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Porcine circovirus type II strain

A porcine circovirus and strain technology, applied in the direction of virus/bacteriophage, microorganism, biochemical equipment and methods, etc., can solve the problem of variable reaction results of animal models of virulent infection, low PCV2 virus proliferation titer, and weak virus proliferation ability And other issues

Inactive Publication Date: 2013-12-11
HENAN AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

In the development of vaccines, the biggest difficulty encountered is the low titer of PCV2 virus proliferation
The ability of viruses to grow in vitro is weak, and it is difficult to meet the requirements of vaccine production
In addition, there is no fixed vaccine immune effect evaluation standard, and the response results of virulent infection animal models are inconsistent

Method used

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  • Porcine circovirus type II strain
  • Porcine circovirus type II strain
  • Porcine circovirus type II strain

Examples

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Embodiment Construction

[0016] A porcine circovirus type Ⅱ strain, porcine circovirus type Ⅱ (Circoviridae), CCTCC NO: V201312.

[0017] Isolation, identification and whole genome sequence analysis of porcine circovirus type Ⅱ PCV2 / HZ09 strain

[0018] 1 Materials and methods

[0019] 1.1 Test material

[0020] In August 2009, tissues such as lymph nodes, lungs, and spleen were collected from pigs suspected of having post-weaning multisystem failure syndrome in a pig farm in Zhengzhou. aq DNA polymerase, DL2000 DNA Marker, pMD18-T vector, X-gal (5-bromo-4-chloro-3-indole-β-D-galactoside) and IPTG (isopropyl-β-D-thio Galactose Substitute) etc. were purchased from Biotech (Dalian) Co., Ltd.; Glue Recovery Kit was purchased from BioTeke Company; RPMI Medium 1640 and newborn bovine serum were purchased from Suleibao Company; rabbit anti-pig IgG-FITC and D-Glucosamine were purchased from From Sigma Company; specific anti-PCV2 antiserum was purchased from VMRD Company.

[0021] 1.2 Primer design

[00...

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Abstract

The present invention discloses a porcine circovirus type II strain, wherein the latin name is Circoviridae, and the preservation number is CCTCC NO:V201312. According to the invention, in vitro PCV2 culture proliferation conditions are optimized, and a PK15 cell clone strain with high PCV2 sensitivity and named L8 is screened so as to establish a foundation for further PCV2 researches.

Description

technical field [0001] The invention relates to a type Ⅱ strain of porcine circovirus. Background technique [0002] In 1974, a German researcher discovered a new, non-pathogenic microribonucleotide virus-like pollutant from the pig kidney cell line PK15 (ATCC-CCL33). It was subsequently reported that the pollutant was an icosahedral virus with no envelope and a single-stranded circular DNA genome, which was the smallest known virus capable of self-replication in mammalian cells, and was subsequently named pig round Cyclovirus (PCV). It mainly infects monocyte / macrophage lineage cells, among which porcine alveolar macrophages are its main target cells. Subsequent serological surveys of PCV antibodies in pigs in Germany, Canada, Great Britain and Northern Ireland showed that PCV is ubiquitous in pig herds. Experimental infection of 9-month-old pigs with PCV resulted in no clinical disease and the virus was therefore considered non-pathogenic. [0003] In the late 1990s, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12R1/93
Inventor 崔保安陈红英卢权威郭官鹏郭敬李新生魏战勇
Owner HENAN AGRICULTURAL UNIVERSITY
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