obtain enzymatic hydrolyzate and enzyme solution residue, the Gracilaria residue was washed twice with water, and the cleaning solution was combined and reserved for soaking in the next batch;

[0037]Gracilaria enzymolysis solution was heated up to 90°C and kept for 20 minutes, and the enzyme activity was inactivated. The enzymolysis solution was concentrated under reduced pressure to 1 / 3 of the original volume, and then adjusted to the protein isoelectric point pH = 5.0 with dilute hydrochloric acid, and le...

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Abstract

The invention discloses a method for extracting agar, fucoidin and protein from gracilaria as a raw material by using an enzyme process. The method comprises the steps of: performing enzymolysis on the gracilaria by using a biological enzymolysis technology, separating protein from enzymatic hydrolysate by using an isoelectric precipitation technology after being concentrated, precipitating the solution after the protein is extracted through alcohol to separate the fucoidin, and performing procedures such as bleaching, glue boiling, freezing, thawing and dewatering on the enzymolysis residue to obtain a high-purity agar product. According to the method, the defect of a process of extracting the fucoidin by using a conventional alkaline process glue extracting and water extraction method is broken, the extraction separation of the agar, the fucoidin and the protein is realized in one step by adopting a biological enzymolysis method, and water saving, energy saving and emission reducing are greatly realized when the comprehensive utilization value of the gracilaria as the raw material is increased. The method accords with relevant national policies.

Description

technical field [0001] The invention belongs to the field of deep processing and comprehensive utilization of marine resources, and in particular relates to a method for enzymatically extracting agar, fucoidan and protein using Gracilaria as raw materials. Background technique [0002] Fucoidin is a unique water-soluble polysaccharide combined with sulfate groups, also known as fucoidan sulfate and fucoidan. It was first extracted from palmate kelp by Kylin in 1913. It is mainly Exist in a variety of brown algae and some marine invertebrates. In recent years, due to the increase of seaweed resources year by year, scholars at home and abroad have conducted a lot of research on the extraction, composition and physiological activities of fucoidan. Studies have shown that fucoidan not only has anti-virus, anti-radiation, hypolipidemic and other physiological activities, but also can be used as an antioxidant nutrient to prevent diseases caused by oxidative stress, and can scave...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/12C08B37/00C07K1/30
Inventor 任宪君孙来娣张秋艳
Owner 青岛福创环境科技有限公司
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