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Method of detecting human sweat secretion

A technology for detecting human body and secretions, applied in the field of biological analysis and fingerprint detection, can solve the problems of complicated operation and affect the effect of fingerprint imaging, and achieve the effect of less drug poisoning, significantly clear fingerprint image and high specificity

Active Publication Date: 2015-06-17
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Later, many scientists improved this method (for example, the Chinese patent with the publication number CN 101703404A "The method of displaying fingerprints on the surface of multiple objects and retaining DNA information"), all of which used the hydrophilicity and hydrophobicity of gold nanoparticles and fingerprints And electrostatic adsorption, but still non-specific adsorption is serious, affecting the fingerprint imaging effect, and the operation is cumbersome

Method used

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  • Method of detecting human sweat secretion
  • Method of detecting human sweat secretion
  • Method of detecting human sweat secretion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] 1. The effect of antibody solvent on fingerprint imaging

[0069] (1) Substrate treatment

[0070] The substrate is a professional fingerprint extraction tape, and its non-adhesive side is fixed on a glass slide with double-sided tape as a support.

[0071] (2) Preparation of human immunoglobulin G analog fingerprint samples:

[0072] The experimenter first washed his hands with soap, rinsed them with deionized water, and dried them with a hair dryer. They applied 25 μL of 1 mg / mL human immunoglobulin G solution on their fingers, and after drying, pressed them gently on the adhesive tape for 2 minutes to obtain a simulated protein fingerprints.

[0073] (3) Immunopolymetallic deposition imaging: draw a circle around the fingerprint with an immunohistochemical pen to form a reaction pool to avoid the diffusion of the antibody solution, and add 100 μL of 0.1 mg / mL nano-gold-labeled goat anti-human IgG to the reaction pool Antibody solution (the solvent is 0.01M PBS con...

Embodiment 2

[0116] Embodiment 2 Based on immune multi-metal deposition to visualize fingerprints and detect cotinine in fingerprints

[0117] (1) Substrate treatment

[0118] The substrate is a professional fingerprint extraction tape, and its non-adhesive side is fixed on a glass slide with double-sided tape as a support.

[0119] (2) Preparation of sweat latent fingerprint samples: Smokers wash their hands with soap, dry them with hot air, put on PE gloves to cover sweat for 5 minutes, and then press on the substrate for 1 minute to get sweat latent fingerprints.

[0120] (3) Immunopolymetallic deposition imaging: draw a circle around the fingerprint with an immunohistochemical pen to form a reaction pool to avoid the diffusion of the antibody solution, and add 100 μL of 0.1 mg / mL nano-gold-labeled cotinine antibody to the reaction pool Solution (the solvent is 0.01M PBS containing 2% BSA, 0.1% Tween-20, pH 7.4), incubate at room temperature for 15 minutes;

[0121] After incubation, ...

Embodiment 3

[0122] Embodiment 3 is based on immunological multi-metal deposition to visualize fingerprints and detect lysozyme in fingerprints

[0123] (1) Substrate treatment

[0124] The substrate is a professional fingerprint extraction tape, and its non-adhesive side is fixed on a glass slide with double-sided tape as a support. (2) Preparation of sweat latent fingerprint samples: The tester washed his hands with soap, dried them with hot air, put on PE gloves to cover his sweat for 5 minutes, and then pressed on the substrate for 1 minute to obtain sweat latent fingerprints.

[0125] (3) Immunopolymetallic deposition imaging: Draw a circle around the fingerprint with an immunohistochemical pen to form a reaction pool to avoid the diffusion of the antibody solution, and add 100 μL of 0.1 mg / mL nano-gold-labeled lysozyme antibody solution to the reaction pool (The solvent is 0.01M PBS containing 2% BSA, 0.1% Tween-20, pH 7.4), incubate at room temperature for 15 minutes;

[0126] Aft...

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Abstract

The invention discloses a method of detecting human sweat secretion. The method includes: transferring a fingerprint to a substrate to obtain a fingerprint sample; adding nano-gold labeled target secretion antibody solution to be detected to the fingerprint of the fingerprint sample, incubating, and then rinsing with rinsing solution; drying the fingerprint sample, adding slivering solution to the fingerprint, developing in the shade, rinsing and drying the fingerprint sample, and judging; if the fingerprint appears, determining the fingerprint contains the target secretion to be detected; if the fingerprint not appearing, determining the fingerprint contains no target secretion to be detected. The method of detecting the human sweat secretion is capable of detecting the target secretion simply, quickly and specifically, and also capable of identifying an object containing the target secretion.

Description

technical field [0001] The invention belongs to the technical field of biological analysis and fingerprint detection, and in particular relates to a method for detecting human sweat secretion. Background technique [0002] Fingerprints are the lines formed by the uneven skin on the pulp of the end of the human finger. They are formed and shaped during the development of the skin, and have a special physiological structure and characteristic system. In the process of human evolution, the physiological purpose of fingerprints is to increase the friction force when the hand touches objects, so that it is easier to exert force and grasp objects. Later, based on its specificity and invariance, people applied it to personal identification and forensic identification, and extended it to the fields of safety inspection and personal authentication in daily life. [0003] Fingerprints can be divided into obvious fingerprints and latent fingerprints according to whether they can be di...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61B5/117G01N33/68G01N33/532
Inventor 苏彬何亚芸
Owner ZHEJIANG UNIV
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