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Morphine/heroin vaccine formed by covalently binding hapten and carrier, and application thereof

A heroin and antigen technology, applied in the field of vaccines, can solve problems such as research lag, and achieve high specificity, affinity, and long-lasting effects

Active Publication Date: 2013-11-27
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current vaccine research against opioid addiction is relatively lagging behind, and an optimized morphine / heroin vaccine with higher antibody concentration and longer duration is urgently needed

Method used

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  • Morphine/heroin vaccine formed by covalently binding hapten and carrier, and application thereof
  • Morphine/heroin vaccine formed by covalently binding hapten and carrier, and application thereof
  • Morphine/heroin vaccine formed by covalently binding hapten and carrier, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1, the preparation of morphine-TFCS-KLH antigen

[0052] 1, the preparation of morphine-6-glutaric acid monoester (preparation process such as figure 1 shown)

[0053] See the following patents: the application number is 201010512377.7, the title of the invention is an antigen and its preparation method and application, and the authorized announcement number is CN102002049B. details as follows:

[0054] (1) Dissolve 1.3g of morphine hydrochloride in 30mL of water, add ammonia water dropwise to pH 8.5, react at a temperature of 37°C with stirring for 10min, filter with suction to obtain an off-white solid, and dry it in vacuum for 24h to obtain 1g of free morphine alkali.

[0055] (2) Mix 1 g of morphine free base and 1.2 g of glutaric anhydride obtained in step (1) with a catalytic amount of N-dimethylaminopyridine, add 30 mL of benzene, heat and reflux at 95°C for 4 hours, and distill off under reduced pressure Solvent benzene, collect the residue; the ...

Embodiment 2

[0083] Embodiment 2, the application of the morphine-TFCS-KLH antigen prepared by embodiment 1

[0084] 1. Actively immunize rats

[0085] Male SD rats were randomly divided into two groups (9-10 weeks old, 220-240g), with 6 rats in each group, and were treated as follows:

[0086] A. Control group: subcutaneous injection of adjuvant + KLH;

[0087] B. Experimental group: Subcutaneous injection of adjuvant + morphine-TFCS-KLH antigen.

[0088] The dose of KLH or morphine-TFCS-KLH antigen was 100 μg / rat, the injection volume was 0.5 ml / rat, and the drug was divided into four points on the back and subcutaneously injected.

[0089] Thereafter, the vaccine was injected every 14 days, for a total of 4 injections, and 10 days after each injection, blood was collected from the sublingual vein, centrifuged, and serum was obtained, which was stored at -80°C for antibody titer determination. Freund's complete adjuvant was used for the first injection (KLH or morphine-TFCS-KLH antige...

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Abstract

The invention discloses an antigen and the application thereof. The antigen provided by the invention is shown as the formula I. The antigen takes morphine-6-glutaric acid monoester as hapten, the hapten and carrier protein are connected through a 6-carbon-atomic-link-shaped connecting arm of N-(Epsilon-trifluoro acetyl hexanoyl oxygen) succinimide ester (TFCS) derivant, and the distance between a morphine skeletal structure and the carrier protein is increased. The antigen can irritate a body to produce high-concentration morphine antibody, the duration of the antibody is long, and the antibody can be specifically bound with morphine and heroin and can prevent recrudescence of a heroin seeking behavior induced by ignition of heroin for a long time.

Description

technical field [0001] The invention belongs to the field of vaccines and relates to an antigen and its application, in particular to a morphine / heroin vaccine in which a hapten is covalently combined with a carrier and its application. Background technique [0002] Drug addiction is a major public health and social problem that plagues global human health and social development. It seriously endangers human health, affects family stability and social development, and causes huge economic losses to society. According to the United Nations World Drug Report in 2011, the number of drug abusers in the world reached 363 million; the "China Anti-Drug Report" shows that as of 2011, the number of registered drug users in my country reached 1.794 million, and the actual number is multiples of this. At the same time, drug abusers are high-risk groups carrying HIV, hepatitis B virus (HBV), hepatitis C virus (HCV) and other viruses. The 2011 "China AIDS Epidemic Report" shows that the...

Claims

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Application Information

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IPC IPC(8): C07K14/795C07K14/77C07K14/765C07K14/75C07K14/47C07K14/33C07K14/195C07K16/00C07K1/107A61K39/385A61P25/30C07K16/44
Inventor 陆林孙成玉李芊芊罗宜孝徐凌志
Owner PEKING UNIV
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