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A method for cultivating elite bulls using genome-wide selection and sex-controlled embryo technology

A whole genome and embryo technology, applied in the field of biology and new medicine, can solve the problems of poor genetic quality, mediocre good traits, and difficulty in ensuring the stability of trait selection, etc., to achieve precise breeding, improve breeding stability and accuracy Effect

Inactive Publication Date: 2016-09-28
天津市奶牛发展中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional progeny determination technology evaluates the phenotype of the trait gene. Due to the polygenic control of traits and the phenotype of the gene is easily affected by environmental factors, there are often individuals with high phenotypic values ​​that may not be stable inheritance, and individuals with low phenotypic values. It is not necessarily a problem of poor genetic quality. It is difficult to ensure the stability of trait breeding. As a result, the offspring of a world-class excellent bull breed will gradually become mediocre with the increase of generations.

Method used

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  • A method for cultivating elite bulls using genome-wide selection and sex-controlled embryo technology
  • A method for cultivating elite bulls using genome-wide selection and sex-controlled embryo technology
  • A method for cultivating elite bulls using genome-wide selection and sex-controlled embryo technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1, parental donor selection

[0024] According to certain breeding parameters, such as comprehensive breeding performance ranking in the top ten in the world, according to the male parent's CPI / GCPI breeding value, TPI / GTPI breeding value and LPI / GLPI breeding value, select the appropriate semen donor. The selection of the female parent is based on the DHI measurement and linear identification results of the cows. Select high-yield dairy cows with milk production ≥ 12 tons, milk protein rate ≥ 3.2%, milk fat ≥ 3.6%, and body size ≥ 85 in the 305 days of the first litter. According to the similarities and differences of the target focus, single trait ranking is carried out, and several top-ranked cattle are selected for genome-wide testing according to needs, and excellent individuals are selected according to genome testing and actual production performance as oocyte donors.

Embodiment 2

[0025] Example 2, making excellent sex-controlled embryos

[0026] (1) Superovulation: Donor cows undergo two PG simultaneous estrus treatments, start superovulation 9-13 days after the second simultaneous estrus, and perform artificial insemination 8-12 hours after the second estrus. The total dose of FSH super-exclusion is 8.4-8.8 mg (different doses are determined according to age, weight, etc.), intramuscular injection for 4 consecutive days, once a day in the morning and evening.

[0027] (2) Estrus identification: The estrus identification adopts the combination of external observation and rectal palpation of follicles, and the main basis is the degree of benign follicles in rectal examination. Touch the uterus and ovaries with the rectum to judge their length, thickness and texture. The sequence is: wear latex gloves on hands, apply lubricant, put five fingers together and insert into the anus, after removing feces, flatten the palms with the palms facing down, there i...

Embodiment 3

[0034] Example 3, embryo gender identification and cryopreservation

[0035] The collected excellent embryos (see attached figure 1 ), obtain single-cell specimens from embryos under a micromanipulator, put them into marked PCR tubes, add 20 microliters of protein kinase K-containing lysate for cell lysis, and the lysis program is water bath at 55 ° C for 5 min, After that, put it in a water bath at 95°C for 5 minutes. After the specimens are taken, the embryos are frozen by normal freezing procedures, sealed in thin tubes, and finally stored in liquid nitrogen for a long time.

[0036] Find a segment of gender identification fragment on the Y chromosome of cattle, use Primer5 software to design a pair of gender identification primers as follows: upstream primer: 5'-ctagctcgagatgttcagagtattgaacgacgatgt-3', downstream primer 1: 5'-gatcgcggccgcttcaatattgaaaataagcac-3'), the length of the target fragment 690bp (attached figure 2 ).

[0037]The PCR amplification conditions we...

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Abstract

The present invention provides a method for rapid and directional breeding of superior breeding bulls by using whole-genome selection and sex-controlled embryo technology, which is to select excellent cows and bulls as oocytes and semen respectively by using whole-genome selection, DHI measurement technology and descendant measurement technology Through the process of superovulation, artificial insemination and embryo collection, a large number of excellent embryos with similar genetic backgrounds are obtained, and then the sex characteristics of the embryos are obtained through in vitro embryo sex identification technology, and male embryos are selected for embryo transfer and genome breeding value determination , CPI comprehensive breeding value measurement and trait correlation analysis to realize rapid breeding and directional breeding of bulls.

Description

technical field [0001] The invention relates to a method for quickly and directionally cultivating fine bulls by using whole-genome selection and sex-controlled embryo technology, which belongs to the field of biology and new medicine, and the specific direction is modern agricultural technology. Background technique [0002] Genomic Selection (GS) technology is a resource group verified based on the conventional dairy cow genetic evaluation system. Microarray technology is used to collect the typing results of high-density genomic SNP markers in the resource group, and the basic data is constructed based on the trait breeding value to estimate each SNP. A new molecular breeding technique that marks the effect size of living haploid fragment genotypes. Compared with the traditional bull breeding technology, it has the advantages of measuring young individuals before sexual maturity, estimating the genome breeding value of young individuals, realizing early prediction and sel...

Claims

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Application Information

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IPC IPC(8): A01K67/027C12Q1/68A61D19/02A61D19/04
Inventor 马毅姚强赵庆彬
Owner 天津市奶牛发展中心
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