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Thermo-sensitive type L-carnitine molecularly-imprinted polymer and preparation method thereof

A technology of molecular imprinting and molecular imprinting microspheres, which is applied in the preparation of organic compounds, cyanide reaction preparation, chemical instruments and methods, etc., can solve problems such as muscle soreness and muscle atrophy, and achieve low production cost and simple preparation process Effect

Inactive Publication Date: 2013-09-25
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies have shown that only L-carnitine has physiological activity, while D-carnitine has a competitive inhibitory effect on carnitine acetyltransferase (CAT) and carnitine fatty acyltransferase (PTC), inhibiting mitochondrial fatty acid oxidation and energy production. Formation, overdose may also cause muscle soreness, which may seriously cause muscle atrophy. Therefore, with the increase of consumers' safety awareness, DL-carnitine has been eliminated, and the production of optically pure L-carnitine has been paid more and more attention.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Preparation of temperature-sensitive L-carnitine molecularly imprinted polymer: put 1 mmol (0.1612 g) of template molecule L-carnitine into a 50 mL ground-mouth conical flask, add 5 mL of methanol, and ultrasonicate for 3 minutes to fully dissolve it. Then add 15ml of chloroform and 4mmol (0.453g) of N-isopropylacrylamide (NIPAM), shake at a constant temperature in an air bath at 40°C for 2 hours to fully combine the template molecules with the functional monomer, take out the Erlenmeyer flask and add 5mmol (0.95mL) Cross-linking agent ethylene glycol dimethacrylate (EGDMA) and 20 mg initiator azobisisobutyronitrile (AIBN), blow nitrogen for 5 minutes to remove oxygen molecules, cover the Erlenmeyer flask with a stopper, and put it in an air bath for constant temperature In the shaker, the temperature was raised to 50°C, and the polymerization was heated for 48 hours. After the polymerization is completed, a white disc-shaped bulk polymer is obtained, which is taken ...

Embodiment 2

[0027] 1. Same as embodiment 1.

[0028] 2. Elution of imprinted template molecules: Ultrasonic extraction is used, the extraction solution is methanol solution, and the methanol solution is changed every 30 minutes. The amount of methanol used each time is 100mL, and the total elution time is 300 minutes. After the extraction, rinse with acetone for 1 Once, remove a small amount of fine polymer fragments. Vacuum dry at 40°C for 24h.

Embodiment 3

[0030]1. Preparation of temperature-sensitive L-carnitine molecularly imprinted polymer: put 1 mmol (0.1612 g) of template molecule L-carnitine into a 50 mL ground-mouth conical flask, add 5 mL of methanol, and ultrasonicate for 3 minutes to fully dissolve it. Then add 15ml of chloroform and 4mmol (0.453g) of N-isopropylacrylamide (NIPAM), shake at a constant temperature in an air bath at 40°C for 2 hours to fully combine the template molecules with the functional monomer, take out the Erlenmeyer flask and add 10mmol (1.93mL) Cross-linking agent ethylene glycol dimethacrylate (EGDMA) and 20 mg initiator azobisisobutyronitrile (AIBN), blow nitrogen for 5 minutes to remove oxygen molecules, cover the Erlenmeyer flask with a stopper, and put it in an air bath for constant temperature In the shaker, the temperature was raised to 50°C, and the polymerization was heated for 48h. After the polymerization is completed, a white disc-shaped bulk polymer is obtained, which is taken out f...

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PUM

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Abstract

The invention relates to the field of intelligent high polymer materials and discloses a thermo-sensitive type L-carnitine molecularly-imprinted polymer and a preparation method thereof. According to the invention, L-carnitine is used as a template molecule, N-isopropylacrylamide is used as a functional monomer, and the thermo-sensitive type L-carnitine molecularly-imprinted polymer and a thermo-sensitive type L-carnitine molecularly-imprinted microsphere are prepared by respectively using traditional and surface molecular imprinting technology. The preparation method comprises the following steps: 1, preparation of the thermo-sensitive type L-carnitine molecularly-imprinted polymer and 2, preparation of the thermo-sensitive type L-carnitine molecularly-imprinted microsphere, wherein the step 1 comprises a substep of preparation of the thermo-sensitive type L-carnitine molecularly-imprinted polymer and elution of the imprinting template molecule, and the step 2 comprises a first substep of preparation of a SiO2 nanometer microsphere by using a Stober method, a second substep of surface activation of the SiO2 microsphere, a third substep of surface modification of the SiO2 microsphere, a fourth substep of preparation of the thermo-sensitive type L-carnitine molecularly-imprinted microsphere and a fifth substep of elution of the imprinting template molecule. The preparation method provided by the invention has the advantages of easiness, low cost, convenience in industrial popularization and application, etc. The molecularly-imprinted polymer is applicable to separation of enantiomers of DL-carnitine.

Description

technical field [0001] The invention relates to the field of intelligent polymer materials, in particular to a class of temperature-sensitive molecularly imprinted polymers with good adsorption and separation functions for L-carnitine molecules and a preparation method thereof. The molecularly imprinted polymer can be applied in fields such as the separation of carnitine chiral drugs. Background technique [0002] L-carnitine (L-carnitine), also known as L-carnitine or carnitine, is a kind of amino acid that promotes the conversion of fat into energy. It has the effect of reducing body fat and weight without reducing water and muscle. It is safe Non-toxic, so in 2003 by the International Obesity Health Organization identified as the most safe weight loss nutritional supplements without side effects. However, studies have shown that only L-carnitine has physiological activity, while D-carnitine has a competitive inhibitory effect on carnitine acetyltransferase (CAT) and carn...

Claims

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Application Information

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IPC IPC(8): C08F222/14C08F220/54C08J9/26C08F292/00B01J20/26B01J20/30C07C229/22C07C227/34
Inventor 陈娇娇侯蔺桐薛静程立伟傅宏俊
Owner TIANJIN POLYTECHNIC UNIV
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