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Analysis method for five flavonoid glycosides in blood plasma and application of five flavonoid glycosides in pharmacokinetics

A technology of blood plasma and baicalin, applied in the field of modern Chinese medicine application, to achieve the effect of accurate detection results and high sensitivity

Active Publication Date: 2014-02-12
北京中一堂科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Naringin, hesperidin, neohesperidin, baicalin and wogonoside are the five active ingredients in Tangminlin, but there is no method that can simultaneously detect these five ingredients in plasma samples

Method used

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  • Analysis method for five flavonoid glycosides in blood plasma and application of five flavonoid glycosides in pharmacokinetics
  • Analysis method for five flavonoid glycosides in blood plasma and application of five flavonoid glycosides in pharmacokinetics
  • Analysis method for five flavonoid glycosides in blood plasma and application of five flavonoid glycosides in pharmacokinetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0123] LC-MS / MS conditions

[0124] Column: ZORBAX SB-C 18 (150×2.1mm, 5μm, Agilent)

[0125] Pre-column: Security Guard-C 18 (4.0mm×3.0mm i.d, 5μm, Phenomenex)

[0126] Mobile phase: 0.1% formic acid water (A)-acetonitrile (B), gradient elution program: 0~3.0min, 24%B; 3.0~3.1min, 24%B–40%B; 3.1~10.0min, 40%B B-60% B.

[0127] Column temperature: 25°C

[0128] Flow rate: 300μL·min -1

[0129] Ion source: electrospray ionization source (ESI)

[0130] Ionization mode: positive and negative ion switching mode

[0131] MS / MS: Selected Reaction Monitoring (SRM). 0~4.0min, using negative ion mode, the ions used for quantitative analysis are m / z579→271 (naringin), m / z609→301 (hesperidin and neohesperidin), m / z417→255 ( Internal standard, liquiritin); 4.1~10.0min, using positive ion mode, the ions used for quantitative analysis were m / z447→271 (baicalin), m / z461→285 (wogonin).

[0132] Mass spectrometry parameters: In negative ion mode, the parameter spray voltage is 4000V...

Embodiment 2

[0141] LC-MS / MS conditions

[0142] Column: ZORBAX SB-C 18 (150×2.1mm, 5μm, Agilent)

[0143] Pre-column: Security Guard-C 18 (4.0mm×3.0mm i.d, 5μm, Agilent)

[0144] Mobile phase: 0.01% formic acid water (A)-acetonitrile (B), gradient elution program: 0~3.0min, 30%B; 3.0~3.1min, 24%B–60%B; 3.1~10.0min, 60%B B – 80% B.

[0145] Column temperature: 20°C

[0146] Flow rate: 250μL·min -1

[0147] Ion source: electrospray ionization source (ESI)

[0148] Ionization mode: positive and negative ion switching mode

[0149] MS / MS: Selected Reaction Monitoring (SRM). 0~4.0min, using negative ion mode, the ions used for quantitative analysis are m / z579→271 (naringin), m / z609→301 (hesperidin and neohesperidin), m / z417→255 ( Internal standard, liquiritin); 4.1~10.0min, using positive ion mode, the ions used for quantitative analysis were m / z447→271 (baicalin), m / z461→285 (wogonin).

[0150] Mass spectrometry parameters: In negative ion mode, the parameter spray voltage is 4000V...

Embodiment 3

[0157] LC-MS / MS conditions

[0158] Chromatographic column: WondaSil-C18 (150×2.1mm, 5μm, Shimadzu)

[0159] Pre-column: Security Guard-C 18 (4.0mm×3.0mm i.d, 5μm, Phenomenex)

[0160] Mobile phase: 0.5% formic acid water (A)-acetonitrile (B), gradient elution program: 0~3.0min, 24%B; 3.0~3.1min, 24%B–60%B; 3.1~10.0min, 60%B b.

[0161] Column temperature: 30°C

[0162] Flow rate: 300μL·min -1

[0163] Ion source: electrospray ionization source (ESI)

[0164] Ionization mode: positive and negative ion switching mode

[0165] MS / MS: Selected Reaction Monitoring (SRM). 0~4.0min, using negative ion mode, the ions used for quantitative analysis are m / z579→271 (naringin), m / z609→301 (hesperidin and neohesperidin), m / z417→255 ( Internal standard, liquiritin); 4.1~10.0min, using positive ion mode, the ions used for quantitative analysis were m / z447→271 (baicalin), m / z461→285 (wogonin).

[0166] Mass spectrometry parameters: In negative ion mode, the parameter spray voltage ...

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Abstract

The invention provides a method for quantitatively detecting naringin, aurantiamarin, neohesperidin, baicalin and wogonoside in a blood plasma sample by adopting liquid chromatography tandem mass spectrometry, and the method comprises the following steps: (1) preparaing a sample; (2) detecting. The analysis method provided by the invention has good specificity, high precision degree, high accuracy and wide linear range and can be used for in vivo pharmacokinetic determination of Chinese medicinal compositions.

Description

technical field [0001] The invention belongs to the application field of modern traditional Chinese medicines, and in particular relates to a liquid chromatography tandem mass spectrometry detection method for five flavonoid glycosides in blood plasma and an application in pharmacokinetics. Background technique [0002] Mass spectrometry is an analysis method based on the determination of molecular weight. In recent years, its combination with separation mechanisms such as high performance liquid chromatography (HPLC) or capillary electrophoresis (CE) has not only improved its ability to resist interference from impurities and saved analysis time. time, and greatly improved the sensitivity of the assay. The development of soft ionization techniques, such as electrospray ionization (ESI), has expanded the range of molecular weight detection. In addition, electrospray ionization technology can be carried out under atmospheric pressure, which is convenient to connect with liqu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/34G01N30/72
Inventor 佟玲周水平朱永宏马晓慧靳元鹏鄂秀辉
Owner 北京中一堂科技有限公司
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