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Application of eIF6 in detection, prevention or treatment of exercise-induced fatigue or memory loss

A kind of usage and homology technology, applied in the direction of gene therapy, microbial measurement/testing, resistance to vector-borne diseases, etc., can solve the problem of low eIF6 protein, and achieve the effect of strong learning and spatial positioning ability

Inactive Publication Date: 2014-01-22
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

eIF6 can be detected in normal mucosal cells, but it is overexpressed in cancer cells, such as the upregulation of eIF6 in colorectal cancer[27], and it is especially obvious in lymph node metastases[28], so it is suggested that the high expression of eIF6 may be Involved in tumor proliferation and metastasis
[0019] It can be seen from the above that eIF6 plays an important role in ribosome synthesis, cytoskeleton, and tumor proliferation, differentiation and metastasis, but there are few related literatures on eIF6 protein, especially its relationship with learning and memory functions and its role in No effect on memory loss or memory impairment has been reported

Method used

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  • Application of eIF6 in detection, prevention or treatment of exercise-induced fatigue or memory loss
  • Application of eIF6 in detection, prevention or treatment of exercise-induced fatigue or memory loss
  • Application of eIF6 in detection, prevention or treatment of exercise-induced fatigue or memory loss

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Embodiment 1. Open field test detection

[0063] The open field test, also known as the open box test, is a method to evaluate the autonomous behavior, exploration behavior and tension of experimental animals in a novel environment. The frequency and duration of certain behaviors of experimental animals in novel environments are used to reflect the autonomous behavior and exploratory behavior of experimental animals in unfamiliar environments, and the tension is reflected by the frequency of urination and defecation. The experimental device consists of two parts: an open field reaction box and an automatic data acquisition and processing system. In a quiet experimental environment, 12-13 8-10-week-old male eIF6 knockout mice (eIF6 single gene knockout mice imported from Italy, bred in SPF grade animal room; eIF6 knockout mice homozygous Lethal, heterozygous mRNA and protein expression are reduced by half) and wild-type mice with the same genetic background are lightly ...

Embodiment 2

[0064] Embodiment 2. Rotating Rod Type Fatigue Tester

[0065] The mouse rotary rod fatigue tester is designed according to the physiological characteristics of mice that are good at climbing but not good at running. The instrument can be used in the research of sports medicine, anti-fatigue drugs, muscle relaxant drugs, nervous system drugs, research on the changes of physiological indexes of animals under different exercise intensities, and research on the influence of drug toxicity on the exercise performance of experimental animals , 6 mice can be tested at the same time, the experimental conditions are consistent, and the experimental indicators are accurate. Put 12-13 8-10 week-old male eIF6 knockout mice and wild-type mice with the same genetic background on the rotarod, and insert the rotarod baffle. The mice were acclimatized for 20 minutes at a speed of 20 rpm / min, and the time the mice stayed on the rotarod at 30 rpm / min was recorded.

Embodiment 3

[0066] Example 3. Water maze test

[0067] The experiment lasted 4 days. Half an hour before the start of the experiment, the mice were placed in the room where the water maze was located to adapt to the environment. Each mouse was trained 4 times a day, and each time the water entry point was selected from four quadrants in a semi-random manner. The experimenter lifted the mouse with his hand to face the pool wall, and gently put it into the water. The mouse can find the platform within 90s, then record the time it needs to search and board the platform, ie the latency (latency). If the mouse failed to find the platform within 90s, the experimenter guided it to the platform by hand, and the latency was recorded as 90s. After the mouse boarded the platform, let it stay on the platform for 15 seconds, so that it can perform spatial learning and memory according to the reference objects of the four quadrants, and reduce the tension of the mouse. After each training session, d...

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Abstract

The invention relates to an application of beta4 integrin binding protein (eIF6), or a protein an eIF6 protein derivative having an amino acid sequence of an eIF6 protein and ligand binding site and / or the functional site of the eIF6 protein, a nucleic acid molecule containing an eIF6 gene nucleotide sequence, or a nucleic acid molecule for effectively suppressing eIF6 gene expression and a DNA (deoxyribonucleic acid) expression vector containing the nucleic acid molecule in the preparation of medicines for detecting, preventing or treating exercise-induced fatigue, memory loss, or related diseases and detection kits of related diseases.

Description

technical field [0001] The present invention relates to β4 integrin binding protein (Integrin beta 4 binding protein, eIF6), or a protein or eIF6 protein derivative having the amino acid sequence of the binding site of eIF6 protein and ligand and / or the functional site of eIF6, comprising eIF6 gene nucleic acid The nucleic acid molecule of sequence or the nucleic acid molecule effectively inhibiting the expression of eIF6 gene, as well as the DNA expression vector comprising the nucleic acid molecule, are used for detecting, preventing or treating exercise-induced fatigue, memory loss or memory impairment. Background technique [0002] 1. Exercise fatigue [0003] Exercise-induced fatigue mainly refers to the phenomenon of decreased physical ability caused by exercise. In 1982, the Fifth International Sports Biochemical Conference unified the definition of fatigue, and exercise-induced fatigue is "the body's physiological process cannot continue its function at a certain le...

Claims

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Application Information

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IPC IPC(8): A61K38/17A61K48/00C12Q1/68A61P1/16A61P3/10A61P5/14A61P9/10A61P11/02A61P25/08A61P25/14A61P25/16A61P25/18A61P25/20A61P25/24A61P25/28A61P35/00A61P39/02A61P39/06
CPCY02A50/30
Inventor 崔艳艳吴军罗高兴桂莉谭江琳贺伟峰
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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