Preparation method for drug-carrying multi-layer tissue engineering micro-nano structure bracket

Abstract

The invention discloses a preparation method for a drug-carrying multi-layer tissue engineering micron-nano structure bracket. The preparation method comprises the following steps: firstly, preparing cellosilk by adopting the electrospinning technology, and collecting the cellosilk in a culture dish to obtain a layer of fibrous membrane; preparing microspheres by adopting the micro flow control technology, wherein diameters of the microspheres are controlled within 100-300 microns; mixing the obtained microspheres in oil phase and receiving directly on the fibrous membrane, settling the microspheres on the fibrous membrane, and absorbing away the oil phase after one layer of the microspheres is overspread; spraying a layer of the fibrous membrane on the microspheres directly through the electrospinning technology to obtain a micro-nano structure with a layer of microspheres and two layers of fibrous membranes; repeating n times according to the order to finally prepare a three dimensional frame structure containing micron-sized microspheres and nano-sized fibrous membranes and with n layers of microspheres and n+1 layers of fibrous membranes. The bracket can excellently simulate extracellular matrix environment; additionally, lyophilic drugs and hydrophobic drugs can be respectively added into the microspheres and the fibrous membranes, so that two drugs are released simultaneously to better promote the repairing of tissues.

Description

technical field [0001] The invention relates to a preparation method of a multi-layer tissue engineering micro-nano structure support. Background technique [0002] There are various scaffolds for tissue engineering, such as metal scaffolds, ceramic scaffolds and polymer scaffolds, among which polymer scaffolds are widely used due to their degradability and excellent biocompatibility. Electrospinning technology is one of the commonly used techniques for preparing polymer scaffolds, and can generally prepare nanoscale or submicron non-woven fiber membranes. Microfluidic technology is a technology that can prepare uniform, small-sized, monodisperse microspheres or microcapsules. The prepared polymer tissue engineering scaffolds often hope to have a three-dimensional structure similar to extracellular matrix, so as to mimic the in vivo environment and achieve better therapeutic purposes. Most of the existing electrospun fiber membranes can only provide a two-dimensional struc...

AI Technical Summary

Problems solved by technology

There is no report on the combination of electrospinning technology and microfluidic technology to prepare a three-dimensional scaffold with multi-layer micro-nano structure