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Method utilizing microalgae to produce biodiesel

A technology of biodiesel and microalgae, applied in biofuel, biological raw material, petroleum industry, etc., to achieve the effect of improving transesterification efficiency, mild reaction conditions, and simple separation

Active Publication Date: 2014-03-19
BEIJING EURO & AMERICAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for preparing biodiesel with microalgae for the above-mentioned defects, so as to overcome the defects of the current traditional method for preparing biodiesel and improve the yield of biodiesel

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Microalgae culture: use Pavlova viridis as raw material to cultivate it to the logarithmic growth phase, inoculate it into fresh medium at an inoculation density of 0.3, culture at a temperature of 25°C, and culture with a light condition of 3000 lx, and collect microalgae cells , concentrated by centrifugation, freeze-dried, and then fully grind the algae powder for later use;

[0026] (2) Extraction of microalgae oil: Weigh a certain amount of algae powder, add chloroform-methanol-water mixture for extraction, the volume ratio of chloroform:methanol:water is 1:2:1.5, and the amount of algae powder added is equal to that of chloroform-methanol water The ratio is 1g:100mL, mix and stand for 4h, collect the supernatant, add chloroform water, make the ratio of chloroform:methanol:water 1:1:0.9, collect the lower chloroform layer, and distill off the chloroform at 60°C to get Microalgae oil;

[0027] (3) Enzyme-catalyzed reaction for the preparation of microalgae biod...

Embodiment 2

[0029] (1) Microalgae culture: use microalgae as raw material to cultivate it to the logarithmic growth phase, inoculate it into fresh medium at an inoculation density of 0.2, culture at a temperature of 30°C, and cultivate with a light condition of 3000 lx, and collect microalgae cells , concentrated by centrifugation, freeze-dried, and then fully grind the algae powder for later use;

[0030] (2) Extraction of microalgae oil: Weigh a certain amount of algae powder, add chloroform-methanol-water mixture for extraction, the volume ratio of chloroform:methanol:water is 1:2:0.8, and the amount of algae powder added is the same as that of chloroform-methanol water. The ratio is 1g:100mL, mix and let stand for 5h, collect the supernatant, add chloroform water, make the ratio of chloroform:methanol:water 1:1:0.9, collect the lower chloroform layer, and distill off the chloroform at 60°C to get Microalgae oil;

[0031] (3) Enzyme-catalyzed reaction for the preparation of microalgae...

Embodiment 3

[0033] (1) Microalgae culture: use Pavlova viridis as raw material to cultivate it to the logarithmic growth phase, inoculate it into fresh medium at an inoculation density of 0.4, culture at a temperature of 20°C, and cultivate with a light condition of 4000 lx, and collect microalgae cells , concentrated by centrifugation, freeze-dried, and then fully grind the algae powder for later use;

[0034] (2) Extraction of microalgae oil: Weigh a certain amount of algae powder, add chloroform-methanol-water mixture for extraction, the volume ratio of chloroform:methanol:water is 1:2:3, and the amount of algae powder added is the same as that of chloroform-methanol water. The ratio is 1g:100mL, mix and stand for 3h, collect the supernatant, add chloroform water, make the ratio of chloroform:methanol:water 1:1:0.9, collect the lower chloroform layer, and distill off the chloroform at 60°C to get Microalgae oil;

[0035] (3) Enzyme-catalyzed reaction for the preparation of microalgae ...

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PUM

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Abstract

The invention belongs to the fields of biological engineering and energy, and specifically relates to a method utilizing microalgae to produce biodiesel. The method comprises the following steps: (1) culturing microalgae; (2) extracting the microalgae fat; (3) carrying out enzyme catalytic reactions so as to prepare microalgae biodiesel. An ester exchange method is adopted during the biodiesel preparation process, lipase is taken as the catalyst, the reaction conditions are mild, free aliphatic acids can be completely and directly esterified by lipase, the alcohol using amount is little, and furthermore the method adopts composite lipase to catalyze the reactions in order to effectively overcome the shortage of substrate specificity of single lipase and improve the ester exchange efficiency of biodiesel. The biodiesels prepared by the method all meet the GB / T20828-2007 standard of biodiesel for diesel engine fuel blending.

Description

technical field [0001] The invention belongs to the field of bioengineering and energy, and in particular relates to a method for preparing biodiesel from microalgae. Background technique [0002] At present, energy and environmental issues are the focus of human attention. With the depletion of oil and coal resources and the pollution of air caused by combustion, countries around the world are actively developing and utilizing renewable new resources, such as the development and production of biodiesel . my country's biodiesel research and development started late, but the development speed is very fast. [0003] Biodiesel is a mixture of various fatty acid methyl esters. It is a methyl ester fuel made from renewable animal and vegetable oils and other alcohols through transesterification with methanol and other alcohols. It is a non-toxic renewable energy source. It is mainly composed of hydrocarbon elements, and the content of toxic gases emitted after combustion is gre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C11C3/10C10L1/02
CPCY02E50/13Y02E50/10Y02P30/20
Inventor 车春玲熊长祥
Owner BEIJING EURO & AMERICAN INST OF SCI & TECH
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