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Extraction method and application of resveratrol

A technology of resveratrol and extraction method, applied in chemical instruments and methods, preparation of organic compounds, bone diseases, etc., can solve problems affecting clinical application, high extraction cost, and many impurities

Inactive Publication Date: 2014-01-01
NANJING ZHENGLIANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Resveratrol is in great demand in the market due to its low content in plants and high cost of extraction
[0003] In the prior art, there is no report on the combined application of resveratrol using supercritical and microwave technology in the extraction and preparation. However, the current extraction method has rough and backward technology and many impurities, which leads to excessive dosage for patients and is inconvenient to take. Seriously affected the clinical application of this product

Method used

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  • Extraction method and application of resveratrol
  • Extraction method and application of resveratrol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Take Polygonum cuspidatum 1000g, add to CO 2 In a supercritical extractor, ethanol is used as an entrainer, and the entrainer accounts for 4% by volume of the total extraction solvent. The extraction pressure is 15MPa, the temperature is 30°C, and the CO 2 The flow rate is 1m1 / g crude drug·min, the extraction time is 150min, and the supercritical extract is obtained, then 70% ethanol is added, and put into the microwave extraction device for microwave extraction, the extraction power is 400W, and the extraction is performed twice, each time for 4 minutes, and each time the added The amount of 70% ethanol is 2000g, the extract is obtained, concentrated, added to a D101 macroporous adsorption resin column, eluted with 50% ethanol, collected 5 times the column volume eluate, recovered ethanol under reduced pressure, concentrated and dried to obtain white Veratrol extract, the content of resveratrol determined by high performance liquid chromatography is 97.3%.

Embodiment 2

[0020] Take Polygonum cuspidatum 1000g, add to CO 2 In a supercritical extractor, ethanol is used as an entrainer, and the volume percentage of the entrainer in the total extraction solvent is 6%, the extraction pressure is 30MPa, the temperature is 50°C, and the CO 2 The flow rate is 3m1 / g crude drug·min, the extraction time is 180min, and the supercritical extract is obtained, then 70% ethanol is added, and put into a microwave extraction device for microwave extraction, the extraction power is 600W, and the extraction is performed twice, each time for 8 minutes, and each time the added The amount of 70% ethanol is 2000g, the extract is obtained, concentrated, added to a D101 macroporous adsorption resin column, eluted with 50% ethanol, collected 5 times the column volume eluate, recovered ethanol under reduced pressure, concentrated and dried to obtain white Veratrol extract, the content of resveratrol determined by high performance liquid chromatography is 97.5%.

Embodiment 3

[0022] Take Polygonum cuspidatum 1000g, add to CO 2 In the supercritical extractor, ethanol is used as an entrainer, and the entrainer accounts for 5% by volume of the total extraction solvent, the extraction pressure is 20MPa, the temperature is 40°C, and the CO 2 The flow rate is 2m1 / g crude drug·min, the extraction time is 160min, and the supercritical extract is obtained, then 70% ethanol is added, and put into the microwave extraction device for microwave extraction, the extraction power is 500W, and the extraction is performed twice, each time for 6 minutes, and each time the added The amount of 70% ethanol is 2000g, the extract is obtained, concentrated, added to a D101 macroporous adsorption resin column, eluted with 50% ethanol, collected 5 times the column volume eluate, recovered ethanol under reduced pressure, concentrated and dried to obtain white Resveratrol extract, the content of resveratrol determined by high performance liquid chromatography is 98.1%.

[002...

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Abstract

The invention provides an extraction method of resveratrol, which is obtained by supercritical extraction and microwave extraction. Thus, the content is greatly improved. The invention also provides application of resveratrol in preparation of drugs inhibiting mouse mastocytoma P815 cell proliferation and in preparation of drugs increasing bone mineral density.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine extraction, in particular to an extraction method and application of resveratrol. Background technique [0002] Resveratrol is a polyphenolic compound with the chemical name (E)-3,5,4-trihydroxystilbene. It is a non-flavonoid polyphenolic compound with the molecular formula C 14 h 12 o 3 , with a relative molecular mass of 228.25, is a white needle-like crystal, easily soluble in ether, chloroform, methanol, ethanol, acetone, acetic acid, ethyl ester and other organic solvents, mainly derived from grapes (red wine), knotweed, peanuts, mulberries and other plants . Resveratrol is a kind of natural polyphenols with strong biological properties, also known as stilbene triphenols. chemopreventive agents. The research results of the US Department of Agriculture show that the red coat and kernel of peanuts also contain quite a lot of resveratrol. Experimental studies of resve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C39/21C07C37/70A61K31/05A61P35/00A61P19/10
CPCA61K31/05C07C37/685C07C37/70C07C37/72C07C39/21Y02P20/54
Inventor 不公告发明人
Owner NANJING ZHENGLIANG MEDICAL TECH
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