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Method for producing sophorolipid by double carbon source fermentation of waste molasses and illegal cooking oil

A technology of gutter oil and waste molasses, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., to achieve the effects of ensuring health, facilitating development, and high application value

Active Publication Date: 2014-02-19
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, reports on the use of waste oil as a hydrophobic carbon source focus on the detection method on the one hand, and on the esterification and saponification of biodiesel and fatty acids on the other hand. There is no report on fermentation as a substrate.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Torulopsis globosa ( Torulopsis bombicola ) ATCC 22214 was inoculated on the YPD slant medium, cultured at 30°C for 2 days, and the bacteria were eluted with 0.85% normal saline, and the cell content was about 5.3×10 7 Individual / mL bacterial suspension;

[0029] (2) Preparation of seed liquid: composition of seed medium (g / L): glucose 60, yeast powder 5, urea 0.5, pH 6.0; put 100mL of the seed medium in a 500mL shaker flask, sterilize at 121°C for 20min, cool Finally, insert the above-mentioned bacterial suspension according to the inoculation amount of 3% by volume, place the shaker flask at 25°C, and cultivate it on a shaking table with a rotation speed of 120rpm for 24h to obtain a seed solution;

[0030] (3) The above seed liquid is used to inoculate the fermentation medium of a sterile 5L fermenter with waste molasses and waste oil as substrates. Fermentation medium composition (g / L): waste oil 60, waste molasses 60, yeast powder 3, pH=6.0, tap water to a co...

Embodiment 2

[0033] (1) Torulopsis globosa ( Torulopsis bombicola ) ATCC 22214 was inoculated on YPD slant medium, cultured at 30°C for 2 days, and the bacteria were eluted with 0.85% normal saline, and the cell content was about 6.2×10 7 Individual / mL bacterial suspension;

[0034] (2) Preparation of seed liquid: composition of seed medium (g / L): glucose 80, yeast powder 6, urea 0.8, pH 6.0; put 100mL of the seed medium in a 500mL shake flask, sterilize at 121°C for 20min, cool Finally, insert the above-mentioned bacterial suspension according to the inoculation amount of 4% by volume, place the shaker flask at 28°C, and cultivate it on a shaking table with a rotation speed of 150rpm for 24h to obtain a seed solution;

[0035] (3) The above seed liquid is used to inoculate a sterile 5L fermenter with waste molasses and waste oil as substrates in the fermentation medium. Fermentation medium composition (g / L): waste oil 80, waste molasses 100, yeast powder 4, pH=6.0, tap water to a volume...

Embodiment 3

[0038] (1) Torulopsis globosa ( Torulopsis bombicola ) ATCC 22214 was inoculated on the YPD slant medium, cultured at 30°C for 2 days, and the bacteria were eluted with 0.85% normal saline, and the cell content was about 7.4×10 7 Individual / mL bacterial suspension;

[0039](2) Preparation of seed liquid: composition of seed medium (g / L): glucose 100, yeast powder 7, urea 1, pH 6.0; put 100mL of the seed medium in a 500mL shaker flask, sterilize at 121°C for 20min, cool Finally, insert the above-mentioned bacterial suspension according to the inoculation amount of 5% by volume, place the shaker flask at 30°C, and cultivate it on a shaker with a rotating speed of 180rpm for 24h to obtain a seed solution;

[0040] (3) The above seed liquid is used to inoculate a sterile 5L fermenter with waste molasses and waste oil as substrates in the fermentation medium. Fermentation medium composition (g / L): waste oil 100, waste molasses 160, yeast powder 4, pH=6.0, tap water to a volume of...

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Abstract

The invention discloses a method for producing sophorolipid by double carbon source fermentation of waste molasses and illegal cooking oil. The method is characterized by comprising the following steps: (1) inoculating torulopsis utilis to a YPD (yeast peptone dextrose) slant culture medium to prepare a bacterial suspension; (2) preparing a seed solution; (3) inoculating the seed solution to a fermentation medium taking the waste molasses and the illegal cooking oil as substrates for fermenting; (4) extracting the sophorolipid by ethyl acetate. According to the method, wastes are recycled, the production cost of the sophorolipid is greatly reduced, the problems of high emission pollution and large harm in the field of foods for the illegal cooking oil are solved, illegal cooking oil resources are reasonably utilized, complex equipment is not required, a fermentation process is simple and easy to implement, and the development, application and popularization of the sophorolipid are facilitated.

Description

technical field [0001] The invention belongs to the technical field of preparation of biosurfactants, and in particular relates to a method for fermenting and producing sophorolipids from waste molasses and waste oil as dual carbon sources. Background technique [0002] In addition to being used as detergents in daily life, surfactants can be used in almost all fine chemical fields. Sophorolipid is an environmentally friendly biosurfactant produced by microorganisms. Compared with surfactants produced by chemical synthesis or petroleum refining, it has its own advantages in addition to its common characteristics. Sophorolipids are glycolipid-based biosurfactants with low toxicity, good compatibility with the human body and the environment, and good emulsification, dispersion, and solubilization properties; they are used in food, medicine, cosmetics, metallurgy, environmental protection, waste Oil recovery and other fields have research and development value. Sophorolipids ...

Claims

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Application Information

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IPC IPC(8): C12P19/12C12R1/645
Inventor 李俊峰梁生康李红芳段效辉
Owner QINGDAO UNIV OF SCI & TECH
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