Kit and method for detecting polymorphism of CYP3A4 gene

Abstract

The invention relates to a kit and a method for detecting the polymorphism of a CYP3A4 gene and belongs to the technical field of gene sequencing. The kit comprises primers for detection, wherein the primers for the detection comprise at least one pair in long-fragment amplification specific forward and reverse primers for a first exon to a third exon of the CYP3A4 gene, long-fragment amplification specific forward and reverse primers for a fourth exon to a seventh exon of the CYP3A4 gene, long-fragment amplification specific forward and reverse primers for an eighth exon to an eleventh exon of the CYP3A4 gene and long-fragment amplification specific forward and reverse primers for a twelfth exon and a thirteenth exon of the CYP3A4 gene and at least one pair in forward and reverse sequencing primers for each exon of the CYP3A4 gene corresponding to long-fragment deoxyribose nucleic acid (DNA) of the first exon to the third exon, long-fragment DNA of the fourth exon to the seventh exon, long-fragment DNA of the eighth exon to the eleventh exon and long-fragment DNA of the twelfth exon and the thirteenth exon. According to the kit and the method, the detection time is short and the workload is low.

Description

technical field [0001] The invention relates to the technical field of gene sequencing, in particular to a kit and method for detecting CYP3A4 gene polymorphism. Background technique [0002] Cytochrome P450 (CYP), also known as mixed function oxidase, monooxygenase or drug metabolizing enzyme, is widely distributed in animals, plants and microorganisms. More than 20 isoenzymes have been found in mammalian tissues, which are divided into 4 families of CYP1-4. CYP3A-type enzymes account for 70% of the total CYP450 in the body, 30% of the CYP450 in the liver, and participate in the metabolic transformation of more than 60% of commonly used drugs in the body. At present, four CYP3A~ genes have been found in the human body, namely CYP3A4, CYF3A5, CYP3A7 and CYP3A43, and CYP3A4 is one of the most clinically important P450 enzymes, involved in the oxidative metabolism of more than 150 drugs in 38 categories, Accounting for about 50% of all drugs, it is the root cause of adverse ...

AI Technical Summary

Problems solved by technology

[0005] In view of the problems in the prior art that the detection of CYP3A4 gene polymorphism takes a long time, the steps are cumbersome, and multiple PCRs are required, the purpose of the present invention is to provide a kit for detecting CYP3A4 gene polymorphism

[0007] In order to solve the problems of the prior art detection of CYP3A4 gene polymorphism, which takes a long time, the steps are cumbersome, and multiple PCRs are required, the present invention adopts the following technical solutions:

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