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Method for preparing ethyl alcohol by adopting carrier fermenting technique

A technology, ethanol technology, is applied in the field of ethanol preparation by carrier fermentation technology, which can solve the problems of no recovery of fermented strains, inability to perform high-density fermentation, and no immobilization of strains, so as to avoid substrate inhibition and reduce immobilization. Cost, effect of preventing sugar accumulation

Active Publication Date: 2015-07-08
成都市科隆化学品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that the bacteria are not immobilized, high-density fermentation cannot be carried out, and the utilization rate of the equipment is affected; the fermentation bacteria are not recovered, resulting in waste of fermentation bacteria, and it is easy to cause environmental pollution

Method used

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  • Method for preparing ethyl alcohol by adopting carrier fermenting technique
  • Method for preparing ethyl alcohol by adopting carrier fermenting technique
  • Method for preparing ethyl alcohol by adopting carrier fermenting technique

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for producing ethanol through synchronous saccharification and fermentation of mycelium as carrier and fermentation strain:

[0034] 1. Inoculate Saccharomyces cerevisiae strains into a 250ml Erlenmeyer flask containing 100ml of seed medium according to the inoculum amount of 5%, culture it in a shaker at 30°C and 100r / min for 30 hours, and centrifuge the fermentation broth at 5000r / min for 5min. Yeast cells were collected; the seed medium was yeast extract 10 g / L, peptone 20 g / L, glucose 20 g / L, and natural pH.

[0035] 2. Make Aspergillus niger into a spore suspension with sterile water, inoculate it into a 250ml Erlenmeyer flask containing 100ml of enzyme-producing medium according to the inoculum amount of 4%, and cultivate it in a shaker at 30°C and 40r / min for 18h, and the spores OD of the expanded culture of the suspension 600 The value is 0.4. Wherein, the formula of enzyme-producing medium is Jerusalem artichoke powder 25g / L, (NH 4 ) 2 SO 4 5g / L,...

Embodiment 2

[0040] Ethanol was prepared by the following method:

[0041] 1. Inoculate the Saccharomyces cerevisiae strain into a 250ml Erlenmeyer flask containing 100ml seed medium according to the inoculum amount of 8%, culture it in a shaker at 30°C and 100r / min for 30 hours, and centrifuge the fermentation broth at 5000r / min for 5min. Yeast cells were collected; the seed medium was yeast extract 10 g / L, peptone 20 g / L, glucose 20 g / L, and natural pH.

[0042] 2. Make Aspergillus niger into a spore suspension with sterile water, inoculate it into a 250ml Erlenmeyer flask containing 100ml of enzyme-producing medium according to the inoculation amount of 2%, and cultivate it in a shaker at 30°C and 40r / min for 18h, and the spores OD of the expanded culture of the suspension 600 The value is 0.5. Wherein, the formula of enzyme-producing medium is Jerusalem artichoke powder 25g / L, (NH 4 ) 2 SO 4 5g / L, peptone 10g / L, KH 2 PO 4 6 g / L, NaCl 5 g / L, MgSO 4 ·7H 2 O 0.5g / L, FeSO 4 ·7H...

Embodiment 3

[0047] Study on the influence of the formation of mixed mycelium balls on the fermentation effect under different rotational speed conditions

[0048] Ethanol is prepared by fermentation according to the method of Example 1, the difference is: in step 3, when Aspergillus niger and Saccharomyces cerevisiae are mixed cultured, the shaker speed is mixed according to 80r / min, 100r / min, 180r / min, and the final obtained ethanol The performance parameters are as follows.

[0049]

[0050] Through the comparison of Example 1 and the above data, it can be seen that the rotating speed of the shaking table has a great influence on the formation of mixed mycelium balls, the yield of ethanol, and the like.

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Abstract

The invention discloses a method for preparing ethyl alcohol by adopting a carrier fermenting technique. The method comprises the following steps of: respectively culturing saccharomyces cerevisiae and aspergillus niger; inoculating the saccharomyces cerevisiae into the culture solution of the aspergillus niger and culturing to form mixed mycelium pellets; and pouring the mixed mycelium pellets into a fermentation medium and carrying out simultaneous saccharification and fermentation at the temperature of 30 DEG C and at the stirring speed of 40-60 revolutions per minute to prepare the ethyl alcohol. According to the invention, the simultaneous saccharification and fermentation process and the microorganism immobilization technique are ingeniously combined, so that the method has the advantages of reducing the viscosity of fermentation liquor, effectively preventing the bacterial contamination, reducing the COD (Chemical Oxygen Demand) value of waste grains liquid and increasing the yield of the ethyl alcohol.

Description

technical field [0001] The invention relates to a method for preparing ethanol by carrier fermentation technology, in particular to a method for fermenting ethanol by organically combining immobilized carrier as a fermentation strain with synchronous saccharification and fermentation technology. Background technique [0002] Fuel ethanol was first fermented from corn, but this process uses a large amount of corn, which poses a threat to national food security. Since 2006, my country has issued a series of policies, emphasizing that the development of fuel ethanol should adhere to the "non-grain" principle, and control the blind development of the deep processing industry of corn products. The main ingredient of Jerusalem artichoke (commonly known as Jerusalem artichoke, Jiangbula, and ghost ginger) is inulin, which is the simplest type of fructan, and its sugar content is 60% to 70% of its dry weight. The hydrolysis of Jerusalem artichoke is easier than that of starchy raw ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P7/06C12P19/14C12N11/16C12R1/865C12R1/685
CPCY02E50/17Y02E50/10
Inventor 张超陈文兵武道吉张贵芹祁锋马永山赵海涛
Owner 成都市科隆化学品有限公司
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