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Method for testing trace of nanogold particle with specific particle diameter

A test method and nano-gold technology, applied in measurement devices, color/spectral property measurement, instruments, etc., can solve the problems of detection recovery rate of only 10%-40%, complex processing, poor specificity, etc., to simplify sample pretreatment Process, high selectivity, low cost effect

Active Publication Date: 2014-03-26
ANHUI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ICP-MS method is expensive, the instrument is expensive, and requires specialized laboratory personnel, complicated sample pretreatment, poor specificity and other disadvantages
And ICP-MS can only be used to detect gold nanoparticles prepared by using 1% HCl (v / v). Only 10%-40%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Synthesis of 1.16nm Gold Particle Whole Antigen

[0030] 1) Synthesis of gold nanoparticles

[0031] Take 100mL of 0.01% chloroauric acid solution and heat it to boiling, then quickly add 5mL of 1% trisodium citrate aqueous solution under stirring, the color of the solution turns black rapidly, and turns purple within 1 minute. until the solution turns transparent wine red, about 7-10 minutes. After the color is stable, continue to reflux and boil for 15 minutes, remove the heat source and continue to stir, and cool to room temperature. The obtained liquid is 16nm gold sol, which is stored at 4°C for later use.

[0032] 2) Preparation of nano-gold immunogen (GNP-BSA)

[0033] Add thioglycolic acid solution to the 16nm gold particle solution under stirring, adjust the solution under weak acid conditions after magnetic stirring for 20 minutes, add EDAC and NHS solutions respectively, stir at room temperature for 3 hours, then adjust the pH to 9-10, and add BSA , after ...

Embodiment 2

[0069] Synthesis of 1.16nm Gold Particle Whole Antigen

[0070] 1) Synthesis of gold nanoparticles

[0071] Take 100mL of 0.01% chloroauric acid solution and heat it to boiling, then quickly add 5mL of 1% trisodium citrate aqueous solution under stirring, the color of the solution turns black rapidly, and turns purple within 1 minute. until the solution turns transparent wine red, about 7-10 minutes. After the color is stable, continue to reflux and boil for 15 minutes, remove the heat source and continue to stir, and cool to room temperature. The obtained liquid is 16nm gold sol, which is stored at 4°C for later use.

[0072] 2) Preparation of nano-gold immunogen (GNP-BSA)

[0073] Add thioglycolic acid solution to the 16nm gold particle solution under stirring, adjust the solution under weak acid conditions after magnetic stirring for 20 minutes, add EDAC and NHS solutions respectively, stir at room temperature for 3 hours, then adjust the pH to 9-10, and add BSA , after ...

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Abstract

The invention relates to a method for testing the trace of a nanogold particle with a specific particle diameter. The method comprises the steps as follows: an antigen of the nanogold particle with the specific particle diameter is prepared; the antigen is injected into an animal; a high specific antibody is prepared; the content of the nanogold particle with the specific particle diameter is measured by adopting an absorbance method; an abzyme is marked to prepare an enzyme label double antibody; by adopting an indirect competitive enzyme-linked immunifaction, a standard sample competes against a sample to be tested to react with the antibody; an enzyme of the enzymbe label double antibody enables a substrate solution to emit light; the value of absorbancy is measured; the density of the sample to be tested is obtained via a standard curve. According to the method, the specificity of the antibody of the nano-gold particle with the specific particle diameter is high, and cross reactions are less, so that the selectivity is high, and further the pretreatment process of the sample is simplified.

Description

technical field [0001] The invention belongs to the technical field of nanometer material detection, and in particular relates to a method for testing a trace amount of nanometer gold particles with a specific particle size. Background technique [0002] Nanomaterials are considered as new materials in the 21st century. Nanostructured materials refer to the particles whose basic component particle size is on the order of nanometers, and are located in the junction area between atomic clusters and macroscopic objects. The particle diameter is generally between 1 and 100 nm, and the particles can be crystal or amorphous. Nanostructured materials have a series of novel physical, mechanical, electronic, magnetic, optical and chemical properties. Nanogold is an association colloid with a diameter of 0.8-250 nm, which has nano-surface effect, quantum effect and macro-quantum tunneling effect, strong oxidation resistance, good biocompatibility, high density and excellent photoelec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N21/31
CPCG01N33/535G01N33/54346G01N33/558
Inventor 张明翠孙锦文
Owner ANHUI NORMAL UNIV
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